1887

Abstract

A polychlorophenol-degrading strain, designated MT1, and three MT1-like strains, MT101, MT103 and MT104, were isolated from a cold (4–8 °C) fluidized-bed process treating chlorophenol-contaminated groundwater in southern Finland. The organisms were Gram-negative, rod-shaped, catalase-positive, non-spore-forming and non-motile. Phylogenetic analysis based on 16S rRNA gene sequences indicated that the strains belonged to the -4 subclass of the and were members of the genus . The highest 16S rRNA gene sequence similarity observed for these strains was 96·5 % with the type strains of , and . Chemotaxonomic data (major ubiquinone: Q-10; major polyamine: spermidine; major polar lipids: phosphatidylethanolamine, phosphatidylglycerol, diphosphatidylglycerol, phosphatidylcholine and sphingoglycolipid; major fatty acids: 18 : 17, 16 : 17 and 2-OH 14 : 0) as well as the ability to reduce nitrate supported the affiliation of the strains to the genus . Based on the phylogenetic analysis, whole-cell fatty acid composition as well as biochemical and physiological characteristics, the MT1-like strains were highly similar and could be separated from all recognized species. The novel species sp. nov. is proposed to accommodate strains MT1 (=DSM 13663=CCUG 45847), MT101 (=CCUG 45849), MT103 (=CCUG 45850) and MT104 (=CCUG 45851).

Loading

Article metrics loading...

/content/journal/ijsem/10.1099/ijs.0.63386-0
2005-03-01
2019-10-14
Loading full text...

Full text loading...

/deliver/fulltext/ijsem/55/2/ijs550583.html?itemId=/content/journal/ijsem/10.1099/ijs.0.63386-0&mimeType=html&fmt=ahah

