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Abstract

Six new -like isolates originating from different species of bleached and healthy corals around Magnetic Island (Australia) were investigated using a polyphasic approach. Phylogenetic analyses based on 16S rRNA, and gene sequences split the isolates in two new groups. Strains LMG 22223, LMG 22224, LMG 22225, LMG 22226 and LMG 22227 were phylogenetic neighbours of LMG 4228 (95·6 % 16S rRNA gene sequence similarity), whereas strain LMG 22228 was related to LMG 19839 (95·5 % 16S rRNA gene sequence similarity). The two new groups can be distinguished from closely related species on the basis of several phenotypic features, including fermentation of -mannitol, melibiose and sucrose, and utilization of different compounds as carbon sources, arginine dihydrolase activity, nitrate reduction, resistance to the vibriostatic agent O/129 and the presence of fatty acids 15 : 0 iso and 17 : 0 iso. The names sp. nov. (type strain LMG 22223=CBMAI 622=CC1) and sp. nov. (type strain LMG 22228=CBMAI 623=CC17) are proposed to accommodate these new isolates. The G+C contents of the DNA of the two type strains are respectively 47·6 and 48·2 mol%.

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2005-03-01
2019-11-22
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vol. , part 2, pp. 913 - 917

Neighbour-joining tree showing the relationships among representative strains on the basis of (928 nt) gene sequences. Bootstrap values after 500 replications are shown. Bar, 1 % estimated sequence divergence.

Neighbour-joining tree showing the relationships among representative strains on the basis of (797 nt) gene sequences. Bootstrap values after 500 replications are shown. Bar, 10 % estimated sequence divergence.

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