1887

Abstract

The taxonomic position of a novel marine bacterium isolated from the green alga collected in the Sea of Japan was established. Cells of the strain studied, designated KMM 6017, were strictly aerobic, heterotrophic, pink-pigmented, non-motile by gliding, Gram-negative and oxidase-, catalase-, -galactosidase- and alkaline phosphatase-positive. 16S rRNA gene sequence analysis indicated that the strain occupied a distinct lineage within the phylum ‘’ and formed a cluster with [] and . The G+C content of the DNA of KMM 6017 was 40·2 mol%. The major respiratory quinone was MK-7. The predominant fatty acids were i15 : 1, i15 : 0 and i17 : 0 3-OH (34·2, 24 and 7·7 %, respectively). On the basis of phenotypic, chemotaxonomic, genotypic and phylogenetic characteristics, the novel bacterium was assigned to the genus gen. nov., as gen. nov., sp. nov. The type strain is KMM 6017 (=KCTC 12282=LMG 22567).

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2005-01-01
2019-10-16
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References

  1. Bernardet, J.-F., Segers, P., Vancanneyt, M., Berthe, F., Kersters, K. & Vandamme, P. ( 1996; ). Cutting a Gordian knot: emended classification and description of the genus Flavobacterium, emended description of the family Flavobacteriaceae, and proposal of Flavobacterium hydatis nom. nov. (basonym Cytophaga aquatilis Strohl and Tait 1978). Int J Syst Bacteriol 46, 128–148.[CrossRef]
    [Google Scholar]
  2. Bernardet, J.-F., Nakagawa, Y. & Holmes, B. ( 2002; ). Proposed minimal standards for describing new taxa of the family Flavobacteriaceae and emended description of the family. Int J Syst Evol Microbiol 52, 1049–1070.[CrossRef]
    [Google Scholar]
  3. Bowman, J. P. ( 2000; ). Description of Cellulophaga algicola sp. nov., isolated from the surfaces of Antarctic algae, and reclassification of Cytophaga uliginosa (ZoBell and Upham 1944) Reichenbach 1989 as Cellulophaga uliginosa comb. nov. Int J Syst Evol Microbiol 50, 1861–1868.
    [Google Scholar]
  4. Bowman, J. P., Nichols, C. M. & Gibson, J. A. E. ( 2003; ). Algoriphagus ratkowskyi gen. nov., sp. nov., Brumimicrobium glaciale gen. nov., sp. nov., Cryomorpha ignava gen. nov., sp. nov. and Crocinitomix catalasitica gen. nov., sp. nov., novel flavobacteria isolated from various polar habitats. Int J Syst Evol Microbiol 53, 1343–1355.[CrossRef]
    [Google Scholar]
  5. Brettar, I., Christen, R. & Höfle, M. G. ( 2004; ). Belliella baltica gen. nov., sp. nov., a novel marine bacterium of the CytophagaFlavobacteriumBacteroides group isolated from surface water of the central Baltic Sea. Int J Syst Evol Microbiol 54, 65–70.[CrossRef]
    [Google Scholar]
  6. Felsenstein, J. ( 1993; ). phylip (Phylogeny Inference Package), version 3.5c. Distributed by the author. Department of Genetics, University of Washington, Seattle, USA.
  7. Gerhardt, R., Murray, R. G. E., Wood, W. A. & Krieg, N. R. (editors) ( 1994; ). Methods for General and Molecular Bacteriology. Washington, DC: American Society for Microbiology.
  8. Kim, S. B., Falconer, C., Williams, E. & Goodfellow, M. ( 1998; ). Streptomyces thermocarboxydovorans sp. nov. and Streptomyces thermocarboxydus sp. nov., two moderately thermophilic carboxydotrophic species isolated from soil. Int J Syst Bacteriol 48, 59–68.[CrossRef]
    [Google Scholar]
  9. Kimura, M. ( 1980; ). A simple method for estimating evolutionary rates of base substitutions through comparative studies of nucleotide sequences. J Mol Evol 16, 111–120.[CrossRef]
    [Google Scholar]
  10. Lemos, M. L., Toranzo, A. E. & Barja, J. L. ( 1985; ). Modified medium for oxidation–fermentation test in the identification of marine bacteria. Appl Environ Microbiol 40, 1541–1543.
    [Google Scholar]
  11. Lewin, R. A. ( 1969; ). A classification of flexibacteria. J Gen Microbiol 58, 189–206.[CrossRef]
    [Google Scholar]
  12. Lewin, R. A. & Lounsbery, D. M. ( 1969; ). Isolation, cultivation, and characterization of flexibacteria. J Gen Microbiol 58, 145–170.[CrossRef]
    [Google Scholar]
  13. Marmur, J. ( 1961; ). A procedure for the isolation of deoxyribonucleic acid from microorganisms. J Mol Biol 3, 208–218.[CrossRef]
    [Google Scholar]
