1887

Abstract

A polychlorophenol-degrading strain, designated MT1, and three MT1-like strains, MT101, MT103 and MT104, were isolated from a cold (4–8 °C) fluidized-bed process treating chlorophenol-contaminated groundwater in southern Finland. The organisms were Gram-negative, rod-shaped, catalase-positive, non-spore-forming and non-motile. Phylogenetic analysis based on 16S rRNA gene sequences indicated that the strains belonged to the -4 subclass of the and were members of the genus . The highest 16S rRNA gene sequence similarity observed for these strains was 96·5 % with the type strains of , and . Chemotaxonomic data (major ubiquinone: Q-10; major polyamine: spermidine; major polar lipids: phosphatidylethanolamine, phosphatidylglycerol, diphosphatidylglycerol, phosphatidylcholine and sphingoglycolipid; major fatty acids: 18 : 17, 16 : 17 and 2-OH 14 : 0) as well as the ability to reduce nitrate supported the affiliation of the strains to the genus . Based on the phylogenetic analysis, whole-cell fatty acid composition as well as biochemical and physiological characteristics, the MT1-like strains were highly similar and could be separated from all recognized species. The novel species sp. nov. is proposed to accommodate strains MT1 (=DSM 13663=CCUG 45847), MT101 (=CCUG 45849), MT103 (=CCUG 45850) and MT104 (=CCUG 45851).

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2005-03-01
2021-04-21
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