1887

Abstract

The taxonomic position of three novel sea-water isolates was determined. The strains studied were strictly aerobic, heterotrophic, pigmented, motile by gliding, Gram-negative and oxidase-, catalase-, -galactosidase- and alkaline phosphatase-positive. 16S rRNA gene sequence phylogenetic analysis indicated that the strains KMM 6020, KMM 6021 and KMM 6028 occupied a distinct lineage within the family . The major respiratory quinone was MK-6. The predominant fatty acids were i15 : 0, i15 : 1, i15 : 0 3-OH, i17 : 19 and i17 : 0 3-OH. On the basis of phenotypic, chemotaxonomic, genotypic and phylogenetic characteristics, the novel bacteria were assigned to the genus gen. nov., as gen. nov., sp. nov. The type strain is KMM 6020 (=KCTC 12285=LMG 22569). From the results of the 16S rRNA gene sequence analysis and phenotypic features, the species [] Lewin 1969 is proposed to be reclassified in the new genus as gen. nov., comb. nov., with type strain CIP 104806 (=ATCC 23177=NCIMB 1399=LMG 1343).

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2005-01-01
2024-04-23
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References

  1. Bachmann B. J. 1955; Studies on Cytophaga fermentans n. sp., a facultatively anaerobic lower myxobacterium. J Gen Microbiol 13:541–551 [CrossRef]
    [Google Scholar]
  2. Barbeyron T., L'Haridon S., Corre E., Kloareg B., Potin P. 2001; Zobellia galactanovorans gen. nov., sp. nov., a marine species of Flavobacteriaceae isolated from red alga, and classification of [ Cytophaga ] uliginosa (ZoBell and Upham 1944) Reichenbach 1989 as Zobellia uliginosa gen. nov., comb. nov. Int J Syst Evol Microbiol 51:985–997 [CrossRef]
    [Google Scholar]
  3. Bernardet J.-F., Grimont P. A. D. 1989; Deoxyribonucleic acid relatedness and phenotypic characterization of Flexibacter columnaris sp.nov., nom. rev., Flexibacter psychrophilus sp. nov., nom. rev.,and Flexibacter maritimus Wakabayashi, Hikida, and Masumura 1986. Int J Syst Bacteriol 39:346–354 [CrossRef]
    [Google Scholar]
  4. Bernardet J.-F., Segers P., Vancanneyt M., Berthe F., Kersters K., Vandamme P. 1996; Cutting a Gordian knot: emended classification and description of the genus Flavobacterium , emended description of the family Flavobacteriaceae , and proposal of Flavobacterium hydatis nom. nov. (basonym Cytophaga aquatilis Strohl and Tait 1978. Int J Syst Bacteriol 46:128–148 [CrossRef]
    [Google Scholar]
  5. Bowman J. P. 2000; Description of Cellulophaga algicola sp. nov., isolated from the surfaces of Antarctic algae, and reclassification of Cytophaga uliginosa (ZoBell and Upham 1944) Reichenbach 1989 as Cellulophaga uliginosa comb. nov. Int J Syst Evol Microbiol 50:1861–1868
    [Google Scholar]
  6. Bowman J. P., McCammon S. A., Lewis T., Skerratt J. H., Brown J. L., Nichols D. S., McMeekin T. A. 1998; Psychroflexus torquis gen. nov., sp. nov., a psychrophilic species from Antarctic sea ice, and reclassification of Flavobacterium gondwanense (Dobson et al . 1993) as Psychroflexus gondwanense sp. nov., comb. nov. Microbiology 144:1601–1609 [CrossRef]
    [Google Scholar]
  7. Christensen P. 1980; Description and taxonomic status of Cytophaga heparina (Payza and Korn) comb. nov. (basonym: Flavobacterium heparinum Payza and Korn 1956. Int J Syst Bacteriol 30:473–475 [CrossRef]
    [Google Scholar]
  8. De Ley J., Cattoir H., Reynaerts A. 1970; The quantitative measurement of DNA hybridization from renaturation rates. Eur J Biochem 12:133–142 [CrossRef]
    [Google Scholar]
  9. Dobson S. J., Colwell R. R., McMeekin T. A., Franzmann P. D. 1993; Direct sequencing of the polymerase chain reaction-amplified 16S rRNA gene of Flavobacterium gondwanense sp. nov. and Flavobacterium salegens sp. nov., two new species from a hypersaline Antarctic lake. Int J Syst Bacteriol 43:77–83 [CrossRef]
    [Google Scholar]
