1887

Abstract

Two strains of an unidentified, Gram-positive, catalase-negative, chain-forming, coccus-shaped organism recovered from seals were characterized using phenotypic and molecular taxonomic methods. Based on morphological and biochemical criteria the strains were tentatively identified as streptococci but they did not appear to correspond to any recognized species of the genus . Comparative 16S rRNA gene sequencing studies showed that the strains were closely related to each other and confirmed their placement in the genus . Sequence divergence values of >5 % with reference streptococcal species demonstrated the organisms from seals represent a novel species. SDS-PAGE analysis of whole-cell proteins confirmed that the two organisms were closely related to each other but were different from all currently defined streptococcal species. Based on biochemical criteria, molecular chemical and molecular genetic evidence, it is proposed that the unknown isolates from seals be assigned to a novel species of the genus , sp. nov. The type strain is M54/01/1 (=CCUG 48494=CIP 108309).

Loading

Article metrics loading...

/content/journal/ijsem/10.1099/ijs.0.63342-0
2005-01-01
2019-12-15
Loading full text...

Full text loading...

/deliver/fulltext/ijsem/55/1/ijs550271.html?itemId=/content/journal/ijsem/10.1099/ijs.0.63342-0&mimeType=html&fmt=ahah

References

  1. Collins, M. D., Hutson, R. A., Hoyles, L., Falsen, E., Nikolaitchouk, N. & Foster, G. ( 2001; ). Streptococcus ovis sp. nov., isolated from sheep. Int J Syst Evol Microbiol 51, 1147–1150.[CrossRef]
    [Google Scholar]
  2. Devriese, L. A., Pot, B., Vandamme, P., Kersters, K., Collins, M. D., Alvarez, N., Haesebrouck, F. & Hommez, J. ( 1997; ). Streptococcus hyovaginalis sp. nov. and Streptococcus thoraltensis sp. nov., from the genital tract of sows. Int J Syst Bacteriol 47, 1073–1077.[CrossRef]
    [Google Scholar]
  3. Facklam, R. ( 2002; ). What happened to the streptococci: overview of taxonomic and nomenclature changes. Clin Microbiol Rev 15, 613–630.[CrossRef]
    [Google Scholar]
  4. Lawson, P. A., Foster, G., Falsen, E., Davison, N. & Collins, M. D. ( 2004; ). Streptococcus halichoeri sp. nov., isolated from grey seals (Halichoerus grypus). Int J Syst Evol Microbiol 54, 1753–1756.[CrossRef]
    [Google Scholar]
  5. Mesbah, M., Premachandran, U. & Whitman, W. B. ( 1989; ). Precise measurement of the G+C content of deoxyribonucleic acid by high-performance liquid chromatography. Int J Syst Bacteriol 39, 159–167.[CrossRef]
    [Google Scholar]
  6. Nicholas, K. B., Nicholas, H. B., Jr & Deerfield, D. W., II ( 1997; ). GeneDoc: analysis and visualization of genetic variation. EMBNEW News 4, 14.
    [Google Scholar]
  7. Page, R. D. M. ( 1996; ). TreeView: an application to display phylogenetic trees on personal computer. Comput Appl Biosci 12, 357–358.
    [Google Scholar]
  8. Pier, G. B. & Madin, S. H. ( 1976; ). Streptococcus iniae sp. nov., a beta-hemolytic streptococcus from an Amazon freshwater dolphin, Inia geoffrensis. Int J Syst Bacteriol 26, 545–553.[CrossRef]
    [Google Scholar]
  9. Pot, B., Vandamme, P. & Kersters, K. ( 1994; ). Analysis of electrophoretic whole-organism protein fingerprints. In Modern Microbial Methods. Chemical Methods in Prokaryotic Systematics, pp. 493–521. Edited by M. Goodfellow & A. G. O'Donnell. Chichester: Wiley.
  10. Rasmussen, S. W. ( 1995; ). DNATools, a software package for DNA sequence analysis. Copenhagen: Carlsberg Laboratory.
  11. Saitou, N. & Nei, M. ( 1987; ). The neighbor-joining method: a new method for reconstructing phylogenetic trees. Mol Biol Evol 4, 406–425.
    [Google Scholar]
  12. Skaar, L., Gaustad, P., Tonjum, T., Holm, B. & Stenwig, H. ( 1994; ). Streptococcus phocae sp. nov., a new species isolated from clinical specimens of seals. Int J Syst Bacteriol 44, 646–650.[CrossRef]
    [Google Scholar]
  13. Vandamme, P., Pot, B., Gillis, M., DeVos, P., Kersters, K. & Swings, J. ( 1996; ). Polyphasic taxonomy, a consensus approach to bacterial systematics. Microbiol Rev 60, 407–438.
    [Google Scholar]
  14. Vandamme, P., Torck, U., Falsen, E., Pot, B., Goossens, H. & Kersters, K. ( 1998; ). Whole-cell protein electrophoretic analysis of viridans streptococci: evidence for heterogeneity among Streptococcus mitis biovars. Int J Syst Bacteriol 48, 117–125.[CrossRef]
    [Google Scholar]
  15. Vela, A. I., Fernández, E., Lawson, P. A., Latre, M. V., Falsen, E., Domínguez, L., Collins, M. D. & Fernández-Garayzábal, J. F. ( 2002; ). Streptococcus entericus sp. nov., isolated from cattle intestine. Int J Syst Evol Microbiol 52, 665–669.
    [Google Scholar]
  16. Weinstein, M. R., Litt, M., Kertesz, D. A. & 9 other authors ( 1997; ). Invasive infections due to a fish pathogen, Streptococcus iniae. N Engl J Med 337, 589–594.[CrossRef]
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/ijsem/10.1099/ijs.0.63342-0
Loading
/content/journal/ijsem/10.1099/ijs.0.63342-0
Loading

Data & Media loading...

Most Cited This Month

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error