1887

Abstract

A novel actinomycete strain, designated YIM 002, was isolated from a desert soil sample in Gansu Province, north-west China. This actinomycete isolate formed well-differentiated aerial and substrate mycelia. In the early stages of growth, the substrate mycelia fragmented into short or elongated rods. Chemotaxonomically, it contained -2,6-diaminopimelic acid in the cell wall. The cell-wall sugars contained ribose and glucose. Phospholipids present were phosphatidylinositol mannosides, phosphatidylinositol and diphosphatidylglycerol. MK-9(H) was the predominant menaquinone. The major fatty acids were anteiso C (35·92 %), anteiso C (15·84 %), iso C (10·40 %), iso C (7·07 %) and C 8 (9·37 %). The G+C content of the DNA was 70 mol%. Phylogenetic analysis and signature nucleotide data based on 16S rRNA gene sequences showed that strain YIM 002 is distinct from all recognized genera of the family in the suborder . On the basis of the phenotypic and genotypic characteristics, it is proposed that isolate YIM 002 be classified as a novel species in a new genus, gen. nov., sp. nov. The type strain is YIM 002 (=DSM 44835=CCTCC AA 204001=KCTC 19044).

Loading

Article metrics loading...

/content/journal/ijsem/10.1099/ijs.0.63353-0
2005-03-01
2021-03-01
Loading full text...

Full text loading...

/deliver/fulltext/ijsem/55/2/ijs550881.html?itemId=/content/journal/ijsem/10.1099/ijs.0.63353-0&mimeType=html&fmt=ahah