  10. Donachie S. P., Bowman J. P., Alam M. 2004; Psychroflexus tropicus sp. nov., an obligately halophilic Cytophaga–Flavobacterium–Bacteroides group bacterium from an Hawaiian hypersaline lake. Int J Syst Evol Microbiol 54:935–940 [CrossRef]
    [Google Scholar]
  11. Felsenstein J. 1993 phylip (Phylogeny Inference Package), version 3.5c. Distributed by the author Department of Genetics, University of Washington; Seattle, USA:
    [Google Scholar]
  12. Haak S. K., Breznak J. A. 1993; Cytophaga xylanolytica sp. nov., a xylan-degrading, anaerobic gliding bacterium. Arch Microbiol 159:6–15 [CrossRef]
    [Google Scholar]
  13. Johansen J. E., Nielsen P., Sjøholm C. 1999; Description of Cellulophaga baltica gen.nov., sp. nov. and Cellulophaga fucicola gen. nov., sp. nov. and reclassification of [ Cytophaga ] lytica to Cellulophaga lytica gen. nov., comb. nov. Int J Syst Bacteriol 49:1231–1240 [CrossRef]
    [Google Scholar]
  14. Kim S. B., Falconer C., Williams E., Goodfellow M. 1998; Streptomyces thermocarboxydovorans sp. nov. and Streptomyces thermocarboxydus sp. nov., two moderately thermophilic carboxydotrophic species isolated from soil. Int J Syst Bacteriol 48:59–68 [CrossRef]
    [Google Scholar]
  15. Kimura M. 1980; A simple method for estimating evolutionary rates of base substitutions through comparative studies of nucleotide sequences. J Mol Evol 16:111–120 [CrossRef]
    [Google Scholar]
  16. Lewin R. A. 1969; A classification of flexibacteria. J Gen Microbiol 58:189–206 [CrossRef]
    [Google Scholar]
  17. Marmur J. 1961; A procedure for the isolation of deoxyribonucleic acid from microorganisms. J Mol Biol 3:208–218 [CrossRef]
    [Google Scholar]
  18. Marmur J., Doty P. 1962; Determination of the base composition of deoxyribonucleic acid from its thermal denaturation temperature. J Mol Biol 5:109–118 [CrossRef]
    [Google Scholar]
  19. Nakagawa Y., Yamasato K. 1996; Emendation of the genus Cytophaga and transfer of Cytophaga salmonicolor to Marinilabilia gen. nov.: phylogenetic analysis of the Flavobacterium–Cytophaga complex. Int J Syst Bacteriol 46:599–603 [CrossRef]
    [Google Scholar]
  20. Nakagawa Y., Hamana K., Sakane T., Yamasato K. 1997; Reclassification of Cytophaga aprica (Lewin 1969) Reichenbach 1989 in Flammeovirga gen.nov. as Flammeovirga aprica comb. nov. and Cytophaga diffluens (ex Stanier 1940; emend. Lewin 1969) Reichenbach 1989 in Persicobacter diffluens comb. nov. Int J Syst Bacteriol 47:220–223 [CrossRef]
    [Google Scholar]
  21. Nedashkovskaya O. I., Suzuki M., Vysotskii M. V., Mikhailov V. V. 2003a; Reichenbachia agariperforans gen. nov., sp. nov., a novel marine bacterium in the phylum Cytophaga Flavobacterium Bacteroides . Int J Syst Evol Microbiol 53:81–85 [CrossRef]
    [Google Scholar]
  22. Nedashkovskaya O. I., Suzuki M., Vysotskii M. V., Mikhailov V. V. 2003b; Vitellibacter vladivostokensis gen. nov., sp. nov., a new member of the phylum Cytophaga Flavobacterium Bacteroides . Int J Syst Evol Microbiol 53:1281–1286 [CrossRef]
    [Google Scholar]
  23. Nedashkovskaya O. I., Kim S. B., Han S. K. 7 other authors 2003c; Mesonia algae gen. nov., sp. nov., a novel marine bacterium of the family Flavobacteriaceae isolated from the green alga Acrosiphonia sonderi (Kütz) Kornm. Int J Syst Evol Microbiol 53:1967–1971 [CrossRef]
    [Google Scholar]
  24. Nedashkovskaya O. I., Suzuki M., Vancanneyt M., Cleenwerck I., Zhukova N. V., Vysotskii M. V., Mikhailov V. V., Swings J. 2004; Salegentibacter holothuriorum sp. nov., isolated from edible holothurian Apostichopus japonicus . Int J Syst Evol Microbiol 54:1107–1110 [CrossRef]
    [Google Scholar]
  25. Oyaizu H., Komagata K., Amemura A., Harada T. 1982; A succinoglycan-decomposing bacterium, Cytophaga arvensicola sp. nov. J Gen Appl Microbiol 28:369–388 [CrossRef]
    [Google Scholar]
