@article{mbs:/content/journal/ijsem/10.1099/ijs.0.63372-0, author = "Zhang, Chunyang and Liu, Xiaoli and Dong, Xiuzhu", title = "Syntrophomonas erecta sp. nov., a novel anaerobe that syntrophically degrades short-chain fatty acids", journal= "International Journal of Systematic and Evolutionary Microbiology", year = "2005", volume = "55", number = "2", pages = "799-803", doi = "https://doi.org/10.1099/ijs.0.63372-0", url = "https://www.microbiologyresearch.org/content/journal/ijsem/10.1099/ijs.0.63372-0", publisher = "Microbiology Society", issn = "1466-5034", type = "Journal Article", keywords = "UASB, upflow anaerobic sludge blanket", keywords = "SCFAs, short-chain fatty acids", abstract = "Two novel anaerobes, strains GB4-38T and SB9-1, were isolated from an upflow anaerobic sludge blanket reactor for treating bean-curd farm wastewater and lotus field mud, respectively. The strains degraded straight-chain fatty acids with 4–8 carbon atoms in syntrophic association with methanogens and converted 1 mol butyrate into about 2 mol acetate and presumably 2 mol H2. None of the branched-chain fatty acids tested could be degraded. Benzoate was not degraded. Fumarate, sulfate, thiosulfate, sulfur and nitrate did not serve as electron acceptors for butyrate degradation. In the absence of a methanogen partner, strain GB4-38T grew on crotonate in pure culture; the generation time was about 5 h at 37 °C. However, strain SB9-1 grew on butyrate plus pentenoate, but not crotonate, in pure culture and the generation time was 18 h at 37 °C. Cells of GB4-38T and SB9-1 were straight rods and stained Gram-negative. The major cellular fatty acids of GB4-38T were C14 : 0 (29·74 %), C16 : 0 (17·00 %), C16 : 1 ω5c (16·63 %) and isoC17 : 1 I (15·34 %). ll-Diaminopimelic acid existed in the cellular peptidoglycan. The genomic DNA G+C content of strain GB4-38T was 43·2 mol%. Phylogenetic analysis based on 16S rRNA gene sequences supported clustering of the two strains with syntrophic bacterial species of the genus Syntrophomonas (89·6–92·4 % sequence similarity), but phenotypic, chemotaxonomic and genetic characters differentiated the two strains from members of this genus. Therefore, it is proposed that the two strains are representatives of a novel species, Syntrophomonas erecta sp. nov. The type strain is GB4-38T (=CGMCC 1.5013T=DSM 16215T).", }