1887

Abstract

The taxonomic position of a rhizosphere isolate, strain 52108a, was determined using a polyphasic approach. The strain was found to have chemical and morphological properties consistent with its assignment to the genus . An almost complete 16S rRNA gene sequence determined for the strain was aligned with corresponding sequences of representatives of the genus and related taxa using three tree-making algorithms. The organism formed a distinct phyletic line within the clade and was most closely related to (98·9 %), (99·0 %), (98·4 %) and (98·2 %), but was readily distinguished from representatives of these species using a combination of phenotypic properties. The combined genotypic and phenotypic data show that the strain should be classified in the genus as a novel species. The name proposed is sp. nov., with the type strain 52108a (=AS 4.1878=DSM 44826).

Loading

Article metrics loading...

/content/journal/ijsem/10.1099/ijs.0.63329-0
2005-03-01
2021-02-27
Loading full text...

Full text loading...

/deliver/fulltext/ijsem/55/2/ijs550707.html?itemId=/content/journal/ijsem/10.1099/ijs.0.63329-0&mimeType=html&fmt=ahah

References

  1. Chun J. 1995; Computer-assisted classification and identification of actinomycetes . PhD thesis University of; Newcastle, UK:
  2. Chung Y. R., Sung K. C., Mo H. K., Son D. Y., Nam J. S., Chun J., Bae K. S. 1999; Kitasatospora cheerisanensis sp. nov., a new species of the genus Kitasatospora that produces an antifungal agent. Int J Syst Bacteriol 49:753–758 [CrossRef]
    [Google Scholar]
  3. Collins M. D. 1985; Isoprenoid quinone analyses in bacterial classification and identification. In Chemical Methods in Bacterial Systematics pp  267–287 Edited by Goodfellow M., Minnikin D. E. London: Academic Press;
    [Google Scholar]
  4. Felsenstein J. 1993 phylip (Phylogeny Inference Package), version 3.5c. Distributed by the author Department of Genetics, University of Washington; Seattle, USA:
    [Google Scholar]
  5. Groth I., Schütze B., Boettcher T., Pullen C. B., Rodriguez C., Leistner E., Goodfellow M. 2003; Kitasatospora putterlickiae sp. nov., isolated from rhizosphere soil, transfer of Streptomyces kifunensis to the genus Kitasatospora as Kitasatospora kifunensis comb. nov., and emended description of Streptomyces aureofaciens Duggar 1948. Int J Syst Evol Microbiol 53:2033–2040 [CrossRef]
    [Google Scholar]
  6. Groth I., Rodríguez C., Schütze B., Schmitz P., Leistner E., Goodfellow M. 2004; Five novel Kitasatospora species from soil: Kitasatospora arboriphila sp.nov., K. gansuensis sp. nov., K. nipponensis sp. nov., K. paranensis sp.nov. and K. terrestris sp. nov. Int J Syst Evol Microbiol 54:2121–2129 [CrossRef]
    [Google Scholar]
  7. Hasegawa T., Takizawa M., Tanida S. 1983; A rapid analysis for chemical grouping of aerobic actinomycetes. J Gen Appl Microbiol 29:319–322 [CrossRef]
    [Google Scholar]
  8. Hopwood D. A., Bibb M. J., Chater K. F. 7 other authors 1985; Genetic Manipulation of Streptomyces. A Laboratory Manual Norwich: The John Innes Foundation;
    [Google Scholar]
  9. Huang Y., Cui Q., Wang L., Rodriguez C., Quintana E., Goodfellow M., Liu Z. 2004; Streptacidiphilus jiangxiensis sp. nov., a novel actinomycete isolated from acidic rhizosphere soil in China. Antonie van Leeuwenhoek 86:159–165 [CrossRef]
    [Google Scholar]
  10. Jones K. L. 1949; Fresh isolates of actinomycetes in which the presence of sporogenous aerial mycelia is a fluctuating characteristic. J Bacteriol 57:141–145
    [Google Scholar]
