1887

Abstract

Four Gram-positive, rod- or coccus-shaped bacterial strains, KSL-1, KSL-9, KSL-10 and KSL-12, were isolated from an alkaline serpentinite soil in Korea, and their taxonomic positions were investigated in a polyphasic study. The four strains exhibited no difference in their 16S rRNA gene sequences. Phylogenetic analyses based on 16S rRNA gene sequences showed that the four strains were phylogenetically affiliated to the genus . The four strains had cell-wall peptidoglycan based on -diaminopimelic acid as the diamino acid, indicating wall chemotype I. The predominant menaquinone detected in the four strains was MK-8(H). The major fatty acid components were iso-C, 10-methyl-C, C 9 and C 6. The DNA G+C contents were 72·4–73·6 mol%. The four strains exhibited 16S rRNA gene sequence similarity levels of 94·0–96·3 % to the type strains of species with validly published names. DNA–DNA relatedness levels between the four strains were 85–91 %. On the basis of phenotypic properties, phylogenetic distinctiveness and genotypic relatedness, strains KSL-1, KSL-9, KSL-10 and KSL-12 were classified in the genus as members of a novel species, sp. nov. The type strain is strain KSL-1 (=KCTC 19037=DSM 16699).

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2005-03-01
2019-12-06
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References

  1. Collins, M. D., Dorsch, M. & Stackebrandt, E. ( 1989; ). Transfer of Pimelobacter tumescens to Terrabacter gen. nov. as Terrabacter tumescens comb. nov. and of Pimelobacter jensenii to Nocardioides as Nocardioides jensenii comb. nov. Int J Syst Bacteriol 39, 1–6.[CrossRef]
    [Google Scholar]
  2. Collins, M. D., Cockcroft, S. & Wallbanks, S. ( 1994; ). Phylogenetic analysis of a new ll-diaminopimelic acid-containing coryneform bacterium from herbage, Nocardioides plantarum sp. nov. Int J Syst Bacteriol 44, 523–526.[CrossRef]
    [Google Scholar]
  3. Cowan, S. T. & Steel, K. J. ( 1965; ). Manual for the Identification of Medical Bacteria. London: Cambridge University Press.
  4. Ezaki, T., Hashimoto, Y. & Yabuuchi, E. ( 1989; ). Fluorometric deoxyribonucleic acid-deoxyribonucleic acid hybridization in microdilution wells as an alternative to membrane filter hybridization in which radioisotopes are used to determine genetic relatedness among bacterial strains. Int J Syst Bacteriol 39, 224–229.[CrossRef]
    [Google Scholar]
  5. Komagata, K. & Suzuki, K. ( 1987; ). Lipids and cell-wall analysis in bacterial systematics. Methods Microbiol 19, 161–203.
    [Google Scholar]
  6. Lanyi, B. ( 1987; ). Classical and rapid identification methods for medically important bacteria. Methods Microbiol 19, 1–67.
    [Google Scholar]
  7. Lawson, P. A., Collins, M. D., Schumann, P., Tindall, B. J., Hirsch, P. & Labrenz, M. ( 2000; ). New ll-diaminopimelic acid-containing actinomycetes from hypersaline, heliothermal and meromictic Antarctic Ekho Lake: Nocardioides aquaticus sp. nov. and Friedmanniella lacustris sp. nov. Syst Appl Microbiol 23, 219–229.[CrossRef]
    [Google Scholar]
  8. Lechevalier, M. P. & Lechevalier, H. A. ( 1970; ). A critical evaluation of the genera of aerobic actinomycetes. In The Actinomycetales, pp. 393–405. Edited by H. Prauser. Jena: Gustav Fischer Verlag.
  9. Miller, E. S., Woese, C. R. & Brenner, S. ( 1991; ). Description of the erythromycin-producing bacterium Arthrobacter sp. strain NRRL B-3381 as Aeromicrobium erythreum gen. nov., sp. nov. Int J Syst Bacteriol 41, 363–368.[CrossRef]
    [Google Scholar]
  10. O'Donnell, A. G., Goodfellow, M. & Minnikin, D. E. ( 1982; ). Lipids in the classification of Nocardioides: reclassification of Arthrobacter simplex (Jensen) Lochhead in the genus Nocardioides (Prauser) emend. O'Donnell et al. as Nocardioides simplex comb. nov. Arch Microbiol 133, 323–329.[CrossRef]
    [Google Scholar]
  11. Park, Y.-H., Yoon, J.-H., Shin, Y. K., Suzuki, K., Kudo, T., Seino, A., Kim, H.-J., Lee, J.-S. & Lee, S. T. ( 1999; ). Classification of ‘Nocardioides fulvus’ IFO 14399 and Nocardioides sp. ATCC 39419 in Kribbella gen. nov., as Kribbella flavida sp. nov. and Kribbella sandramycini sp. nov. Int J Syst Bacteriol 49, 743–752.[CrossRef]
    [Google Scholar]