References

  1. Altenburger, P., Kämpfer, P., Makristathis, A., Lubitz, W. & Busse, H.-J. ( 1996; ). Classification of bacteria isolated from a medieval wall painting. J Biotechnol 47, 39–52.[CrossRef]
    [Google Scholar]
  2. Balkwill, D. L., Drake, G. R., Reeves, R. H. & 7 other authors ( 1997; ). Taxonomic study of aromatic-degrading bacteria from deep-terrestrial-subsurface sediments and description of Sphingomonas aromaticivorans sp. nov., Sphingomonas subterranea sp. nov., and Sphingomonas stygia sp. nov. Int J Syst Bacteriol 47, 191–201.[CrossRef]
    [Google Scholar]
  3. Busse, H. J. & Auling, G. ( 1988; ). Polyamine pattern as a chemotaxonomic marker within the Proteobacteria. Syst Appl Microbiol 11, 1–8.[CrossRef]
    [Google Scholar]
  4. Busse, H.-J., Bunka, S., Hensel, A. & Lubitz, W. ( 1997; ). Discrimination of members of the family Pasteurellaceae based on polyamine patterns. Int J Syst Bacteriol 47, 698–708.[CrossRef]
    [Google Scholar]
  5. Busse, H.-J., Kämpfer, P. & Denner, E. B. M. ( 1999; ). Chemotaxonomic characterisation of Sphingomonas. J Ind Microbiol Biotechnol 23, 242–251.[CrossRef]
    [Google Scholar]
  6. Frerichs, G. N. ( 1984; ). The Isolation and Identification of Fish Bacterial Pathogens. Stirling, UK: The Institute of Aquaculture, University of Stirling.
  7. Fujii, K., Satomi, M., Morita, N., Motomura, T., Tanaka, T. & Kikuchi, S. ( 2003; ). Novosphingobium tardaugens sp. nov., an oestradiol-degrading bacterium isolated from activated sludge of a sewage treatment plant in Tokyo. Int J Syst Evol Microbiol 53, 47–52.[CrossRef]
    [Google Scholar]
  8. Galtier, N., Gouy, M. & Gautier, C. ( 1996; ). seaview and phylo_win: two graphic tools for sequence alignment and molecular phylogeny. Comput Appl Biosci 12, 543–548.
    [Google Scholar]
  9. Kämpfer, P., Denner, E. B. M., Meyer, S., Moore, E. R. M. & Busse, H.-J. ( 1997; ). Classification of “Pseudomonas azotocolligans” Anderson 1955, 132, in the genus Sphingomonas as Sphingomonas trueperi sp. nov. Int J Syst Bacteriol 47, 577–583.[CrossRef]
    [Google Scholar]
  10. Kämpfer, P., Witzenberger, R., Denner, E. B. M., Busse, H.-J. & Neef, A. ( 2002; ). Novosphingobium hassiacum sp. nov., a new species isolated from an aerated sewage pond. Syst Appl Microbiol 25, 37–45.[CrossRef]
    [Google Scholar]
  11. Leifson, E. ( 1962; ). The bacterial flora of distilled and stored water. III. New species of the genera Corynebacterium, Flavobacterium, Spirillum, and Pseudomonas. Int Bull Bacteriol Nomencl Taxon 12, 161–170.
    [Google Scholar]
  12. Männistö, M. K. & Puhakka, J. A. ( 2002; ). Psychrophilic and microaerophilic bacteria in boreal groundwater. FEMS Microbiol Ecol 41, 9–16.[CrossRef]
    [Google Scholar]
  13. Männistö, M. K., Tiirola, M. A. & Puhakka, J. A. ( 2001; ). Effects of environmental conditions on 2,3,4,6-tetrachlorophenol degradation by contaminated groundwater and bioreactor bacteria. Biodegradation 12, 291–301.[CrossRef]
    [Google Scholar]
  14. Mesbah, M., Premachandran, U. & Whitman, W. B. ( 1989; ). Precise measurement of the G+C content of deoxyribonucleic acid by high-performance liquid chromatography. Int J Syst Bacteriol 39, 159–167.[CrossRef]
    [Google Scholar]
  15. Nohynek, L. J., Nurmiaho-Lassila, E. L., Suhonen, E. L., Busse, H.-J., Mohammadi, M., Hantula, J., Rainey, F. & Salkinoja-Salonen, M. S. ( 1996; ). Description of chlorophenol-degrading Pseudomonas sp. strains KF1T, KF3, and NKF1 as a new species of the genus Sphingomonas, Sphingomonas subarctica sp. nov. Int J Syst Bacteriol 46, 1042–1055.[CrossRef]
    [Google Scholar]
  16. Reasoner, D. J. & Geldreich, E. E. ( 1985; ). A new medium for the enumeration and subculture of bacteria from potable water. Appl Environ Microbiol 49, 1–7.
    [Google Scholar]
  17. Sohn, J. H., Kwon, K. K., Kang, J.-H., Jung, H.-B. & Kim, S.-J. ( 2004; ). Novosphingobium pentaromaticivorans sp. nov., a high-molecular-mass polycyclic aromatic hydrocarbon-degrading bacterium isolated from estuarine sediment. Int J Syst Evol Microbiol 54, 1483–1487.[CrossRef]
    [Google Scholar]
  18. Takeuchi, M., Sawada, H., Oyaizu, H. & Yokota, A. ( 1994; ). Phylogenetic evidence for Sphingomonas and Rhizomonas as nonphotosynthetic members of the alpha-4 subclass of the Proteobacteria. Int J Syst Bacteriol 44, 308–314.[CrossRef]
    [Google Scholar]
  19. Takeuchi, M., Sakane, T., Yanagi, M., Yamasato, K., Hamana, K. & Yokota, A. ( 1995; ). Taxonomic study of bacteria isolated from plants: proposal of Sphingomonas rosa sp. nov., Sphingomonas pruni sp. nov., Sphingomonas asaccharolytica sp. nov. and Sphingomonas mali sp. nov. Int J Syst Bacteriol 45, 334–341.[CrossRef]
    [Google Scholar]
  20. Takeuchi, M., Hamana, K. & Hiraishi, A. ( 2001; ). Proposal of the genus Sphingomonas sensu stricto and three new genera, Sphingobium, Novosphingobium and Sphingopyxis, on the basis of phylogenetic and chemotaxonomic analyses. Int J Syst Evol Microbiol 51, 1405–1417.
    [Google Scholar]
  21. Thompson, J. D., Higgins, D. G. & Gibson, T. J. ( 1994; ). clustal w: improving the sensitivity of progressive multiple sequence alignment through sequence weighting, position-specific gap penalties and weight matrix choice. Nucleic Acids Res 22, 4673–4680.[CrossRef]
    [Google Scholar]
  22. Tiirola, M. A., Männistö, M. K., Puhakka, J. A. & Kulomaa, M. S. ( 2002; ). Isolation and characterization of Novosphingobium sp. strain MT1, a dominant polychlorophenol-degrading strain in a groundwater bioremediation system. Appl Environ Microbiol 68, 173–180.[CrossRef]
    [Google Scholar]
  23. Tindall, B. J. ( 1990a; ). Lipid composition of Halobacterium lacusprofundi. FEMS Microbiol Lett 66, 199–202.[CrossRef]
    [Google Scholar]
  24. Tindall, B. J. ( 1990b; ). A comparative study of the lipid composition of Halobacterium saccharovorum from various sources. Syst Appl Microbiol 13, 128–130.[CrossRef]
    [Google Scholar]
  25. Väisänen, O., Nurmiaho-Lassila, E.-L., Marmo, S. & Salkinoja-Salonen, M. ( 1994; ). Structure and composition of biological slimes of paper and board machines. Appl Environ Microbiol 60, 641–653.
    [Google Scholar]
  26. Yabuuchi, E., Yano, I., Oyaizu, H., Hashimoto, Y., Ezaki, T. & Yamamoto, H. ( 1990; ). Proposals of Sphingomonas paucimobilis gen. nov. and comb. nov., Sphingomonas parapaucimobilis sp. nov., Sphingomonas yanoikuyae sp. nov., Sphingomonas adhaesiva sp. nov., Sphingomonas capsulata comb. nov., and two genospecies of the genus Sphingomonas. Microbiol Immunol 34, 99–119.[CrossRef]
    [Google Scholar]
  27. Yabuuchi, E., Kosako, Y., Fujiwara, N., Naka, T., Matsunaga, I., Ogura, H. & Kobayashi, K. ( 2002; ). Emendation of the genus Sphingomonas Yabuuchi et al. 1990 and junior objective synonymy of the species of three genera, Sphingobium, Novosphingobium and Sphingopyxis, in conjunction with Blastomonas ursincola. Int J Syst Evol Microbiol 52, 1485–1496.[CrossRef]
    [Google Scholar]
  28. Yurkov, V., Stackebrandt, E., Buss, O., Vermeglio, A., Gorlenko, V. & Beatty, J. T. ( 1997; ). Reorganization of the genus Erythromicrobium: description of Erythromicrobium sibiricum as Sandaracinobacter sibiricus gen. nov., sp. nov., and of Erythromicrobium ursincola as Erythromonas ursincola gen. nov., sp. nov. Int J Syst Bacteriol 47, 1172–1178.[CrossRef]
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/ijsem/10.1099/ijs.0.63386-0
Loading
/content/journal/ijsem/10.1099/ijs.0.63386-0
Loading

