1887

Abstract

An actinomycete strain was recovered from a pond where radon is known to be dissolved. A polyphasic study was undertaken to identify the new isolate. The 16S rRNA gene sequence of strain WA201 showed closest similarity to the type strains of (98·5 %) and (98·1 %). The chemotaxonomic results confirmed the taxonomic position of the isolate in the genus . DNA–DNA relatedness values supported the classification of this isolate as a novel species. A number of physiological and biochemical tests were able to distinguish strain WA201 from its closest phylogenetic neighbours. Therefore, it is proposed that isolate WA201 (=DSM 44830=LMG 22229) be considered the type strain representing a novel species, sp. nov.

Loading

Article metrics loading...

/content/journal/ijsem/10.1099/ijs.0.63361-0
2005-03-01
2020-12-01
Loading full text...

Full text loading...

/deliver/fulltext/ijsem/55/2/ijs550877.html?itemId=/content/journal/ijsem/10.1099/ijs.0.63361-0&mimeType=html&fmt=ahah

References

  1. Cashion P., Holder-Franklin M. A., McCully J., Franklin M. 1977; A rapid method for the base ratio determination of bacterial DNA. Anal Biochem 81:461–466 [CrossRef]
    [Google Scholar]
  2. De Ley J., Cattoir H., Reynaerts A. 1970; The quantitative measurement of DNA hybridization from renaturation rates. Eur J Biochem 12:133–142 [CrossRef]
    [Google Scholar]
  3. De Soete G. 1983; A least squares algorithm for fitting additive trees to proximity data. Psychometrika 48:621–663 [CrossRef]
    [Google Scholar]
  4. Doetsch R. N. 1981; Determinative methods of light microscopy. In Manual of Methods for General Bacteriology pp  21–33 Edited by Gerdhardt P., Murray R. G. E., Costilow R. N., Nester E. W., Wood W. A., Krieg N. R., Phillips G. B. Washington, DC: American Society for Microbiology;
    [Google Scholar]
  5. Fitch W. M. 1972; Toward defining the course of evolution: minimum change for a specific tree topology. Syst Zool 20:406–416
    [Google Scholar]
  6. Hasegawa T., Takizawa M., Tanida S. 1983; A rapid analysis for chemical grouping of aerobic actinomycetes. J Gen Appl Microbiol 29:319–322 [CrossRef]
    [Google Scholar]
  7. Hirsch P., Mevs U., Kroppenstedt R. M., Schumann P., Stackebrandt E. 2004; Cryptoendolithic actinomycetes from Antarctic sandstone rock samples: Micromonospora endolithica sp. nov. and two isolates related to Micromonospora coerulea Jensen 1932. Syst Appl Microbiol 27:166–174 [CrossRef]
    [Google Scholar]
  8. Huß V. A. R., Festl H., Schleifer K. H. 1983; Studies on the spectrophotometric determination of DNA hybridisation from renaturation rates. Syst Appl Microbiol 4:184–192 [CrossRef]
    [Google Scholar]
  9. Jahnke K.-D. 1992; basic computer program for evaluation of spectroscopic DNA renaturation data from GILFORD SYSTEM 2600 spectrophotometer on a PC/XT/AT type personal computer. J Microbiol Methods 15:61–73 [CrossRef]
    [Google Scholar]
  10. Kasai H., Tamura T., Harayama S. 2000; Intrageneric relationships among Micromonospora species deduced from gyrB -based phylogeny and DNA relatedness. Int J Syst Evol Microbiol 50:127–134 [CrossRef]
    [Google Scholar]
  11. Kawamoto I. 1989; Genus Micromonospora Ørskov 1923, 147AL . In Bergey's Manual of Systematic Bacteriology vol 4 pp  2442–2450 Edited by Williams S. T., Sharpe M. E., Holt J. G. Baltimore: Williams & Wilkins;
    [Google Scholar]
  12. Koch C., Kroppenstedt R. M., Stackebrandt E. 1996; Intrageneric relationships of the actinomycete genus Micromonospora . Int J Syst Bacteriol 46:383–387 [CrossRef]
    [Google Scholar]
  13. Kumar S., Tamura K., Jakobsen I. B., Nei M. 2001; mega2: molecular evolutionary genetics analysis software. Bioinformatics 17:1244–1245 [CrossRef]
    [Google Scholar]
