1887

INTERNATIONAL JOURNAL OF SYSTEMATIC AND EVOLUTIONARY MICROBIOLOGY logo

Volume 55, Issue 2

sp. nov., a psychrotolerant bacterium from a polychlorophenol bioremediation process Free

Abstract

A polychlorophenol-degrading strain, designated MT1, and three MT1-like strains, MT101, MT103 and MT104, were isolated from a cold (4–8 °C) fluidized-bed process treating chlorophenol-contaminated groundwater in southern Finland. The organisms were Gram-negative, rod-shaped, catalase-positive, non-spore-forming and non-motile. Phylogenetic analysis based on 16S rRNA gene sequences indicated that the strains belonged to the -4 subclass of the and were members of the genus . The highest 16S rRNA gene sequence similarity observed for these strains was 96·5 % with the type strains of , and . Chemotaxonomic data (major ubiquinone: Q-10; major polyamine: spermidine; major polar lipids: phosphatidylethanolamine, phosphatidylglycerol, diphosphatidylglycerol, phosphatidylcholine and sphingoglycolipid; major fatty acids: 18 : 17, 16 : 17 and 2-OH 14 : 0) as well as the ability to reduce nitrate supported the affiliation of the strains to the genus . Based on the phylogenetic analysis, whole-cell fatty acid composition as well as biochemical and physiological characteristics, the MT1-like strains were highly similar and could be separated from all recognized species. The novel species sp. nov. is proposed to accommodate strains MT1 (=DSM 13663=CCUG 45847), MT101 (=CCUG 45849), MT103 (=CCUG 45850) and MT104 (=CCUG 45851).

  • Published Online:
Keyword(s): FAME, fatty acid methyl ester
SGM
Loading

Article metrics loading...

/content/journal/ijsem/10.1099/ijs.0.63386-0
2005-03-01
2024-04-20
Loading full text...

Full text loading...

/deliver/fulltext/ijsem/55/2/ijs550583.html?itemId=/content/journal/ijsem/10.1099/ijs.0.63386-0&mimeType=html&fmt=ahah