  11. Kämpfer P., Kroppenstedt R. M. 1996; Numerical analysis of fatty acid patterns of coryneform bacteria and related taxa. Can J Microbiol 42:989–1005 [CrossRef]
    [Google Scholar]
  12. Kawato M., Shinobu R. 1959; On Streptomyces herbaricolor nov. sp. Supplement: a simple technique for the microscopic observation. Mem Osaka Univ Lib Arts Educ 8:114–119
    [Google Scholar]
  13. Kim S. B., Falconer C., Williams E., Goodfellow M. 1998; Streptomyces thermocarboxydovorans sp. nov. and Streptomyces thermocarboxydus sp. nov., two moderately thermophilic carboxydotrophic species from soil. Int J Syst Bacteriol 48:59–68 [CrossRef]
    [Google Scholar]
  14. Kusakabe H., Isono K. 1992; Kitasatosporia cystarginea sp. nov. In Validation of the Publication of New Names and New Combinations Previously Effectively Published Outside the IJSB , List no. 41. Int J Syst Bacteriol 42:327–328 [CrossRef]
    [Google Scholar]
  15. Küster E. 1959; Outline of a comparative study on criteria used in characterisation of the actinomycetes. Int Bull Bacteriol Nomencl Taxon 9:97–104
    [Google Scholar]
  16. Labeda D. P. 1988; Kitasatosporia mediocidica sp. nov. Int J Syst Bacteriol 38:287–290 [CrossRef]
    [Google Scholar]
  17. Minnikin D. E., O'Donnell A. G., Goodfellow M., Alderson G., Athalye M., Schaal A., Parlett J. H. 1984; An integrated procedure for the extraction of bacterial isoprenoid quinones and polar lipids. J Microbiol Methods 2:233–241 [CrossRef]
    [Google Scholar]
  18. Nakagaito Y., Yokota A., Hasegawa T. 1992; Three new species of the genus Streptomyces : Streptomyces cochleatus sp. nov., Streptomyces paracochleatus sp. nov., and Streptomyces azaticus sp. nov. J Gen Appl Microbiol 38:105–120 [CrossRef]
    [Google Scholar]
  19. Nakagaito Y., Shimazu A., Yokota A., Hasegawa T. 1993a; Streptomyces cochleatus sp. nov., Streptomyces paracochleatus sp. nov. and Streptomyces azaticus sp. nov.In Validation of the Publication of New Names and New Combinations Previously Effectively Published Outside the IJSB , List no. 44. Int J Syst Bacteriol 43:188–189 [CrossRef]
    [Google Scholar]
  20. Nakagaito Y., Shimazu A., Yokota A., Hasegawa T. 1993b; Streptomyces kifuensis sp. nov. In Validation of the Publication of New Names and New Combinations Previously Effectively Published Outside the IJSB , List no. 46. Int J Syst Bacteriol 43:624 [CrossRef]
    [Google Scholar]
  21. O'Donnell A. G., Falconer C., Goodfellow M., Ward A. C., Williams E. 1993; Biosystematics and diversity amongst novel carboxydotrophic actinomycetes. Antonie van Leeuwenhoek 64:325–340
    [Google Scholar]
  22. Ōmura S., Takahashi Y., Iwai Y., Tanaka H. 1982; Kitasatosporia , a new genus of the order Actinomycetales . J Antibiot 35:1013–1019 [CrossRef]
    [Google Scholar]
  23. Ōmura S., Takahashi Y., Iwai Y., Tanaka H. 1983; Kitasatosporia setalba sp. nov. In Validation of the Publication of New Names and New Combinations Previously Effectively Published Outside the IJSB , List no. 11. Int J Syst Bacteriol 33:672–674 [CrossRef]
    [Google Scholar]
  24. Ōmura S., Takahashi Y., Iwai Y., Tanaka H. 1985; Revised nomenclature of Kitasatosporia setalba . Int J Syst Bacteriol 35:221 [CrossRef]
    [Google Scholar]
  25. Reed J. F., Cummings R. W. 1945; Soil reaction-glass electrodes and colorimetric methods for determining pH value of soil. Soil Sci 59:97–104 [CrossRef]
    [Google Scholar]
  26. Sasser M. 1990; Identification of bacteria by gas chromatography of cellular fatty acids . Technical Note 101: Newark, DE: MIDI Inc;
