1887

Abstract

Members of the genus are ubiquitous in nature and can be isolated from almost any freshwater environment where dissolved oxygen exists. This genus is composed of a variety of pink-pigmented, facultatively methylotrophic (PPFM) bacteria. During a screening programme to monitor the bacterial population present in the drinking water of a municipal water supply in Seville (Spain) during the year 2003, five strains of PPFM bacteria were isolated and characterized. Analysis of their complete 16S rRNA gene sequences revealed that they constituted two separate phylogenetic groups (strains GP34 and GR18, and strains GR16, GP22 and GP32, respectively) showing highest similarity to members of the genus . The highest 16S rRNA sequence similarities of strain GP34 were found with respect to the type strains of (96·6 %) and (96·4 %) and the highest 16S rRNA sequence similarities of strain GR16 were to the type strains of (96·0 %) and (95·8 %). The G+C content of their DNA ranged from 66·5 to 67·8 mol%. DNA–DNA hybridization studies confirmed that they constituted two separate genospecies. On the basis of this phenotypic, phylogenetic and genotypic study, two novel species of the genus are proposed: sp. nov., with type strain GP34 (CECT 5997=CCM 7219=DSM 16372=CIP 108332), and sp. nov., with type strain GR16 (CECT 5998=CCM 7218=DSM 16371=CIP 108333).

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2005-01-01
2020-01-23
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