1887

Abstract

A novel, thermophilic, obligately chemolithoautotrophic, sulfur/thiosulfate-oxidizing bacterium was isolated from subsurface geothermal aquifer water (temperature approximately 70 °C) in the Hishikari gold mine, Japan. Cells of the isolate, designated strain C55, were motile, straight rods with a single polar flagellum. Growth was observed at temperatures between 35 and 62 °C (optimum 50–55 °C; 60 min doubling time) and pH between 5·2 and 7·7 (optimum pH 6·5–7·0). High growth rate of strain C55 was observed on either thiosulfate or elemental sulfur as a sole energy source, with molecular oxygen as the only electron acceptor. None of the organic compounds tested supported or stimulated growth of strain C55. The G+C content of the genomic DNA was 66·9 mol%. Phylogenetic analysis based on 16S rRNA gene sequences indicated that strain C55 was affiliated to the -, but was distantly related to recognized genera. On the basis of its physiological and molecular properties, strain C55 (=JCM12421=DSM 16629=ATCC BAA-941) is proposed as the type strain of gen. nov., sp. nov.

Loading

Article metrics loading...

/content/journal/ijsem/10.1099/ijs.0.63389-0
2005-01-01
2020-01-22
Loading full text...

Full text loading...

/deliver/fulltext/ijsem/55/1/ijs550467.html?itemId=/content/journal/ijsem/10.1099/ijs.0.63389-0&mimeType=html&fmt=ahah

