Full text loading...
Abstract
A Gram-positive, aerobic, non-motile actinomycete, strain GW 14-1925T, that formed branched substrate and aerial mycelium was studied using a polyphasic approach. On the basis of 16S rRNA gene sequence similarity studies, strain GW 14-1925T was shown to belong to the genus Nonomuraea, being most closely related to Nonomuraea longicatena (97·9 %), Nonomuraea turkmeniaca (98·9 %), Nonomuraea helvata (98·6 %), Nonomuraea polychroma (98·5 %), Nonomuraea salmonaea (98·3 %), Nonomuraea roseoviolacea subsp. roseoviolacea (98·1 %) and Nonomuraea roseoviolacea subsp. carminata (97·7 %). The 16S rRNA gene sequence similarity to other Nonomuraea species was <97·5 %. Chemotaxonomic data [major menaquinones of the MK-9 series with minor amounts of MK-8(H4); major polar lipids of phospholipid type IV; fatty acids with major amounts of iso- and anteiso- and 10-methyl-branched fatty acids in combination with iso-branched 2-hydroxy fatty acids] supported allocation of the strain to the genus Nonomuraea. The results of DNA–DNA hybridizations and physiological and biochemical tests allowed genotypic and phenotypic differentiation of strain GW 14-1925T from closely related species; thus, GW 14-1925T represents a novel species of the genus Nonomuraea, for which the name Nonomuraea kuesteri sp. nov. is proposed, with GW 14-1925T (=DSM 44753T=NRRL B-24325T) as the type strain.
- Published Online: