1887

Abstract

A Gram-variable, motile, endospore-forming, slightly halophilic bacterial strain, designated SW-211, was isolated from a tidal flat of the Yellow Sea in Korea, and was characterized taxonomically by using a polyphasic approach. The organism grew optimally at 37 °C and in the presence of 2–3 % NaCl. Comparative 16S rRNA gene sequence analysis showed that strain SW-211 forms a distinct phylogenetic lineage within the radiation of the cluster comprising species. Strain SW-211 had cell-wall peptidoglycan based on -diaminopimelic acid. The predominant menaquinone was MK-7 and the major fatty acids were anteiso-C (34·8 %), iso-C (15·6 %), iso-C (12·5 %) and iso-C (10·0 %). The DNA G+C content was 35·2 mol%. Strain SW-211 exhibited levels of 16S rRNA gene sequence similarity of <96·2 % with respect to the type strains of recognized species. On the basis of its phenotypic properties and phylogenetic distinctiveness, strain SW-211 (=KCTC 3898=DSM 16303) was classified in the genus as the type strain of a novel species, for which the name sp. nov. is proposed.

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2005-09-01
2024-04-18
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References

  1. Baumann L., Baumann P. 1981; The marine Gram-negative eubacteria; genera Photobacterium , Beneckea , Alteromonas , Pseudomonas , and Alcaligenes . In The Prokaryotes . A Handbook on Habitats, Isolation, and Identification of Bacteria pp  1302–1330 Edited by Starr M. P., Stolp H., Trüper H. G., Balows A., Schlegel H. G. Berlin: Springer;
    [Google Scholar]
  2. Bruns A., Rohde M., Berthe-Corti L. 2001; Muricauda ruestringensis gen. nov. sp. nov. a facultatively anaerobic, appendaged bacterium from German North Sea intertidal sediment. Int J Syst Evol Microbiol 511997–2006 [CrossRef]
    [Google Scholar]
  3. Claus D., Berkeley R. C. W. 1986; Genus Bacillus Cohn 1872. In Bergey's Manual of Systematic Bacteriology vol 2 pp  1105–1140 Edited by Sneath P. H. A., Mair N. S., Sharpe M. E., Holt J. G. Baltimore: Williams & Wilkins;
    [Google Scholar]
  4. Cohen-Bazire G., Sistrom W. R., Stanier R. Y. 1957; Kinetic studies of pigment synthesis by nonsulfur purple bacteria. J Cell Comp Physiol 49:25–68 [CrossRef]
    [Google Scholar]
  5. Cowan S. T., Steel K. J. 1965 Manual for the Identification of Medical Bacteria London: Cambridge University Press;
    [Google Scholar]
  6. De Clerck E., Rodríguez-Díaz M., Vanhoutte T., Heyman J., Logan N. A., De Vos P. 2004; Anoxybacillus contaminans sp. nov. and Bacillus gelatini sp. nov., isolated from contaminated gelatin batches. Int J Syst Evol Microbiol 54:941–946 [CrossRef]
    [Google Scholar]
  7. Komagata K., Suzuki K. 1987; Lipids and cell-wall analysis in bacterial systematics. Methods Microbiol 19:161–207
    [Google Scholar]
  8. Lanyi B. 1987; Classical and rapid identification methods for medically important bacteria. Methods Microbiol 19:1–67
    [Google Scholar]
  9. Leifson E. 1963; Determination of carbohydrate metabolism of marine bacteria. J Bacteriol 85:1183–1184
    [Google Scholar]
  10. Li Z., Kawamura Y., Shida O., Yamagata S., Deguchi T., Ezaki T. 2002; Bacillus okuhidensis sp. nov., isolated from the Okuhida spa area of Japan. Int J Syst Evol Microbiol 52:1205–1209 [CrossRef]
    [Google Scholar]
  11. Logan N. A., Lebbe L., Verhelst A., Goris J., Forsyth G., Rodriguez-Diaz M., Heyndrickx M., De Vos P. 2002; Bacillus luciferensis sp. nov., from volcanic soil on Candlemas Island, South Sandwich archipelago. Int J Syst Evol Microbiol 52, 1985–1989 [CrossRef]
  12. Nielsen P., Fritze D., Priest F. G. 1995; Phenetic diversity of alkaliphilic Bacillus strains: proposal for nine new species. Microbiology 141:1745–1761 [CrossRef]