  26. Oyaizu H., Komagata K., Amemura A., Harada T. 1983; Cytophaga arvensicola sp. nov. In Validation of the Publication of New Names and New Combinations Previously Effectively Published Outside the IJSB List no 10 Int J Syst Bacteriol 33:438–440 [CrossRef]
    [Google Scholar]
  27. Reichenbach H. 1989; Order Cytophagales Leadbetter 1974, 99AL . In Bergey's Manual of Systematic Bacteriology vol 3 pp  2011–2073 Edited by Staley J. T., Bryant M. P., Pfennig N., Holt J. C. Baltimore: Williams & Wilkins;
    [Google Scholar]
  28. Saitou N., Nei M. 1987; The neighbor-joining method: a new method for reconstructing phylogenetic trees. Mol Biol Evol 4:406–425
    [Google Scholar]
  29. Stanier R. Y. 1947; Studies on nonfruiting myxobacteria I Cytophaga johnsonae , n. sp. a chitin-decomposing myxobacterium. J Bacteriol 53:297–315
    [Google Scholar]
  30. Steyn P. L., Segers P., Vancanneyt M., Sandra P., Kersters K., Joubert J. J. 1998; Classification of heparinolytic bacteria into a new genus, Pedobacter , comprising four species: Pedobacter heparinus comb.nov., Pedobacter piscium comb. nov., Pedobacter africanus sp. nov. and Pedobacter saltans sp. nov.Proposal of the family Sphingobacteriaceae fam. nov. Int J Syst Bacteriol 48:165–177 [CrossRef]
    [Google Scholar]
  31. Strohl W. R., Tait L. R. 1978; Cytophaga aquatilis sp. nov., a facultative anaerobe isolated from the gills of freshwater fish. Int J Syst Bacteriol 28:293–303 [CrossRef]
    [Google Scholar]
  32. Suzuki M., Nakagawa Y., Harayama S., Yamamoto S. 1999; Phylogenetic analysis of genus Marinilabilia and related bacteria based on the amino acid sequences of GyrB and emended description of Marinilabilia salmonicolor with Marinilabilia agarovorans as its subjective synonym. Int J Syst Bacteriol 49:1551–1557 [CrossRef]
    [Google Scholar]
  33. Suzuki M., Nakagawa Y., Harayama S., Yamamoto S. 2001; Phylogenetic analysis and taxonomic study of marine Cytophaga- like bacteria: proposal for Tenacibaculum gen. nov. with Tenacibaculum maritimum comb.nov., and Tenacibaculum ovolyticum comb. nov., and description of Tenacibaculum mesophilum sp.nov. and Tenacibaculum amylolyticum sp. nov.. Int J Syst Evol Microbiol 51:1639–1652 [CrossRef]
    [Google Scholar]
  34. Takeuchi M., Yokota A. 1992; Proposals of Sphingobacterium faecium sp. nov., Sphingobacterium piscium sp. nov., Sphingobacterium heparinum comb. nov., Sphingobacterium thalpophilum comb. nov., and two genospecies of the genus Sphingobacterium and synonymy of Flavobacterium yabuuchiae and Sphingobacterium spiritivorum . J Gen Appl Microbiol 38:465–482 [CrossRef]
    [Google Scholar]
  35. Takeuchi M., Yokota A. 1993; Sphingobacterium heparinum comb. nov. In Validation of the Publication of New Names and New Combinations Previously Effectively Published Outside the IJSB List no 47 Int J Syst Bacteriol 43:864–865 [CrossRef]
    [Google Scholar]
  36. Van der Meulen H. J., Harder W., Veldkamp H. 1974; Isolation and characterization of Cytophaga flevensis sp. nov., a new agarolytic flexibacterium. Antonie van Leeuwenhoek 40:329–346 [CrossRef]
    [Google Scholar]
  37. Veldkamp H. 1961; A study of two marine agar-decomposing, facultatively anaerobic myxobacteria. J Gen Microbiol 26:331–342 [CrossRef]
    [Google Scholar]
  38. Wayne L. G., Brenner D. J., Colwell R. R. 9 other authors 1987; Report of the ad hoc committee on reconciliation of approaches to bacterial systematics. Int J Syst Bacteriol 37:463–464 [CrossRef]
    [Google Scholar]
  39. Winogradsky S. 1929; Études sur la microbiologie du sol – sur la dégradation de la cellulose dans le sol. Ann Inst Pasteur 43:549–633 (in French
    [Google Scholar]
  40. ZoBell C. E., Upham H. C. 1944; A list of marine bacteria including descriptions of sixty new species. Bull Scripps Inst Oceanogr Univ Calif 5:239–292
    [Google Scholar]
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