  12. Prauser, H. ( 1976; ). Nocardioides, a new genus of the order Actinomycetales. Int J Syst Bacteriol 26, 58–65.[CrossRef]
    [Google Scholar]
  13. Prauser, H. ( 1984; ). Nocardioides luteus spec. nov. Z Allg Microbiol 24, 647–648.[CrossRef]
    [Google Scholar]
  14. Prauser, H. ( 1989; ). Genus Nocardioides Prauser 1976 . In Bergey's Manual of Systematic Bacteriology, vol. 4, pp. 2371–2375. Edited by S. T. Williams, M. E. Sharpe & J. G. Holt. Baltimore: Williams & Wilkins.
  15. Sasser, M. ( 1990; ). Identification of Bacteria by Gas Chromatography of Cellular Fatty Acids. Newark, DE: MIDI.
  16. Shirling, E. B. & Gottlieb, D. ( 1966; ). Methods for characterization of Streptomyces species. Int J Syst Bacteriol 16, 313–340.[CrossRef]
    [Google Scholar]
  17. Stackebrandt, E. & Goebel, B. M. ( 1994; ). Taxonomic note: a place for DNA-DNA reassociation and 16S rRNA sequence analysis in the present species definition in bacteriology. Int J Syst Bacteriol 44, 846–849.[CrossRef]
    [Google Scholar]
  18. Suzuki, K. & Komagata, K. ( 1983; ). Pimelobacter gen. nov., a new genus of coryneform bacteria with ll-diaminopimelic acid in the cell wall. J Gen Appl Microbiol 29, 59–71.[CrossRef]
    [Google Scholar]
  19. Tamaoka, J. & Komagata, K. ( 1984; ). Determination of DNA base composition by reverse-phase high-performance liquid chromatography. FEMS Microbiol Lett 25, 125–128.[CrossRef]
    [Google Scholar]
  20. Tamura, T. & Yokota, A. ( 1994; ). Transfer of Nocardioides fastidiosa Collins and Stackebrandt 1989 to the genus Aeromicrobium as Aeromicrobium fastidiosum comb. nov. Int J Syst Bacteriol 44, 608–611.[CrossRef]
    [Google Scholar]
  21. Urzì, C., Salamone, P., Schumann, P. & Stackebrandt, E. ( 2000; ). Marmoricola aurantiacus gen. nov., sp. nov., a coccoid member of the family Nocardioidaceae isolated from a marble statue. Int J Syst Evol Microbiol 50, 529–536.[CrossRef]
    [Google Scholar]
  22. Wang, Y. M., Zhang, Z. S., Xu, X. L., Ruan, J. S. & Wang, Y. ( 2001; ). Actinopolymorpha singaporensis gen. nov., sp. nov., a novel actinomycete from the tropical rainforest of Singapore. Int J Syst Evol Microbiol 51, 467–473.
    [Google Scholar]
  23. Wayne, L. G., Brenner, D. J., Colwell, R. R. & 9 other authors ( 1987; ). International Committee on Systematic Bacteriology. Report of the ad hoc committee on reconciliation of approaches to bacterial systematics. Int J Syst Bacteriol 37, 463–464.[CrossRef]
    [Google Scholar]
  24. Yi, H. & Chun, J. ( 2004a; ). Nocardioides ganghwensis sp. nov., isolated from tidal flat sediment. Int J Syst Evol Microbiol 54, 1295–1299.[CrossRef]
    [Google Scholar]
  25. Yi, H. & Chun, J. ( 2004b; ). Nocardioides aestuarii sp. nov., isolated from tidal flat sediment. Int J Syst Evol Microbiol 54, 2151–2154.[CrossRef]
    [Google Scholar]
  26. Yoon, J.-H., Kim, H., Kim, S.-B., Kim, H.-J., Kim, W. Y., Lee, S. T., Goodfellow, M. & Park, Y.-H. ( 1996; ). Identification of Saccharomonospora strains by the use of genomic DNA fragments and rRNA gene probes. Int J Syst Bacteriol 46, 502–505.[CrossRef]
    [Google Scholar]
  27. Yoon, J.-H., Rhee, S.-K., Lee, J.-S., Park, Y.-H. & Lee, S. T. ( 1997; ). Nocardioides pyridinolyticus sp. nov., a pyridine-degrading bacterium isolated from the oxic zone of an oil shale column. Int J Syst Bacteriol 47, 933–938.[CrossRef]
    [Google Scholar]
  28. Yoon, J.-H., Lee, S. T. & Park, Y.-H. ( 1998; ). Inter- and intraspecific phylogenetic analysis of the genus Nocardioides and related taxa based on 16S rRNA gene sequences. Int J Syst Bacteriol 48, 187–194.[CrossRef]
    [Google Scholar]
  29. Yoon, J.-H., Cho, Y.-G., Lee, S. T., Suzuki, K.-I., Nakase, T. & Park, Y.-H. ( 1999; ). Nocardioides nitrophenolicus sp. nov., a p-nitrophenol-degrading bacterium. Int J Syst Bacteriol 49, 675–680.[CrossRef]
    [Google Scholar]
  30. Yoon, J.-H., Kim, I.-G., Kang, K. H., Oh, T.-K. & Park, Y.-H. ( 2004; ). Nocardioides aquiterrae sp. nov., isolated from groundwater in Korea. Int J Syst Evol Microbiol 54, 71–75.[CrossRef]
    [Google Scholar]
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vol. , part 2, pp. 809 - 814

Percentage cellular fatty acid compositions of strains KSL-1 , KSL-9, KSL-10 and KSL-12. [PDF](16 KB)



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