  14. Marmur, J. & Doty, P. ( 1962; ). Determination of the base composition of deoxyribonucleic acid from its thermal denaturation temperature. J Mol Biol 5, 109–118.[CrossRef]
    [Google Scholar]
  15. Nakagawa, Y. & Yamasato, K. ( 1996; ). Emendation of the genus Cytophaga and transfer of Cytophaga salmonicolor to Marinilabilia gen. nov.: phylogenetic analysis of the Flavobacterium–Cytophaga complex. Int J Syst Bacteriol 46, 599–603.[CrossRef]
    [Google Scholar]
  16. Nakagawa, Y., Hamana, K., Sakane, T. & Yamasato, K. ( 1997; ). Reclassification of Cytophaga aprica ( Lewin 1969 ) Reichenbach 1989 in Flammeovirga gen. nov. as Flammeovirga aprica comb. nov. and Cytophaga diffluens (ex Stanier 1940; emend. Lewin 1969 ) Reichenbach 1989 in Persicobacter diffluens comb. nov. Int J Syst Bacteriol 47, 220–223.[CrossRef]
    [Google Scholar]
  17. Nedashkovskaya, O. I., Suzuki, M., Vysotskii, M. V. & Mikhailov, V. V. ( 2003a; ). Reichenbachia agariperforans gen. nov., sp. nov., a novel marine bacterium in the phylum CytophagaFlavobacteriumBacteroides. Int J Syst Evol Microbiol 53, 81–85.[CrossRef]
    [Google Scholar]
  18. Nedashkovskaya, O. I., Kim, S. B., Han, S. K. & 7 other authors ( 2003b; ). Mesonia algae gen. nov., sp. nov., a novel marine bacterium of the family Flavobacteriaceae isolated from the green alga Acrosiphonia sonderi (Kütz) Kornm. Int J Syst Evol Microbiol 53, 1967–1971.[CrossRef]
    [Google Scholar]
  19. Nedashkovskaya, O. I., Vancanneyt, M., Van Trappen, S. & 7 other authors ( 2004; ). Description of Algoriphagus aquimarinus sp. nov., Algoriphagus chordae sp. nov. and Algoriphagus winogradskyi sp. nov., from sea water and algae, transfer of Hongiella halophila Yi and Chun 2004 to the genus Algoriphagus as Algoriphagus halophilus comb. nov. and emended descriptions of the genera Algoriphagus Bowman et al. 2003 and Hongiella Yi and Chun 2004 . Int J Syst Evol Microbiol 54, 1757–1764.[CrossRef]
    [Google Scholar]
  20. Raj, H. D. & Maloy, S. R. ( 1990; ). Proposal of Cyclobacterium marinus gen. nov., comb. nov., for a marine bacterium previously assigned to the genus Flectobacillus. Int J Syst Bacteriol 40, 337–347.[CrossRef]
    [Google Scholar]
  21. Reichenbach, H. ( 1989; ). The order Cytophagales Leadbetter 1974, 99AL. In Bergey's Manual of Systematic Bacteriology, vol. 3, pp. 2011–2073. Edited by J. T. Staley, M. P. Bryant, N. Pfennig & J. C. Holt. Baltimore: Williams & Wilkins.
  22. Saitou, N. & Nei, M. ( 1987; ). The neighbor-joining method: a new method for reconstructing phylogenetic trees. Mol Biol Evol 4, 406–425.
    [Google Scholar]
  23. Sly, L. I., Taghavi, M. & Fegan, M. ( 1998; ). Phylogenetic heterogeneity within the genus Herpetosiphon: transfer of the marine species Herpetosiphon cohaerens, Herpetosiphon nigricans and Herpetosiphon persicus to the genus Lewinella gen. nov. in the Flexibacter–Bacteroides–Cytophaga phylum. Int J Syst Bacteriol 48, 731–737.[CrossRef]
    [Google Scholar]
  24. Suzuki, M., Nakagawa, Y., Harayama, S. & Yamamoto, S. ( 1999; ). Phylogenetic analysis of genus Marinilabilia and related bacteria based on the amino acid sequences of GyrB and emended description of Marinilabilia salmonicolor with Marinilabilia agarovorans as its subjective synonym. Int J Syst Bacteriol 49, 1551–1557.[CrossRef]
    [Google Scholar]
  25. Van Trappen, S., Vandecandelaere, I., Mergaert, J. & Swings, J. ( 2004; ). Algoriphagus antarcticus sp. nov., a novel psychrophile from microbial mats in Antarctic lakes. Int J Syst Evol Microbiol 54, 1969–1973.[CrossRef]
    [Google Scholar]
  26. Veldkamp, H. ( 1961; ). A study of two marine agar-decomposing, facultatively anaerobic myxobacteria. J Gen Microbiol 26, 331–342.[CrossRef]
    [Google Scholar]
  27. Yi, H. & Chun, J. ( 2004; ). Hongiella mannitolivorans gen. nov., sp. nov., Hongiella halophila sp. nov. and Hongiella ornithinivorans sp. nov., isolated from tidal flat sediment. Int J Syst Evol Microbiol 54, 157–162.[CrossRef]
    [Google Scholar]
  28. Yoon, J.-H., Yeo, S.-H. & Oh, T.-K. ( 2004; ). Hongiella marincola sp. nov., isolated from sea water of the East Sea in Korea. Int J Syst Evol Microbiol 54, 1845–1848.[CrossRef]
    [Google Scholar]
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