1887

Abstract

A Gram-positive, aerobic, non-motile actinomycete, strain GW 14-1925, that formed branched substrate and aerial mycelium was studied using a polyphasic approach. On the basis of 16S rRNA gene sequence similarity studies, strain GW 14-1925 was shown to belong to the genus , being most closely related to (97·9 %), (98·9 %), (98·6 %), (98·5 %), (98·3 %), subsp. (98·1 %) and subsp. (97·7 %). The 16S rRNA gene sequence similarity to other species was <97·5 %. Chemotaxonomic data [major menaquinones of the MK-9 series with minor amounts of MK-8(H); major polar lipids of phospholipid type IV; fatty acids with major amounts of iso- and anteiso- and 10-methyl-branched fatty acids in combination with iso-branched 2-hydroxy fatty acids] supported allocation of the strain to the genus . The results of DNA–DNA hybridizations and physiological and biochemical tests allowed genotypic and phenotypic differentiation of strain GW 14-1925 from closely related species; thus, GW 14-1925 represents a novel species of the genus , for which the name sp. nov. is proposed, with GW 14-1925 (=DSM 44753=NRRL B-24325) as the type strain.

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2005-03-01
2019-10-18
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