1887

Abstract

Five strains of an unusual Gram-negative, catalase-positive, oxidase-positive, coccobacillus-shaped bacterium isolated from the lungs and heart of pigs with pneumonia and pericarditis were characterized by phenotypic and molecular genetic methods. On the basis of cellular morphology and biochemical criteria, the isolates were tentatively assigned to the family , although they did not appear to correspond to any recognized genus or species. Comparative 16S rRNA gene sequencing showed that the five unidentified strains were phylogenetically highly related to each other and represent a hitherto unknown subline within the family . On the basis of both phenotypic and phylogenetic evidence, it is proposed that the unknown isolates from pigs be classified as a novel genus and species within the family , for which the name gen. nov., sp. nov. is proposed. The type strain of is 1258/02 (=CCUG 47806=CECT 5685).

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2005-03-01
2024-12-04
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References

  1. Barrett S. J., Sneath P. H. A. 1994; A numerical phenotypic study of the genus Neisseria . Microbiology 140:2867–2891 [CrossRef]
    [Google Scholar]
  2. Bøvre K. 1984; Family VIII. Neisseriaceae Prévot 1933, 119AL . In Bergey's Manual of Systematic Bacteriology vol. 1 pp  288–290 Edited by Krieg N. R., Holt J. G. Baltimore: Williams & Wilkins;
    [Google Scholar]
  3. Dewhirst F. E., Paster B. J., Bright P. L. 1989; Chromobacterium , Eikenella , Kingella , Neisseria , Simonsiella , and Vitreoscilla species comprise a major branch of the beta group Proteobacteria by 16S ribosomal ribonucleic acid sequence comparison: transfer of Eikenella and Simonsiella to the family Neisseriaceae (emend.). Int J Syst Bacteriol 39:258–266 [CrossRef]
    [Google Scholar]
  4. Dewhirst F. E., Chen C.-K. C., Paster B. J., Zambon J. J. 1993; Phylogeny of species in the family Neisseriaceae isolated from human dental plaque and description of Kingella orale sp. nov. Int J Syst Bacteriol 43:490–499 [CrossRef]
    [Google Scholar]
  5. Dolter J., Wong J., Janda J. M. 1998; Association of Neisseria cinerea with ocular infections in paediatric patients. J Infect 36:49–52
    [Google Scholar]
  6. Elad D., Shlomovitz S., Bernstein M., Bassan J. 1990; Neisseria cuniculi in ruminants: epidemiological aspects. Epidemiol Infect 105:559–563 [CrossRef]
    [Google Scholar]
  7. Hedlund B. P., Staley J. T. 2002; Phylogeny of the genus Simonsiella and other members of the Neisseriaceae . Int J Syst Evol Microbiol 52:1377–1382 [CrossRef]
    [Google Scholar]
  8. Jackson F. L., Goodman Y. 1984; Genus Eikenella Jackson and Goodman 1972, 74AL . In Bergey's Manual of Systematic Bacteriology vol 1 pp  591–597 Edited by Krieg N. R., Holt J. G. Baltimore: Williams & Wilkins;
    [Google Scholar]
  9. Juni E., Heym G. A. 1986; Psychrobacter immobilis gen. nov., sp. nov.: genospecies composed of gram-negative, aerobic, oxidase-positive coccobacilli. Int J Syst Bacteriol 36:388–391 [CrossRef]
    [Google Scholar]
  10. Kämpfer P., Kroppenstedt R. M. 1996; Numerical analysis of fatty acid patterns of coryneform bacteria and related taxa. Can J Microbiol 42:989–1005 [CrossRef]
    [Google Scholar]
  11. Kuhn D. A. 1981; The genera Simonsiella and Alysiella . In The Prokaryotes vol 1 pp  390–399 Edited by Starr M. P., Stolp H., Trüper H. G., Balows A., Schlegel H. G. New York: Springer;
    [Google Scholar]
  12. Larkin J. M. 1989; Family Simonsiellaceae Steed 1962, 615AL . In Bergey's Manual of Systematic Bacteriology vol 3p– 2107 Edited by Staley J. T., Bryant M. P., Pfennig N., Holt J. G. Baltimore: Williams & Wilkins;
