1887

Abstract

Three strains isolated from the soil of a garden in Tokyo, Japan, were characterized physiologically, biochemically and in terms of fatty acid profile, DNA–DNA relatedness and 16S rRNA gene sequence. The isolates were Gram-negative, aerobic, rod-shaped cells with polar flagellation. According to DNA–DNA similarity, the strains belonged to the same species. The bacteria grew at temperatures from 10 to 37 °C, with an optimum around 25–30 °C. Growth was observed at pH values from 5·6 to 8·0. The DNA G+C content ranged from 63·4 to 64·0 mol%. Phylogenetic analyses of 16S rRNA gene sequences revealed a clear affiliation with members of the family ‘’. The closest relationship was seen with and , but, in terms of physiology and fatty acid profile, the bacteria described were rather distant from and . On the basis of phenotypic and phylogenetic distinctness, it is proposed that the isolates represent a novel species in a novel genus, namely gen. nov., sp. nov. The type strain is XD53 (=IAM 15069=DSM 16301=ATCC BAA-939).

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2005-03-01
2020-09-19
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