Data & Media loading...

Phylogenetic trees calculated using the neighbour-joining method (A), maximum-parsimony method (B) and maximum-likelihood method (C) indicating phylogenetic relationships between strain MT1 (SSP303009) and other members of the genus . The trees are based on a 1375 bp alignment of 16S rRNA gene sequences. The type strains of , and were chosen as outgroups. Bootstrap percentages based on 1000 replications are indicated. Scale bars in (A) and (C) indicate 1 substitution per 100 nucleotides. [PDF of Figs A-C](23 KB)

PDF

Polar lipid profile of MT1 after two-dimensional TLC and detection with molybdatophosphoric acid. Abbreviations: PE, phosphatidylethanolamine; PG, phosphatidylglycerol; DPG, diphosphatidylglycerol; PC, phosphatidylcholine; SGL, sphingoglycolipid; PME, phosphatidylmonomethylethanolamine; PDE, phosphatidyldimethylethanolamine; PLx, unknown phospholipid; PGLx, unknown phosphoglycolipid; GLx, unknown glycolipid; Lx, unknown polar lipid. [PDF of Figs D and E](114 KB)

PDF

Whole-cell fatty acid composition of the members of the genus and strains MT1 , MT101, MT103 and MT104 grown in R2A broth at 22 °C. [PDF](18 KB)

PDF

Most Cited This Month

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error