1887

Abstract

A Gram-negative, polarly flagellated bacterium was isolated from a sea-water sample collected from the Karadag Natural Reserve of the Eastern Crimea and characterized to clarify its taxonomic position. 16S rRNA gene sequence-based phylogenetic analysis of this novel organism revealed , , , and ‘’ as its closest relatives (similarity 95–97 %). The G+C content of the DNA was 46·5 mol%. The organism grew between 4 and 33 °C, tolerated 10 % NaCl, was slightly alkaliphilic and was not able to degrade starch, gelatin, agar or Tween 80. Phosphatidylethanolamine (53·4 %) and phosphatidylglycerol (46·6 %) were the predominant phospholipids. The major fatty acids were 16 : 0 (15·5 %), 16 : 17 (26·7 %) and 18 : 17 (47·1 %). The phylogenetic, genetic and physiological properties of the organism placed it within a novel species, proposed as sp. nov., the type strain of which is 46-16 (=LMG 22531=KMM 3492=UCM 11075).

Loading

Article metrics loading...

/content/journal/ijsem/10.1099/ijs.0.63326-0
2005-01-01
2021-04-10
Loading full text...

Full text loading...

/deliver/fulltext/ijsem/55/1/ijs550275.html?itemId=/content/journal/ijsem/10.1099/ijs.0.63326-0&mimeType=html&fmt=ahah