References

  1. Collins M. D., Dorsch M., Stackebrandt E. 1989; Transfer of Pimelobacter tumescens to Terrabacter gen. nov. as Terrabacter tumescens comb. nov. and of Pimelobacter jensenii to N o cardioides as Nocardioides jensenii comb. nov. Int J Syst Bacteriol 39:1–6 [CrossRef]
    [Google Scholar]
  2. Cui X. L., Mao P. H., Tseng M., Li W. J., Zhang L. P., Xu L. H., Jiang C. L. 2001; Streptomonospora salina gen. nov., a new member of the family Nocardiopsaceae . Int J Syst Evol Microbiol 51:357–363
    [Google Scholar]
  3. Felsenstein J. 1981; Evolutionary trees from DNA sequences: a maximum likelihood approach. J Mol Evol 17:368–376 [CrossRef]
    [Google Scholar]
  4. Felsenstein J. 1985; Confidence limits on phylogenies: an approach using the bootstrap. Evolution 39:783–791 [CrossRef]
    [Google Scholar]
  5. Fitch W. M., Margoliash E. 1967; Construction of phylogenetic trees: a method based on mutation distances as estimated from cytochrome c sequences is of general applicability. Science 155:279–284 [CrossRef]
    [Google Scholar]
  6. Kämpfer P., Steiof M., Dott W. 1991; Microbiological characterization of a fuel oil contaminated site including numerical identification of heterotrophic water and soil bacteria. Microb Ecol 21:227–251 [CrossRef]
    [Google Scholar]
  7. Kelly K. L. 1964 Inter-Society Color Council – National Bureau of Standards Color-Name Charts Illustrated with Centroid Colors Washington, DC: US Government Printing Office;
    [Google Scholar]
  8. Kimura M. 1980; A simple method for estimating evolutionary rates of base substitutions through comparative studies of nucleotide sequences. J Mol Evol 16:111–120 [CrossRef]
    [Google Scholar]
  9. Kimura M. 1983 The Neutral Theory of Molecular Evolution Cambridge: Cambridge University Press;
    [Google Scholar]
  10. Kroppenstedt R. M. 1982; Separation of bacterial menaquinones by HPLC using reverse phase (RP 18) and a silver loaded ion exchanger as stationary phases. J Liq Chromatogr 5:2359–2387 [CrossRef]
    [Google Scholar]
  11. Lechevalier M. P., Lechevalier H. A. 1970; Chemical composition as a criterion in the classification of aerobic actinomycetes. Int J Syst Bacteriol 20:435–443 [CrossRef]
    [Google Scholar]
  12. Marmur J. 1961; A procedure for the isolation of deoxyribonucleic acid from microorganisms. J Mol Biol 3:208–218 [CrossRef]
    [Google Scholar]
  13. Marmur J., Doty P. 1962; Determination of the base composition of deoxyribonucleic acid from its thermal denaturation temperature. J Mol Biol 5:109–118 [CrossRef]
    [Google Scholar]
  14. Miller E. S., Woese C. R., Brenner S. 1991; Description of the erythromycin-producing bacterium Arthrobacter sp. strain NRRL B-3381 as Aeromicrobium erythreum gen. nov., sp nov.. Int J Syst Bacteriol 41:363–368 [CrossRef]
    [Google Scholar]
  15. Minnikin D. E. O'Donnell A. G., Goodfellow M., Alderson G., Athalye M., Schaal A., Parlett J. H. 1984; An integrated procedure for the extraction of isoprenoid quinines and polar lipids. J Microbiol Methods 2:233–241 [CrossRef]
    [Google Scholar]
  16. Nesterenko O. A., Kvasnikov E. I., Nogina T. M. 1985; Nocardioidaceae fam. nov., a new family of the order Actinomycetales Buchanan 1917. Mikrobiol Zh 47:3–12
    [Google Scholar]
  17. O'Donnell A. G., Goodfellow M., Minnikin D. E. 1982; Lipids in the classification of Nocardioide s: reclassification of Arthrobacter simplex (Jensen) Lochhead in the genus N o cardioides (Prauser) emend.O'Donnell et al. as Nocardioides simplex comb. nov. Arch Microbiol 133:323–329 [CrossRef]
    [Google Scholar]
  18. Park Y.-H., Yoon J.-H., Shin Y. K., Suzuki K., Kudo T., Seino A., Kim H.-J., Lee J.-S., Lee S. T. 1999; Classification of ‘ Nocardioides fulvus ’ IFO 14399 and Nocardioides sp. ATCC 39419 in Kribbella gen. nov., as Kribbella flavida sp. nov. and Kribbella sandramycini sp. nov. Int J Syst Bacteriol 49:743–752 [CrossRef]
    [Google Scholar]
  19. Prauser H. 1976; Nocardioides , a new genus of the order Actinomycetales . Int J Syst Bacteriol 26:58–65 [CrossRef]
    [Google Scholar]
  20. Saitou N., Nei M. 1987; The neighbor-joining method: a new method for reconstructing phylogenetic trees. Mol Biol Evol 4:406–425
    [Google Scholar]
  21. Sasser M. 1990; Identification of bacteria by gas chromatography of cellular fatty acids. USFCC Newsl 20:16
    [Google Scholar]
  22. Shirling E. B., Gottlieb D. 1966; Methods for characterization of Streptomyces species. Int J Syst Bacteriol 16:313–340 [CrossRef]
    [Google Scholar]
  23. Sohn K., Hong S. G., Bae K. S., Chun J. 2003; Transfer of Hongia koreensis Lee et al . 2000 to the genus Kribbella Park et al . 1999 as Kribbella koreensis comb. nov. Int J Syst Evol Microbiol 53:1005–1007 [CrossRef]
    [Google Scholar]
  24. Stackebrandt E., Rainey F. A., Ward-Rainey N. L. 1997; Proposal for a new hierarchic classification system, Actinobacteria classis nov. Int J Syst Bacteriol 47:479–491 [CrossRef]
    [Google Scholar]
  25. Stanek J. L., Roberts G. D. 1974; Simplified approach to identification of aerobic actinomycetes by thin-layer chromatography. Appl Microbiol 28:226–231
    [Google Scholar]
  26. Suzuki K., Komagata K. 1983; Pimelobacter gen. nov., a new genus of coryneform bacteria with ll-diaminopimelic acid in the cell wall. J Gen Appl Microbiol 29:59–71 [CrossRef]
    [Google Scholar]
  27. Thompson J. D., Gibson T. J., Plewniak F., Jeanmougin F., Higgins D. G. 1997; The clustal_x windows interface: flexible strategies for multiple sequence alignment aided by quality analysis tools. Nucleic Acids Res 25:4876–4882 [CrossRef]
    [Google Scholar]
  28. Urzì C., Salamone P., Schumann P., Stackebrandt E. 2000; Marmoricola aurantiacus gen. nov. sp. nov. a coccoid member of the family Nocardioidaceae isolated from a marble statue. Int J Syst Evol Microbiol 50529–536 [CrossRef]
    [Google Scholar]
  29. Wang Y. M., Zhang Z. S., Xu X. L., Ruan J. S., Wang Y. 2001; Actinopolymorpha singaporensis gen. nov., sp. nov. a novel actinomycete from the tropical rainforest of Singapore. Int J Syst Evol Microbiol 51:467–473
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/ijsem/10.1099/ijs.0.63353-0
Loading
/content/journal/ijsem/10.1099/ijs.0.63353-0
Loading

Data & Media loading...

Supplements

Supplementary material 1

PDF

Supplementary material 2

PDF

Most cited this month Most Cited RSS feed

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error