1887

Abstract

The taxonomic positions of species with validly published names and a novel strain Ko07, which was newly isolated from an upflow anaerobic sludge blanket reactor treating wastewater from a brewery, were (re)estimated on the basis of results obtained by using a polyphasic taxonomy approach. Phylogenetic inference based on 16S rRNA gene sequences showed that strain Ko07 and all species with validly published names clustered together in a phylogenetic branch within the class ‘’. The sequence similarity of strain Ko07 to the type strains of established species was in the range 94·9–96·7 %. Ubiquinone Q-8 and branched fatty acids, C iso, C iso, C iso, iso C 9 and C iso 3OH, predominantly appeared in strain Ko07 as well as in all type strains of the recognized species. The DNA–DNA hybridization values of strain Ko07 with those of recognized species were estimated to be 2–20 %. Despite sharing common taxonomic features in important phenotypic characteristics, such as gliding movement, long-rod shape and proteolytic activity, strain Ko07 could be distinguished from the species with validly published names by its low DNA–DNA hybridization value, a comparatively low DNA G+C content (63·8 mol%), substrate utilization and some physiochemical characteristics. On the basis of the results obtained in this study, it is proposed that strain Ko07 should be classified as representing a novel member of the genus , for which the name sp. nov. is proposed. The type strain is Ko07 (=KCTC 12205=DSM 16239).

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2005-05-01
2019-10-21
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vol. , part 3, pp. 1155 - 1161

16S rRNA gene sequence identity data for strains included in this study. [PDF](15 KB)



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