References

  1. Balch, W. E., Fox, G. E., Magrum, L. J., Woese, C. R. & Wolfe, R. S. ( 1979; ). Methanogens: reevaluation of a unique biological group. Microbiol Rev 43, 260–296.
    [Google Scholar]
  2. Baross, J. A. ( 1995; ). Isolation, growth and maintenance of hyperthermophiles. In Archaea: a Laboratory Manual, Thermophiles, pp. 15–23. Edited by F. T. Robb & A. R. Place. Cold Spring Harbor, NY: Cold Spring Harbor Laboratory.
  3. Caldwell, D. E., Caldwell, S. J. & Laycock, J. P. ( 1976; ). Thermothrix thioparus gen. et sp. nov., a facultatively anaerobic facultative chemolithotroph living at neutral pH and high temperature. Can J Microbiol 22, 1509–1517.[CrossRef]
    [Google Scholar]
  4. Hayashi, N. R., Ishida, T., Yokota, A., Kodama, T. & Igarashi, Y. ( 1999; ). Hydrogenophilus thermoluteolus gen. nov., sp. nov., a thermophilic, facultatively chemolithoautotrophic, hydrogen-oxidizing bacterium. Int J Syst Bacteriol 49, 783–786.[CrossRef]
    [Google Scholar]
  5. Huber, R., Eder, W., Heldwein, S., Wanner, G., Huber, H., Rachel, R. & Stetter, K. O. ( 1998; ). Thermocrinis ruber gen. nov., sp. nov., a pink-filament-forming hyperthermophilic bacterium isolated from Yellowstone National Park. Appl Environ Microbiol 64, 3576–3583.
    [Google Scholar]
  6. Hugenholtz, P., Pitulle, C., Hershberger, K. L. & Pace, N. R. ( 1998; ). Novel division level bacterial diversity in a Yellowstone hot spring. J Bacteriol 180, 366–376.
    [Google Scholar]
  7. Inagaki, F., Takai, K., Hirayama, H., Yamato, Y., Nealson, K. H. & Horikoshi, K. ( 2003; ). Distribution and phylogenetic diversity of the subsurface microbial community in a Japanese epithermal gold mine. Extremophiles 7, 307–317.[CrossRef]
    [Google Scholar]
  8. Izawa, E., Urashima, Y., Ibaraki, K., Suzuki, R., Yokoyama, T., Kawasaki, K., Koga, A. & Taguchi, S. ( 1990; ). The Hishikari gold deposit: high-grade epithermal veins in Quaternary volcanics of southern Kyushu, Japan. J Geochem Explor 36, 1–56.[CrossRef]
    [Google Scholar]
  9. LaPara, T. M., Nakatsu, C. H., Pantea, L. & Alleman, J. E. ( 2000; ). Phylogenetic analysis of bacterial communities in mesophilic and thermophilic bioreactors treating pharmaceutical wastewater. Appl Environ Microbiol 66, 3951–3959.[CrossRef]
    [Google Scholar]
  10. Manaia, C. M., Nogales, B. & Nunes, O. C. ( 2003; ). Tepidiphilus margaritifer gen. nov., sp. nov., isolated from a thermophilic aerobic digester. Int J Syst Evol Microbiol 53, 1405–1410.[CrossRef]
    [Google Scholar]
  11. Marmur, J. & Doty, P. ( 1962; ). Determination of the base composition of deoxyribonucleic acid from its thermal denaturation temperature. J Mol Biol 5, 109–118.[CrossRef]
    [Google Scholar]
  12. Mechichi, T., Stackebrandt, E., Gad'on, N. & Fuchs, G. ( 2002; ). Phylogenetic and metabolic diversity of bacteria degrading aromatic compounds under denitrifying conditions, and description of Thauera phenylacetica sp. nov., Thauera aminoaromatica sp. nov., and Azoarcus buckelii sp. nov. Arch Microbiol 178, 26–35.[CrossRef]
    [Google Scholar]
  13. Moreira, C., Rainey, F. A., Nobre, M. F., da Silva, M. T. & da Costa, M. S. ( 2000; ). Tepidimonas ignava gen. nov., sp. nov., a new chemolithoheterotrophic and slightly thermophilic member of the β-Proteobacteria. Int J Syst Evol Microbiol 50, 735–742.[CrossRef]
    [Google Scholar]
  14. Odintsova, E. V., Jannasch, H. W., Mamone, J. A. & Langworthy, T. A. ( 1996; ). Thermothrix azorensis sp. nov., an obligately chemolithoautotrophic, sulfur-oxidizing, thermophilic bacterium. Int J Syst Bacteriol 46, 422–428.[CrossRef]
    [Google Scholar]
  15. Reysenbach, A.-L., Wickham, G. S. & Pace, N. R. ( 1994; ). Phylogenetic analysis of the hyperthermophilic pink filament community in Octopus Spring, Yellowstone National Park. Appl Environ Microbiol 60, 2113–2119.
    [Google Scholar]
  16. Shooner, F., Bousquet, J. & Tyagi, R. D. ( 1996; ). Isolation, phenotypic characterization, and phylogenetic position of a novel, facultatively autotrophic, moderately thermophilic bacterium, Thiobacillus thermosulfatus sp. nov. Int J Syst Bacteriol 46, 409–415.[CrossRef]
    [Google Scholar]
  17. Stöhr, R., Waberski, A., Liesack, W., Völker, H., Wehmeyer, U. & Thomm, M. ( 2001; ). Hydrogenophilus hirschii sp. nov., a novel thermophilic hydrogen-oxidizing β-proteobacterium isolated from Yellowstone National Park. Int J Syst Evol Microbiol 51, 481–488.
    [Google Scholar]
  18. Takai, K., Komatsu, T. & Horikoshi, K. ( 2001; ). Hydrogenobacter subterraneus sp. nov., an extremely thermophilic, heterotrophic bacterium unable to grow on hydrogen gas, from deep subsurface geothermal water. Int J Syst Evol Microbiol 51, 1425–1435.
    [Google Scholar]
  19. Takai, K., Hirayama, H., Sakihama, Y., Inagaki, F., Yamato, Y. & Horikoshi, K. ( 2002; ). Isolation and metabolic characteristics of previously uncultured members of the order Aquificales in a subsurface gold mine. Appl Environ Microbiol 68, 3046–3054.[CrossRef]
    [Google Scholar]
  20. Takai, K., Kobayashi, H., Nealson, K. H. & Horikoshi, K. ( 2003; ). Sulfurihydrogenibium subterraneum gen. nov., sp. nov., from a subsurface hot aquifer. Int J Syst Evol Microbiol 53, 823–827.[CrossRef]
    [Google Scholar]
  21. Tamaoka, J. & Komagata, K. ( 1984; ). Determination of DNA base composition by reversed-phase high-performance liquid chromatography. FEMS Microbiol Lett 25, 125–128.[CrossRef]
    [Google Scholar]
  22. Tarlera, S. & Denner, E. B. M. ( 2003; ). Sterolibacterium denitrificans gen. nov., sp. nov., a novel cholesterol-oxidizing, denitrifying member of the β-Proteobacteria. Int J Syst Evol Microbiol 53, 1085–1091.[CrossRef]
    [Google Scholar]
  23. Wood, A. P. & Kelly, D. P. ( 1988; ). Isolation and physiological characterisation of Thiobacillus aquaesulis sp. nov., a novel facultatively autotrophic moderate thermophile. Arch Microbiol 149, 339–343.[CrossRef]
    [Google Scholar]
  24. Yamamoto, H., Hiraishi, A., Kato, K., Chiura, H. X., Maki, Y. & Shimizu, A. ( 1998; ). Phylogenetic evidence for the existence of novel thermophilic bacteria in hot spring sulfur-turf microbial mats in Japan. Appl Environ Microbiol 64, 1680–1687.
    [Google Scholar]
  25. Zillig, W., Holz, I., Janekovic, D. & 7 other authors ( 1990; ). Hyperthermus butylicus, a hyperthermophilic sulfur-reducing archaebacterium that ferments peptides. J Bacteriol 172, 3959–3965.
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/ijsem/10.1099/ijs.0.63389-0
Loading
/content/journal/ijsem/10.1099/ijs.0.63389-0
Loading

Data & Media loading...

Supplements

Electron micrographs of negatively stained cells (A) and thin-section (B) of strain C55 . Bars, 0.5 µm (A), 0.2 µm (B).

IMAGE

Effects of temperature (C), pH (D) and Na concentration (E) in TSmj medium on the growth of strain C55 . Growth curves at different temperatures were determined in TSmj medium at pH 6.5. The effect of pH on growth was determined at 55 °C over a range of pH values. The pH was adjusted to various levels at room temperature. The effect of Na concentration on growth was determined in variously diluted or concentrated TSmj media. [PDF](60 KB)

PDF

Most cited articles

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error