    [Google Scholar]
  13. Priest F. G., Goodfellow M., Todd C. 1988; A numerical classification of the genus Bacillus . J Gen Microbiol 134:1847–1882
    [Google Scholar]
  14. Reva O. N., Smirnov V. V., Pettersson B., Priest F. G. 2002; Bacillus endophyticus sp. nov., isolated from inner tissues of cotton plants ( Gossypium sp.). Int J Syst Evol Microbiol 52:101–107
    [Google Scholar]
  15. Sasser M. 1990 Identification of Bacteria by Gas Chromatography of Cellular Fatty Acids Newark, DE: MIDI Inc;
    [Google Scholar]
  16. Spanka R., Fritze D. 1993; Bacillus cohnii sp. nov., a new, obligately alkaliphilic, oval-spore-forming Bacillus species with ornithine and aspartic acid instead of diaminopimelic acid in the cell wall. Int J Syst Bacteriol 43:150–156 [CrossRef]
    [Google Scholar]
  17. Stackebrandt E., Goebel B. M. 1994; Taxonomic note: a place for DNA-DNA reassociation and 16S rRNA sequence analysis in the present species definition in bacteriology. Int J Syst Bacteriol 44:846–849 [CrossRef]
    [Google Scholar]
  18. Staley J. T. 1968; Prosthecomicrobium and Ancalomicrobium : new prosthecate freshwater bacteria. J Bacteriol 95:1921–1942
    [Google Scholar]
  19. Suresh K., Prabagaran S. R., Sengupta S., Shivaji S. 2004; Bacillus indicus sp. nov., an arsenic-resistant bacterium isolated from an aquifer in West Bengal, India. Int J Syst Evol Microbiol 54:1369–1375 [CrossRef]
    [Google Scholar]
  20. Tamaoka J., Komagata K. 1984; Determination of DNA base composition by reverse-phase high-performance liquid chromatography. FEMS Microbiol Lett 25:125–128 [CrossRef]
    [Google Scholar]
  21. Täubel M., Kämpfer P., Buczolits S., Lubitz W., Busse H.-J. 2003; Bacillus barbaricus sp. nov., isolated from an experimental wall painting. Int J Syst Evol Microbiol 53:725–730 [CrossRef]
    [Google Scholar]
  22. Yi H., Chang Y.-H., Oh H. W., Bae K. S., Chun J. 2003; Zooshikella ganghwensis gen. nov., sp. nov. isolated from tidal flat sediments. Int J Syst Evol Microbiol 53:1013–1018 [CrossRef]
    [Google Scholar]
  23. Yoon J.-H., Kim H., Kim S.-B., Kim H.-J., Kim W. Y., Lee S. T., Goodfellow M., Park Y.-H. 1996; Identification of Saccharomonospora strains by the use of genomic DNA fragments and rRNA gene probes. Int J Syst Bacteriol 46:502–505 [CrossRef]
    [Google Scholar]
  24. Yoon J.-H., Lee S. T., Park Y.-H. 1998; Inter- and intraspecific phylogenetic analysis of the genus Nocardioides and related taxa based on 16S rRNA gene sequences. Int J Syst Bacteriol 48:187–194 [CrossRef]
    [Google Scholar]
  25. Yoon J.-H., Kim I.-G., Kang K. H., Oh T.-K., Park Y.-H. 2003a; Bacillus marisflavi sp. nov. and Bacillus aquimaris sp. nov., isolated from sea water of a tidal flat of the Yellow Sea in Korea. Int J Syst Evol Microbiol 53:1297–1303 [CrossRef]
    [Google Scholar]
  26. Yoon J.-H., Weiss N., Kang K. H., Oh T.-K., Park Y.-H. 2003b; Planococcus maritimus sp. nov., isolated from sea water of a tidal flat in Korea. Int J Syst Evol Microbiol 53:2013–2017 [CrossRef]
    [Google Scholar]
  27. Yoon J.-H., Kang K. H., Oh T.-K., Park Y.-H. 2004a; Shewanella gaetbuli sp. nov., a slight halophile isolated from a tidal flat in Korea. Int J Syst Evol Microbiol 54:487–491 [CrossRef]
    [Google Scholar]
  28. Yoon J.-H., Kim I.-G., Oh T.-K., Park Y.-H. 2004b; Microbulbifer maritimus sp. nov., isolated from an intertidal sediment from the Yellow Sea, Korea. Int J Syst Evol Microbiol 54:1111–1116 [CrossRef]
    [Google Scholar]
  29. Yoon J.-H., Lee C.-H., Oh T.-K. 2005; Bacillus cibi sp. nov., isolated from jeotgal, a traditional Korean fermented seafood. Int J Syst Evol Microbiol 55:733–736 [CrossRef]
    [Google Scholar]
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