    [Google Scholar]
  27. Staneck J. L., Roberts G. D. 1974; Simplified approach to identification of aerobic actinomycetes by thin layer chromatography. Appl Microbiol 28:225–231
    [Google Scholar]
  28. Tajima K., Takahashi Y., Seino A., Iwai Y., Ōmura S. 2001; Description of two novel species of the genus Kitasatospora Ōmura et al 1982 Kitasatospora cineracea sp. nov. and Kitasatospora niigatensis sp. nov.. Int J Syst Evol Microbiol 51:1765–1771 [CrossRef]
    [Google Scholar]
  29. Takahashi Y., Iwai Y., Ōmura S. 1985; Kitasatosporia griseola sp. nov. In Validation of the Publication of New Names and New Combinations Previously Effectively Published Outside the IJSB List no 19 Int J Syst Bacteriol 35:535 [CrossRef]
    [Google Scholar]
  30. Uchida K., Aida K. 1977; Acyl type of bacterial cell wall: its simple identification by colorimetric method. J Gen Appl Microbiol 23:249–260 [CrossRef]
    [Google Scholar]
  31. Van de Peer Y., De Wachter R. 1994; treecon for Windows: a software package for the construction and drawing of evolutionary trees for the Microsoft Windows environment. Comput Appl Biosci 10:569–570
    [Google Scholar]
  32. Wang Y., Zhang Z., Ruan J. 1996a; Phylogenetic analysis reveals new relationships among members of the genera Microtetraspora and Microbispora . Int J Syst Bacteriol 46:658–663 [CrossRef]
    [Google Scholar]
  33. Wang Y., Zhang Z., Ruan J. 1996b; A proposal to transfer Microbispora bispora (Lechevalier 1965) to a new genus, Thermobispora gen. nov., as Thermobispora bispora comb. nov. Int J Syst Bacteriol 46:933–938 [CrossRef]
    [Google Scholar]
  34. Wang L., Cui Q., Huang Y., Xie Q., Zhang Y., Liu Z. 2003; Isolation of acidophilic and acidoduric streptomycetes using a dispersion and differential centrifugation approach. Microbiology (English translation of Mikrobiologiya ). 30104–106
  35. Wayne L. G., Brenner D. J., Colwell R. R. 9 other authors 1987; Report of the ad hoc committee on reconciliation of approaches to bacterial systematics. Int J Syst Bacteriol 37:463–464 [CrossRef]
    [Google Scholar]
  36. Wellington E. M. H., Stackebrandt E., Sanders D., Wolstrup J., Jorgensen N. O. G. 1992; Taxonomic status of Kitasatosporia , and proposed unification with Streptomyces on the basis of phenotypic and 16S rRNA analysis and emendation of Streptomyces Waksman and Henrici 1943, 339AL . Int J Syst Bacteriol 42:156–160 [CrossRef]
    [Google Scholar]
  37. Williams S. T., Goodfellow M., Alderson G., Wellington E. M. H., Sneath P. H. A., Sackin M. J. 1983; Numerical classification of Streptomyces and related genera. J Gen Microbiol 129:1743–1813
    [Google Scholar]
  38. Wu C., Lu X., Qin M., Wang Y., Ruan J. 1989; Analysis of menaquinone compounds in microbial cells by HPLC. Microbiology (English translation of Mikrobiologiya) 16176–178
    [Google Scholar]
  39. Zhang Z., Wang Y., Ruan J. 1997; A proposal to revive the genus Kitasatospora (Omura, Takahashi, Iwai, and Tanaka 1982). Int J Syst Bacteriol 47:1048–1054 [CrossRef]
    [Google Scholar]
  40. Zhou Z.-H., Liu Z.-H., Qian Y.-D., Kim S. B., Goodfellow M. 1998; Saccharopolyspora spinosporotrichia sp. nov., a novel actinomycete from soil. Int J Syst Bacteriol 48:53–58 [CrossRef]
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/ijsem/10.1099/ijs.0.63329-0
Loading
/content/journal/ijsem/10.1099/ijs.0.63329-0
Loading

Data & Media loading...

Most cited this month Most Cited RSS feed

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error