References

  1. Altenburger P., Kämpfer P., Makristathis A., Lubitz W., Busse H.-J. 1996; Classification of bacteria isolated from a medieval wall painting. J Biotechnol 47:39–52 [CrossRef]
     [Google Scholar]
  2. Balkwill D. L., Drake G. R., Reeves R. H. 7 other authors 1997; Taxonomic study of aromatic-degrading bacteria from deep-terrestrial-subsurface sediments and description of Sphingomonas aromaticivorans sp. nov., Sphingomonas subterranea sp. nov., and Sphingomonas stygia sp. nov. Int J Syst Bacteriol 47:191–201 [CrossRef]
     [Google Scholar]
  3. Busse H. J., Auling G. 1988; Polyamine pattern as a chemotaxonomic marker within the Proteobacteria . Syst Appl Microbiol 11:1–8 [CrossRef]
     [Google Scholar]
  4. Busse H.-J., Bunka S., Hensel A., Lubitz W. 1997; Discrimination of members of the family Pasteurellaceae based on polyamine patterns. Int J Syst Bacteriol 47:698–708 [CrossRef]
     [Google Scholar]
  5. Busse H.-J., Kämpfer P., Denner E. B. M. 1999; Chemotaxonomic characterisation of Sphingomonas . J Ind Microbiol Biotechnol 23:242–251 [CrossRef]
     [Google Scholar]
  6. Frerichs G. N. 1984 The Isolation and Identification of Fish Bacterial Pathogens Stirling, UK: The Institute of Aquaculture, University of Stirling;
     [Google Scholar]
  7. Fujii K., Satomi M., Morita N., Motomura T., Tanaka T., Kikuchi S. 2003; Novosphingobium tardaugens sp. nov., an oestradiol-degrading bacterium isolated from activated sludge of a sewage treatment plant in Tokyo. Int J Syst Evol Microbiol 53:47–52 [CrossRef]
     [Google Scholar]
  8. Galtier N., Gouy M., Gautier C. 1996; seaview and phylo_win: two graphic tools for sequence alignment and molecular phylogeny. Comput Appl Biosci 12:543–548
     [Google Scholar]
  9. Kämpfer P., Denner E. B. M., Meyer S., Moore E. R. M., Busse H.-J. 1997; Classification of “ Pseudomonas azotocolligans ” Anderson 1955, 132, in the genus Sphingomonas as Sphingomonas trueperi sp. nov. Int J Syst Bacteriol 47:577–583 [CrossRef]
     [Google Scholar]
  10. Kämpfer P., Witzenberger R., Denner E. B. M., Busse H.-J., Neef A. 2002; Novosphingobium hassiacum sp. nov., a new species isolated from an aerated sewage pond. Syst Appl Microbiol 25:37–45 [CrossRef]
     [Google Scholar]
  11. Leifson E. 1962; The bacterial flora of distilled and stored water. III. New species of the genera Corynebacterium , Flavobacterium , Spirillum , and Pseudomonas . Int Bull Bacteriol Nomencl Taxon 12:161–170
     [Google Scholar]
  12. Männistö M. K., Puhakka J. A. 2002; Psychrophilic and microaerophilic bacteria in boreal groundwater. FEMS Microbiol Ecol 41:9–16 [CrossRef]
     [Google Scholar]
  13. Männistö M. K., Tiirola M. A., Puhakka J. A. 2001; Effects of environmental conditions on 2,3,4,6-tetrachlorophenol degradation by contaminated groundwater and bioreactor bacteria. Biodegradation 12:291–301 [CrossRef]
     [Google Scholar]
  14. Mesbah M., Premachandran U., Whitman W. B. 1989; Precise measurement of the G+C content of deoxyribonucleic acid by high-performance liquid chromatography. Int J Syst Bacteriol 39:159–167 [CrossRef]
     [Google Scholar]
  15. Nohynek L. J., Nurmiaho-Lassila E. L., Suhonen E. L., Busse H.-J., Mohammadi M., Hantula J., Rainey F., Salkinoja-Salonen M. S. 1996; Description of chlorophenol-degrading Pseudomonas sp. strains KF1T, KF3, and NKF1 as a new species of the genus Sphingomonas , Sphingomonas subarctica sp. nov. Int J Syst Bacteriol 46:1042–1055 [CrossRef]
     [Google Scholar]
  16. Reasoner D. J., Geldreich E. E. 1985; A new medium for the enumeration and subculture of bacteria from potable water. Appl Environ Microbiol 49:1–7
     [Google Scholar]
  17. Sohn J. H., Kwon K. K., Kang J.-H., Jung H.-B., Kim S.-J. 2004; Novosphingobium pentaromaticivorans sp. nov., a high-molecular-mass polycyclic aromatic hydrocarbon-degrading bacterium isolated from estuarine sediment. Int J Syst Evol Microbiol 54:1483–1487 [CrossRef]
     [Google Scholar]
  18. Takeuchi M., Sawada H., Oyaizu H., Yokota A. 1994; Phylogenetic evidence for Sphingomonas and Rhizomonas as nonphotosynthetic members of the alpha-4 subclass of the Proteobacteria . Int J Syst Bacteriol 44:308–314 [CrossRef]
     [Google Scholar]
  19. Takeuchi M., Sakane T., Yanagi M., Yamasato K., Hamana K., Yokota A. 1995; Taxonomic study of bacteria isolated from plants: proposal of Sphingomonas rosa sp. nov., Sphingomonas pruni sp. nov., Sphingomonas asaccharolytica sp.nov. and Sphingomonas mali sp. nov. Int J Syst Bacteriol 45:334–341 [CrossRef]
     [Google Scholar]
  20. Takeuchi M., Hamana K., Hiraishi A. 2001; Proposal of the genus Sphingomonas sensu stricto and three new genera,Sphingobium , Novosphingobium and Sphingopyxis , on the basis of phylogenetic and chemotaxonomic analyses. Int J Syst Evol Microbiol 51:1405–1417
     [Google Scholar]
  21. Thompson J. D., Higgins D. G., Gibson T. J. 1994; clustal w: improving the sensitivity of progressive multiple sequence alignment through sequence weighting, position-specific gap penalties and weight matrix choice. Nucleic Acids Res 22:4673–4680 [CrossRef]
     [Google Scholar]
  22. Tiirola M. A., Männistö M. K., Puhakka J. A., Kulomaa M. S. 2002; Isolation and characterization of Novosphingobium sp. strain MT1, a dominant polychlorophenol-degrading strain in a groundwater bioremediation system. Appl Environ Microbiol 68:173–180 [CrossRef]
     [Google Scholar]
  23. Tindall B. J. 1990a; Lipid composition of Halobacterium lacusprofundi . FEMS Microbiol Lett 66:199–202 [CrossRef]
     [Google Scholar]
  24. Tindall B. J. 1990b; A comparative study of the lipid composition of Halobacterium saccharovorum from various sources. Syst Appl Microbiol 13:128–130 [CrossRef]
     [Google Scholar]
  25. Väisänen O., Nurmiaho-Lassila E.-L., Marmo S., Salkinoja-Salonen M. 1994; Structure and composition of biological slimes of paper and board machines. Appl Environ Microbiol 60:641–653
     [Google Scholar]
  26. Yabuuchi E., Yano I., Oyaizu H., Hashimoto Y., Ezaki T., Yamamoto H. 1990; Proposals of Sphingomonas paucimobilis gen.nov. and comb. nov., Sphingomonas parapaucimobilis sp. nov., Sphingomonas yanoikuyae sp. nov.,Sphingomonas adhaesiva sp. nov., Sphingomonascapsulata comb. nov., and two genospecies of the genus Sphingomonas . Microbiol Immunol 34:99–119 [CrossRef]
     [Google Scholar]
  27. Yabuuchi E., Kosako Y., Fujiwara N., Naka T., Matsunaga I., Ogura H., Kobayashi K. 2002; Emendation of the genus Sphingomonas Yabuuchi et al . 1990 and junior objective synonymy of the species of three genera, Sphingobium , Novosphingobium and Sphingopyxis , in conjunction with Blastomonas ursincola . Int J Syst Evol Microbiol 52:1485–1496 [CrossRef]
     [Google Scholar]
  28. Yurkov V., Stackebrandt E., Buss O., Vermeglio A., Gorlenko V., Beatty J. T. 1997; Reorganization of the genus Erythromicrobium : description of Erythromicrobium sibiricum as Sandaracinobacter sibiricus gen.nov., sp. nov., and of Erythromicrobium ursincola as Erythromonas ursincola gen. nov., sp. nov. Int J Syst Bacteriol 47:1172–1178 [CrossRef]
     [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/ijsem/10.1099/ijs.0.63386-0
Loading
Novosphingobium lentum sp. nov., a psychrotolerant bacterium from a polychlorophenol bioremediation process
Int J Syst Evol Microbiol 55, 583 (2005); https://doi.org/10.1099/ijs.0.63386-0
/content/journal/ijsem/10.1099/ijs.0.63386-0
/content/journal/ijsem/10.1099/ijs.0.63386-0
Loading

Data & Media loading...

Supplements

Supplementary material 1

PDF

Supplementary material 2

PDF

Supplementary material 3

PDF

Most cited Most Cited RSS feed

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error