References

  1. Barry A. I. 1980; Procedures and theoretical considerations for testing antimicrobial agents in agar media. In Antibiotics in Laboratory Medicine pp  10–16 Edited by Logan V. Baltimore: William & Wilkins;
    [Google Scholar]
  2. Baumann L., Baumann P., Mandel M., Allen R. D. 1972; Taxonomy of aerobic marine eubacteria. J Bacteriol 3:402–429
    [Google Scholar]
  3. Baumann P., Gauthier M. J., Baumann L. 1984; Genus Alteromonas Baumann, Baumann, Mandel and Allen 1972, 418AL . In Bergey's Manual of Systematic Bacteriology vol 1 pp  343–352 Edited by Krieg N. R., Holt J. G. Baltimore: Williams & Wilkins;
    [Google Scholar]
  4. Berry V., Gascuel O. 1996; Interpretation of bootstrap trees: threshold of clade selection and induced gain. Mol Biol Evol 13:999–1011 [CrossRef]
    [Google Scholar]
  5. Bligh E. G., Dyer W. J. 1959; A rapid method of lipid extraction and purification. Can J Biochem Physiol 7:911–915
    [Google Scholar]
  6. Christie W. W. 1982 Lipid Analysis: Isolation, Separation, Identification and Structural Analysis of Lipids Oxford: Pergamon Press;
    [Google Scholar]
  7. Felsenstein J. 1993 phylip: Phylogeny Inference Package, version 3.5c Department of Genetics, University of Washington; Seattle, WA, USA:
    [Google Scholar]
  8. Gascuel O. 1997; bionj: an improved version of the NJ algorithm based on a simple model of sequence data. Mol Biol Evol 14:685–695 [CrossRef]
    [Google Scholar]
  9. Gauthier M. J., Breittmayer V. A. 1992; The genera Alteromonas and Marinomonas . In The Prokaryotes. A Handbook on the Biology of Bacteria: Ecophysiology, Isolation, Identification Applications vol. 3 pp  3046–3070 Edited by Balows A., Trüper H. G., Dworkin M., Harber H., Schleifer K.-H. Berlin: Springer;
    [Google Scholar]
  10. Ivanova E. P., Kiprianova E. A., Mikhailov V. V., Levanova F. G., Garagulya A. G., Gorshkova N. M., Yumoto N., Yoshikawa S. 1996; Characterization and identification of marine Alteromonas nigrifaciens strains and emendation of the description. Int J Syst Bacteriol 46:223–228 [CrossRef]
    [Google Scholar]
  11. Ivanova E. P., Kiprianova E. A., Mikhailov V. V. 7 other authors 1998; Phenotypic diversity of Pseudoalteromonas citrea from different marine habitats and emendation of the description. Int J Syst Bacteriol 48:247–256 [CrossRef]
    [Google Scholar]
  12. Ivanova E. P., Zhukova N. V., Svetashev V. I., Gorshkova N. M., Kurilenko V. V., Frolova G. M., Mikhailov V. V. 2000; Evaluation of phospholipid and fatty acid compositions as chemotaxonomic markers of Alteromonas -like proteobacteria. Curr Microbiol 41:341–345 [CrossRef]
    [Google Scholar]
  13. Ivanova E. P., Sawabe T., Gorshkova N. M., Svetashev V. I., Mikhailov V. V., Nicolau D. V., Christen R. 2001; Shewanella japonica sp. nov. Int J Syst Evol Microbiol 51:1027–1033 [CrossRef]
    [Google Scholar]
  14. Ivanova E. P., Nedashkovskaya O. I., Zhukova N. V., Nicolau D. V., Christen R., Mikhailov V. V. 2003a; Shewanella waksmanii sp. nov., isolated from a sipuncula ( Phascolosoma japonicum . Int J Syst Evol Microbiol 53:1471–1477 [CrossRef]
    [Google Scholar]
  15. Ivanova E. P., Sawabe T., Zhukova N. V. 8 other authors 2003b; Occurrence and diversity of mesophilic Shewanella strains isolated from the North-West Pacific Ocean. Syst Appl Microbiol 26:293–301 [CrossRef]
    [Google Scholar]
  16. Ivanova E. P., Gorshkova N. M., Sawabe T. 8 other authors 2004; Sulfitobacter delicatus sp. nov. and Sulfitobacter dubius sp. nov., respectively, from a starfish ( Stellaster equestris ) and sea grass ( Zostera marina . Int J Syst Evol Microbiol 54:475–480 [CrossRef]
    [Google Scholar]
  17. Keswani J., Whitman W. B. 2001; Relationship of 16S rRNA sequence similarity to DNA hybridization in prokaryotes. Int J Syst Evol Microbiol 51:667–678
    [Google Scholar]
  18. Marmur J. 1961; A procedure for the isolation of deoxyribonucleic acid from microorganisms. J Mol Biol 3:208–218 [CrossRef]
    [Google Scholar]
  19. Marmur J., Doty P. 1962; Determination of the base composition of deoxyribonucleic acid from its thermal denaturation temperature. J Mol Biol 5:109–118 [CrossRef]
    [Google Scholar]
  20. Romanenko L. A., Uchino M., Mikhailov V. V., Zhukova N. V., Uchimura T. 2003; Marinomonas primoryensis sp. nov., a novel psychrophile isolated from coastal sea-ice in the Sea of Japan. Int J Syst Evol Microbiol 53:829–832 [CrossRef]
    [Google Scholar]
  21. Rosselló-Mora R., Amann R. 2001; The species concept for prokaryotes. FEMS Microbiol Rev 25:39–67 [CrossRef]
    [Google Scholar]
  22. Smibert R. M., Krieg N. R. 1994; Phenotypic characterization. In Methods for General and Molecular Bacteriology pp  607–654 Edited by Gerhardt P., Murray R. G. E., Wood W. A., Krieg N. R. Washington, DC: American Society for Microbiology;
    [Google Scholar]
  23. Solano F., Sanchez-Amat A. 1999; Studies on the phylogenetic relationships of melanogenic marine bacteria: proposal of Marinomonas mediterranea sp. nov. Int J Syst Bacteriol 49:1241–1246 [CrossRef]
    [Google Scholar]
  24. Solano F., Garcia E., Perez de Egea E., Sanchez-Amat A. 1997; Isolation and characterization of strain MMB-1, a novel melanogenic marine bacterium. Appl Environ Microbiol 63:3499–3506
    [Google Scholar]
  25. Stackebrandt E., Goebel B. M. 1994; Taxonomic note: a place for DNA-DNA reassociation and 16S rRNA sequence analysis in the present species definition in bacteriology. Int J Syst Bacteriol 44:846–849 [CrossRef]
    [Google Scholar]
  26. van Landschoot A., De Ley J. 1983; Intra- and intergeneric similarities of the rRNA cistrons of Alteromonas , Marinomonas (gen. nov.) and some other Gram-negative bacteria. J Gen Microbiol 129:3057–3074
    [Google Scholar]
  27. van Landschoot A., De Ley J. 1984; Marinomonas gen. nov., Marinomonas communis comb. nov. and Marinomonas vaga comb. nov. In Validation of the Publication of New Names and New Combinations Previously Effectively Published Outside the IJSB List no 13 Int J Syst Bacteriol 34:91–92 [CrossRef]
    [Google Scholar]
  28. Vaskovsky V. E., Terekhova T. A. 1979; HPTLC of phospholipid mixtures containing phosphatidylglycerol. J High Resolut Chromatogr Chromatogr Commun 2:671–672 [CrossRef]
    [Google Scholar]
  29. Vaskovsky V. E., Kostetsky E. Y., Vasendin I. M. 1975; A universal reagent for phospholipid analysis. J Chromatogr 114:129–141 [CrossRef]
    [Google Scholar]
  30. Wayne L. G., Brenner D. J., Colwell R. R. 9 other authors 1987; International Committee on Systematic Bacteriology. Report of the ad hoc committee on reconciliation of approaches to bacterial systematics. Int J Syst Bacteriol 37:463–464 [CrossRef]
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/ijsem/10.1099/ijs.0.63326-0
Loading
/content/journal/ijsem/10.1099/ijs.0.63326-0
Loading

Data & Media loading...

Most cited this month Most Cited RSS feed

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error