1887

INTERNATIONAL JOURNAL OF SYSTEMATIC AND EVOLUTIONARY MICROBIOLOGY logo

Volume 55, Issue 1

sp. nov., from a human dental abscess Free

Abstract

A previously undescribed filamentous, beaded, Gram-positive, rod-shaped bacterium was isolated from pus of a human dental abscess. Based on its cellular morphology and the results of biochemical testing the organism was tentatively identified as a member of the genus , but it did not correspond to any currently recognized species of this genus. Comparative 16S rRNA gene sequencing studies showed the bacterium represents a distinct subline within the genus , clustering within a group of species that includes , the type species of the genus. Sequence divergence values of >8 % with other recognized species within this phylogenetic group clearly demonstrated that the organism represents a hitherto unknown species. Based on biochemical and molecular phylogenetic evidence, it is proposed that the unidentified organism recovered from a dental abscess be classified as a novel species, sp. nov. The type strain is R18165 (=CCUG 48064=CIP 108337).

  • Published Online:
SGM
Loading

Article metrics loading...

/content/journal/ijsem/10.1099/ijs.0.63376-0
2005-01-01
2024-04-25
Loading full text...

Full text loading...

/deliver/fulltext/ijsem/55/1/ijs550427.html?itemId=/content/journal/ijsem/10.1099/ijs.0.63376-0&mimeType=html&fmt=ahah

References

  1. Collins M. D., Hoyles L., Kalfas S., Sundquist G., Monsen T., Nikolaitchouk N., Falsen E. 2000; Characterisation of Actinomyces isolates from infected root canals of teeth: description of Actinomyces radicidentis sp. nov. J Clin Microbiol 38:3399–3403
     [Google Scholar]
  2. Funke G., Stubbs S., von Graevenitz A., Collins M. D. 1994; Assignment of human-derived CDC group 1 coryneform bacteria and CDC group 1-like coryneform bacteria to the genus Actinomyces as Actinomyces neuii subsp. neuii sp. nov., subsp.nov., and Actinomyces neuii subsp. anitratus subsp. nov. Int J Syst Bacteriol 44:167–171 [CrossRef]
     [Google Scholar]
  3. Funke G., Von Graevenitz A., Clarridge J. E. III, Bernard K. A. 1997a; Clinical microbiology of coryneform bacteria. Clin Microbiol Rev 10:125–159
     [Google Scholar]
  4. Funke G., Alvarez N., Pascual C., Falsen E., Akervall E., Sabbe L., Schouls L., Weiss N., Collins M. D. 1997b; Actinomyces europaeus sp. nov., isolated from human clinical specimens. Int J Syst Bacteriol 47:687–692 [CrossRef]
     [Google Scholar]
  5. Hall V., O'Neill G. L., Magee J. T., Duerden B. I. 1999; Development of amplified 16S ribosomal DNA restriction analysis for identification of Actinomyces species and comparison with pyrolysis-mass spectrometry and conventional biochemical tests. J Clin Microbiol 37:2255–2261
     [Google Scholar]
  6. Hall V., Talbot P. R., Stubbs S. L., Duerden B. I. 2001; Identification of clinical isolates of Actinomyces species by amplified 16S ribosomal DNA restriction analysis (ARDRA. J Clin Microbiol 39:3555–3562 [CrossRef]
     [Google Scholar]
  7. Hall V., Collins M. D., Hutson R., Falsen E., Duerden B. I. 2002; Actinomyces cardiffensis sp. nov. from human clinical sources. J Clin Microbiol 40:3427–3431 [CrossRef]
     [Google Scholar]
  8. Holdeman L. V., Cato E. P., Moore W. E. C. 1977 Anaerobe Laboratory Manual , 4th edn. Blacksburg, VA: Virginia Polytechnic Institute and State University;
     [Google Scholar]
  9. Lawson P. A., Nikolaitchouk N., Falsen E., Westling K., Collins M. D. 2001; Actinomyces funkei sp. nov., isolated from human clinical specimens. Int J Syst Evol Microbiol 51:853–855 [CrossRef]
     [Google Scholar]
  10. Mesbah M., Premachandran U., Whitman W. B. 1989; Precise measurement of the G+C content of deoxyribonucleic acid by high-performance liquid chromatography. Int J Syst Bacteriol 39:159–167 [CrossRef]
     [Google Scholar]
  11. Nicholas K. B., Nicholas H. B. Jr, Deerfield D. W. II 1997; genedoc: analysis and visualisation of genetic variation. EMBNEW News 4:14
     [Google Scholar]
  12. Nikolaitchouk N., Hoyles L., Falsen E., Grainger J. M., Collins M. D. 2000; Characterisation of Actinomyces isolates from samples from human urogenital tract: description of Actinomyces urogenitalis sp. nov. Int J Syst Evol Microbiol 50:1649–1654 [CrossRef]
     [Google Scholar]
  13. Page R. D. M. 1996; treeview: an application to display phylogenetic trees on personal computers. Comput Appl Biosci 12:357–358
     [Google Scholar]
  14. Pascual C., Falsen E., Akervall E., Sjoden B., Collins M. D. 1997; Actinomyces graevenitzii sp. nov., isolated from human clinical specimens. Int J Syst Bacteriol 47:885–888 [CrossRef]
     [Google Scholar]
  15. Phillips K. D. 1976; A simple and sensitive technique for determining the fermentation reactions of non-sporing anaerobes. J Appl Bacteriol 41:325–328 [CrossRef]
     [Google Scholar]
  16. Rasmussen S. W. 2002; SEQtools, a software package for analysis of nucleotide and protein sequences. Available at http://www.seqtools.dk
  17. Schaal K. P. 1998; Actinomycoses, actinobacillosis and related diseases. In Topley and Wilson's Microbiology and Microbial Infections , 9th edn.vol 3 pp  777–798 Edited by Hausler W. J., Sussman M. London: Arnold;
     [Google Scholar]
  18. Stackebrandt E., Goebel B. M. 1994; Taxonomic note: a place for DNA–DNA reassociation and 16S rRNA sequence analysis in the present species definition in bacteriology. Int J Syst Bacteriol 44:846–849 [CrossRef]
     [Google Scholar]
  19. Wüst J., Stubbs S., Weiss N., Funke G., Collins M. D. 1995; Assignment of Actinomyces pyogenes -like (CDC coryneform group E) bacteria to the genus Actinomyces as Actinomyces radingae sp.nov. and Actinomyces turicensis sp. nov. Lett Appl Microbiol 20:76–81 [CrossRef]
     [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/ijsem/10.1099/ijs.0.63376-0
Loading
Actinomyces dentalis sp. nov., from a human dental abscess
Int J Syst Evol Microbiol 55, 427 (2005); https://doi.org/10.1099/ijs.0.63376-0
/content/journal/ijsem/10.1099/ijs.0.63376-0
/content/journal/ijsem/10.1099/ijs.0.63376-0
Loading

Data & Media loading...

Most cited Most Cited RSS feed

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error