    [Google Scholar]
  13. Mesbah M., Premachandran U., Whitman W. B. 1989; Precise measurement of the G+C content of deoxyribonucleic acid by high-performance liquid chromatography. Int J Syst Bacteriol 39:159–167 [CrossRef]
    [Google Scholar]
  14. Morse S. A., Knapp J. S. 1991; The genus Neisseria . In The Prokaryotes vol 3 pp  2496–2529 Edited by Balows A., Trüper H. G., Dworkin M., Harder K., Schleifer K.-H. New York: Springer;
    [Google Scholar]
  15. Moss M. O., Bryant T. N. 1982; DNA : rRNA hybridization studies of Chromobacterium fluviatile . J Gen Microbiol 128:829–834
    [Google Scholar]
  16. Nishimura Y., Kinpara M., Iizuka H. 1989; Mesophilobacter marinus gen. nov., sp. nov.: an aerobic coccobacillus isolated from seawater. Int J Syst Bacteriol 39:378–381 [CrossRef]
    [Google Scholar]
  17. Page R. D. M. 1996; treeview: an application to display phylogenetic trees on personal computers. Comput Appl Biosci 12:357–358
    [Google Scholar]
  18. Patureau D., Godon J.-J., Dabert P., Bouchez T., Bernet N., Delgenes J. P., Moletta R. 1998; Microvirgula aerodenitrificans gen. nov., sp. nov. a new Gram-negative bacterium exhibiting co-respiration of oxygen and nitrogen oxides up to oxygen-saturated conditions. Int J Syst Bacteriol 48:775–782 [CrossRef]
    [Google Scholar]
  19. Pearson W. R. 1994; Using the fasta program to search protein and DNA sequence databases. Methods Mol Biol 24:307–331
    [Google Scholar]
  20. Rasmussen S. W. 1995 DNATools, a software package for DNA sequence analysis Copenhagen: Carlsberg Laboratory;
    [Google Scholar]
  21. Rossau R., Van Landschoot W., Mannheim W., De Ley J. 1986; Inter- and intrageneric similarities of ribosomal ribonucleic acid cistrons of the Neisseriaceae . Int J Syst Bacteriol 36:323–332 [CrossRef]
    [Google Scholar]
  22. Rossau R., Vandenbussche G., Thielemans S., Segers P., Grosch H., Göthe E., Mannheim W., De Ley J. 1989; Ribosomal ribonucleic acid cistron similarities and deoxyribonucleic acid homologies of Neisseria , Kingella , Eikenella , Simonsiella , Alysiella , and Centers for Disease Control groups EF-4 and M-5 in the emended family Neisseriaceae . Int J Syst Bacteriol 39:185–198 [CrossRef]
    [Google Scholar]
  23. Saitou N., Nei M. 1987; The neighbor-joining method: a new method for reconstructing phylogenetic trees. Mol Biol Evol 4:406–425
    [Google Scholar]
  24. Snell J. J. S. 1984; Genus IV. Kingella Henriksen and Bøvre 1976, 449AL . In Bergey's Manual of Systematic Bacteriology vol 1 pp  307–309 Edited by Krieg N. R., Holt J. G. Baltimore: Williams & Wilkins;
    [Google Scholar]
  25. Vedros N. A. 1984; Genus I. Neisseria Trevisan 1885, 105AL . In Bergey's Manual of Systematic Bacteriology vol. 1 pp  290–296 Edited by Krieg N. R., Holt J. G. Baltimore: Williams & Wilkins;
    [Google Scholar]
  26. Wong J. D., Janda J. M. 1992; Association of an important Neisseria species, Neisseria elongata subsp. nitroreducens , with bacteremia, endocarditis, and osteomyelitis. J Clin Microbiol 30:719–720
    [Google Scholar]
  27. Yeruham I., Perl S., Elad D. 2001; Infectious bovine keratoconjunctivitis and lymphofollicular hyperplasia of the third eyelid in heifers. J Vet Med B Infect Dis Vet Public Health 48:137–141 [CrossRef]
    [Google Scholar]
  28. Yuen K. Y., Woo P. C., Teng J. L., Leung K. W., Wong M. K., Lau S. K. 2001; Laribacter hongkongensis gen. nov., sp. nov., a novel gram-negative bacterium isolated from a cirrhotic patient with bacteremia and empyema. J Clin Microbiol 39:4227–4232 [CrossRef]
    [Google Scholar]
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