1887

Abstract

A Gram-negative, motile, rod-shaped, psychrophilic and weakly halophilic bacterial strain, SL13, was isolated from deep-sea sediments (1450 m depth) of Edison Seamount in the western Pacific Ocean. Optimal growth of SL13 requires the presence of 1·5 % (w/v) NaCl, a pH of 6·0 and a temperature of 14 °C. The whole-cell fatty acid profile of the isolate includes C16 : 1 and C16 : 0 as major fatty acids and contains C20 : 53. This is consistent with corresponding data for . The DNA G+C content of strain SL13 is 43·8 mol%. Phylogenetic analyses of 16S rRNA gene sequences place this bacterium in the ‘’, within the genus . Sequence similarity analysis indicates that the closest relatives of strain SL13 are (99·3 %), (98·5 %) and (98·2 %). The DNA–DNA hybridization levels between the isolate and its closest known phylogenetic relatives, , and , are 27·1, 52·4 and 20·2 %, respectively. Thus strain SL13 represents a novel species of the genus , for which the name sp. nov. is proposed. The type strain is SL13 (=KCTC 12384=JCM 12947).

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2005-07-01
2020-09-25
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References

  1. Bae S. S., Lee J.-H., Kim S.-J. 2005; Bacillus alveayuensis sp. nov., a thermophilic bacterium isolated from deep-sea sediments of Ayu Trough. Int J Syst Evol Microbiol 55:1211–1215 [CrossRef]
    [Google Scholar]
  2. Baumann P., Baumann L. 1984; Genus II. Photobacterium Beijerinck 1889, 401AL . In Bergey's Manual of Systematic Bacteriology vol. 1 pp  539–545 Edited by Krieg N. R., Holt J. G. Baltimore: Williams & Wilkins;
    [Google Scholar]
  3. De Ley J., Cattoir H., Reynaerts A. 1970; The quantitative measurement of DNA hybridization from renaturation rates. Eur J Biochem 12:133–142 [CrossRef]
    [Google Scholar]
  4. DeLong E. F., Franks D. G., Yayanos A. A. 1997; Evolutionary relationships of cultivated psychrophilic and barophilic deep-sea bacteria. Appl Environ Microbiol 63:2105–2108
    [Google Scholar]
  5. Donovan T. J., Furniss A. L. 1984; Vibrios, aeromonas, plesiomonas and photobacterium. In Microbiological Methods pp  271–279 Edited by Collins C. H., Lyne P. M. London: Butterworth;
    [Google Scholar]
  6. Felsenstein J. 1985; Confidence limits on phylogenies: an approach using the bootstrap. Evolution 39:783–791 [CrossRef]
    [Google Scholar]
  7. Felsenstein J. 1993 phylip – Phylogeny Inference Package, version 3.5c. Distributed by the author. Department of Genome Sciences University of Washington; Seattle, USA:
    [Google Scholar]
  8. Fitch W. M. 1972; Towards defining the course of evolution: minimum change for a specific tree topology. Syst Zool 20:406–416
    [Google Scholar]
  9. Fitch W. M., Margoliash E. 1967; Construction of phylogenetic trees. Science 155:279–284 [CrossRef]
    [Google Scholar]
  10. Folch J., Lees M., Sloane Stanley G. H. 1957; A simple method for the isolation and purification of total lipids from animal tissues. J Biol Chem 226:497–509
    [Google Scholar]
  11. Jukes T. H., Cantor C. R. 1969; Evolution of protein molecules. In Mammalian Protein Metabolism vol 3 pp  21–132 Edited by Munro H. N. New York: Academic Press;
    [Google Scholar]
  12. Nogi Y., Masui N., Kato C. 1998; Photobacterium profundum sp. nov., a new, moderately barophilic bacterial species isolated from a deep-sea sediment. Extremophiles 2:1–7 [CrossRef]
    [Google Scholar]
  13. Okuzumi M., Hiraishi A., Kobayashi T., Fuhii T. 1994; Photobacterium histaminum sp. nov., a histamine-producing marine bacterium. Int J Syst Bacteriol 44:631–636 [CrossRef]
    [Google Scholar]
  14. Onarheim A. M., Wiik R., Burghardt J., Stackebrandt E. 1994; Characterization and identification of two Vibrio species indigenous to the intestine of fish in cold sea water; description of Vibrio iliopiscarius sp. nov. Syst Appl Microbiol 17:370–379 [CrossRef]
    [Google Scholar]
  15. Saitou N., Nei M. 1987; The neighbor-joining method: a new method for reconstructing phylogenetic trees. Mol Biol Evol 4:406–425
    [Google Scholar]
  16. Shieh W. Y., Chen Y.-W., Chaw S.-M., Chiu H.-H. 2003; Vibrio ruber sp. nov., a red, facultatively anaerobic, marine bacterium isolated from sea water. Int J Syst Evol Microbiol 53:479–484 [CrossRef]
    [Google Scholar]
  17. Sohn J. H., Lee J. H., Yi H., Chun J., Bae K. S., Ahn T.-Y., Kim S.-J. 2004a; Kordia algicida gen. nov., sp. nov., an algicidal bacterium isolated from red tide. Int J Syst Evol Microbiol 54:675–680 [CrossRef]
    [Google Scholar]
  18. Sohn J. H., Kwon K. K., Kang J. H., Jung H.-B., Kim S.-J. 2004b; Novosphingobium pentaromativorans sp. nov., a high-molecular-mass polycyclic aromatic hydrocarbon-degrading bacterium isolated from estuarine sediment. Int J Syst Evol Microbiol 54:1483–1487 [CrossRef]
    [Google Scholar]
  19. Stransky K., Jursik T., Vitek A., Skorepa J. 1992; An improved method of characterizing fatty acids by equivalent chain length values. J High Resolut Chromatogr 15:730–740 [CrossRef]
    [Google Scholar]
  20. Urakawa H., Kita-Tsukamoto K., Ohwada K. 1998; A new approach to separate the genus Photobacterium from Vibrio with RFLP patterns by Hha I digestion of PCR-amplified 16S rDNA. Curr Microbiol 36:171–174 [CrossRef]
    [Google Scholar]
  21. Urakawa H., Kita-Tsukamoto K., Ohwada K. 1999; Reassessment of the taxonomic position of Vibrio iliopiscarius (Onarheim et al . 1994) and proposal for Photobacterium iliopiscarium comb. nov. Int J Syst Bacteriol 49:257–260 [CrossRef]
    [Google Scholar]
  22. Wayne L. G., Brenner D. J., Colwell R. R. 9 other authors 1987; International Committee on Systematic Bacteriology. Report of the ad hoc committee on reconciliation of approaches of bacterial systematics. Int J Syst Bacteriol 37:463–464 [CrossRef]
    [Google Scholar]
  23. Weisburg W. G., Barns S. M., Pelletier D. A., Lane D. J. 1991; 16S ribosomal DNA amplification for phylogenetic study. J Bacteriol 173:697–703
    [Google Scholar]
  24. Xie C.-H., Yokota A. 2004; Transfer of Hyphomicrobium indicum to the genus Photobacterium as Photobacterium indicum comb. nov. Int J Syst Evol Microbiol 54:2113–2116 [CrossRef]
    [Google Scholar]
  25. Yoon J.-H., Lee J.-K., Kim Y.-O., Oh T. K. 2005; Photobacterium lipolyticum sp. nov., a bacterium with lipolytic activity isolated from the Yellow Sea in Korea. Int J Syst Evol Microbiol 55:335–339 [CrossRef]
    [Google Scholar]
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