1887

Abstract

A group of slowly growing photochromogenic mycobacteria was isolated from Chesapeake Bay striped bass () during an epizootic of mycobacteriosis. Growth characteristics, acid-fastness and 16S rRNA gene sequencing results were consistent with those of the genus . Biochemical reactions, growth characteristics and mycolic acid profiles (HPLC) resembled those of , a non-pigmented mycobacterium also isolated during the same epizootic. Sequencing of the 16S rRNA genes, the gene encoding the exported repeated protein () and the gene encoding the 65 kDa heat-shock protein () and restriction enzyme analysis of the gene demonstrated that this group of isolates is unique. Insertion sequences associated with , IS and IS, were detected by PCR. These isolates could be differentiated from other slowly growing pigmented mycobacteria by their inability to grow at 37 °C, production of niacin and urease, absence of nitrate reductase, negative Tween 80 hydrolysis and resistance to isoniazid (1 μg ml), -nitrobenzoic acid, thiacetazone and thiophene-2-carboxylic hydrazide. On the basis of this polyphasic study, it is proposed that these isolates represent a novel species, sp. nov. The type strain, L15, has been deposited in the American Type Culture Collection as ATCC BAA-883 and the National Collection of Type Cultures (UK) as NCTC 13318.

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2005-05-01
2021-02-27
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