  14. Lechevalier M. P., De Bièvre C., Lechevalier H. 1977; Chemotaxonomy of aerobic actinomycetes: phospholipid composition. Biochem Syst Ecol 5:249–260 [CrossRef]
    [Google Scholar]
  15. Lee S. D., Goodfellow M., Hah Y. C. 1999; A phylogenetic analysis of the genus Catellatospora based on 16S ribosomal DNA sequences, including transfer of Catellatospora matsumotoense to the genus Micromonospora as Micromonospora matsumotoense comb. nov. FEMS Microbiol Lett 178:349–354 [CrossRef]
    [Google Scholar]
  16. Lozano J. C., Blanco Rodríguez P., Tome F. V. 2002; Distribution of long-lived radionuclides of the 238U series in the sediments of a small river in a uranium mineralized region of Spain. J Environ Radioact 63:153–171 [CrossRef]
    [Google Scholar]
  17. Lüdemann G. M., Brodsky B. C. 1963; Taxonomy of gentamicin-producing Micromonospora . Antimicrob Agents Chemother 161:116–124
    [Google Scholar]
  18. Mandel M., Marmur J. 1968; Use of ultraviolet absorbance temperature profile for determining the guanine plus cytosine content of DNA. Methods Enzymol 12B:195–206
    [Google Scholar]
  19. Minnikin D. E., O'Donnell A. G., Goodfellow M., Alderson G., Athalye M., Schaal K., Parlett J. H. 1984; An integrated procedure for extracting bacterial isoprenoid quinones and polar lipids. J Microbiol Methods 2:233–241 [CrossRef]
    [Google Scholar]
  20. Ørskov J. 1923 Investigations into the Morphology of the Ray Fungi Copenhagen: Levin & Munksgaard;
    [Google Scholar]
  21. Rhuland L. E., Work E., Denman R. F., Hoare D. S. 1955; The behavior of the isomers of α , ε -diaminopimelic acid on paper chromatograms. J Am Chem Soc 77:4844–4846 [CrossRef]
    [Google Scholar]
  22. Rivas R., Sánchez M., Trujillo M. E., Zurdo-Piñeiro J. L., Mateos P. F., Martínez-Molina E., Velázquez E. 2003; Xylanimonas cellulosilytica gen. nov., sp. nov., a xylanolytic bacterium isolated from a decayed tree ( Ulmus nigra ). Int J Syst Evol Microbiol 53:99–103 [CrossRef]
    [Google Scholar]
  23. Saitou N., Nei M. 1987; The neighbor-joining method: a new method for reconstructing phylogenetic trees. Mol Biol Evol 4:406–425
    [Google Scholar]
  24. Schleifer K. H., Kandler O. 1972; Peptidoglycan types of bacterial cell walls and their taxonomic implications. Bacteriol Rev 36:407–477
    [Google Scholar]
  25. Schröder K.-H., Naumann L., Kroppenstedt R. M., Reischl U. 1997; Mycobacterium hassiacum sp. nov., a new rapidly growing thermophilic mycobacterium. Int J Syst Bacteriol 47:86–91 [CrossRef]
    [Google Scholar]
  26. Staneck J. L., Roberts G. D. 1974; Simplified approach to the identification of aerobic actinomycetes by thin-layer chromatography. Appl Microbiol 28:226–231
    [Google Scholar]
  27. Trujillo M. E., Velázquez E., Kroppenstedt R. M., Schumann P., Rivas R., Mateos P. F., Martínez-Molina E. 2004; Mycobacterium psychrotolerans sp. nov., isolated from pond water near a uranium mine. Int J Syst Evol Microbiol 54:1459–1463 [CrossRef]
    [Google Scholar]
  28. Wayne L. G., Brenner D. J., Colwell R. R. 9 other authors 1987; International Committee on Systematic Bacteriology. Report of the ad hoc committee on reconciliation of approaches to bacterial systematics. Int J Syst Bacteriol 37:463–464 [CrossRef]
    [Google Scholar]
  29. Williams S. T., Goodfellow M., Alderson G., Wellington E. M. H., Sneath P. H. A., Sackin M. J. 1983; Numerical classification of Streptomyces and related genera. J Gen Microbiol 129:1743–1813
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/ijsem/10.1099/ijs.0.63361-0
Loading
/content/journal/ijsem/10.1099/ijs.0.63361-0
Loading

Data & Media loading...

Supplements

Supplementary material 1

PDF

Supplementary material 2

PDF

Most cited this month Most Cited RSS feed

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error