1887

Abstract

Twenty morphologically different strains were chosen from French wheat sourdough isolates. Cells were Gram-positive, non-spore-forming, non-motile rods. The isolates were identified using amplified-fragment length polymorphism, randomly amplified polymorphic DNA and 16S rRNA gene sequence analysis. All isolates were members of the genus . They were identified as representing , , , and . However, two isolates (LP38 and LP39) could be clearly discriminated from recognized species on the basis of genotyping methods. 16S rRNA gene sequence similarity and DNA–DNA relatedness data indicate that the two strains belong to a novel species, for which the name is proposed. The type strain is LP38 (=DSM 16381=CIP 108387=TMW 1.1236).

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2005-03-01
2019-10-19
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References

  1. Abo-Elnaga, I. G. & Kandler, O. ( 1965; ). Zur Taxonomie der Gattung Lactobacillus Beijerinck. I. Das Subgenus Streptobacterium Orla-Jensen. Zentbl Bakteriol Parasitenkd Infektionskr Hyg 119, 1–36 (in German).
    [Google Scholar]
  2. Bervas, E. ( 1991; ). Mise au point de levains bactériens pour la panification. Thèse de Doctorat, Université Blaise Pascal, Clermont-Ferrand, France (in French).
  3. Böcker, G., Vogel, R. F. & Hammes, W. P. ( 1990; ). Lactobacillus sanfrancisco als stabiles element in einem Reinzucht-Sauerteig-Präparat. Getreide Mehl Brot 44, 269–274 (in German).
    [Google Scholar]
  4. De Ley, J., Cattoir, H. & Reynaerts, A. ( 1970; ). The quantitative measurement of DNA hybridization from renaturation rates. Eur J Biochem 12, 133–142.[CrossRef]
    [Google Scholar]
  5. De Man, J. C., Rogosa, M. & Sharpe, M. E. ( 1960; ). A medium for the cultivation of lactobacilli. J Appl Bacteriol 23, 130–135.[CrossRef]
    [Google Scholar]
  6. Ehrmann, M. A., Müller, M. R. A. & Vogel, R. F. ( 2003; ). Molecular analysis of sourdough reveals Lactobacillus mindensis sp. nov. Int J Syst Evol Microbiol 53, 7–13.[CrossRef]
    [Google Scholar]
  7. Escara, J. F. & Hutton, J. R. ( 1980; ). Thermal stability and renaturation of DNA in dimethyl sulfoxide solutions: acceleration of the renaturation rate. Biopolymers 19, 1315–1327.[CrossRef]
    [Google Scholar]
  8. Gabriel, V., Lefebvre, D., Vayssier, Y. & Faucher, C. ( 1999; ). Characterisation of microflora from natural sourdoughs. Microbiol Alim Nutr 17, 171–179.
    [Google Scholar]
  9. Gobetti, M., Corsetti, A. & Rossi, J. ( 1994; ). The sourdough microflora: interactions between lactic acid bacteria and yeasts. Appl Microbiol Biotechnol 41, 456–460.[CrossRef]
    [Google Scholar]
  10. Hammes, W. P. & Gänzle, M. G. ( 1998; ). Sourdough breads and related products. In Microbiology of Fermented Foods, pp. 199–216. Edited by B. J. Wood. London: Chapman & Hall.
  11. Hammes, W. P. & Tichaczek, P. S. ( 1994; ). The potential of lactic acid bacteria for the production of safe and wholesome food. Z Lebensm Unters Forsch 198, 193–201.[CrossRef]
    [Google Scholar]
  12. Hammes, W. P. & Vogel, R. F. ( 1995; ). The genus Lactobacillus. In The Lactic Acid Bacteria, the Genera of Lactic Acid Bacteria, vol. 2, pp. 19–54. Edited by B. J. B. Wood & W. H. Holzapfel. London: Blackie Academic & Professional.
  13. Hammes, W. P., Stolz, P. & Gänzle, M. ( 1996; ). Metabolism of lactobacilli in traditional sourdoughs. Adv Food Sci 18, 176–184.
    [Google Scholar]
  14. Herrero, M., Mayo, B., Gonzalez, B. & Suarez, J. E. ( 1996; ). Evaluation of technologically important traits in lactic acid bacteria isolated from spontaneous fermentations. J Appl Bacteriol 81, 565–570.
    [Google Scholar]
  15. Huß, V. A. R., Festl, H. & Schleifer, K. H. ( 1983; ). Studies on the spectrophotometric determination of DNA hybridisation from renaturation rates. Syst Appl Microbiol 4, 184–192.[CrossRef]
    [Google Scholar]
  16. Infantes, M. ( 1992; ). Etude des levains naturels de panification provenant de différentes régions françaises. Thèse de Doctorat, Institut National Agronomique Paris – Grignon, France (in French).
  17. Kandler, O. & Weiss, N. ( 1986; ). Genus Lactobacillus Beijerinck 1901, 212AL. In Bergey's Manual of Systematic Bacteriology, vol. 2, pp. 1209–1234. Edited by P. H. A. Sneath, N. S. Mair, M. E. Sharpe & J. G. Holt. Baltimore: Williams & Wilkins.
  18. Kline, L. & Sugihara, T. F. ( 1971; ). Microorganisms of the San Francisco sour dough bread process. II. Isolation and characterization of undescribed bacterial species responsible for the souring activity. Appl Microbiol 21, 459–465.
    [Google Scholar]
  19. Marmur, J. ( 1961; ). A procedure for the isolation of deoxyribonucleic acid for micro-organisms. J Mol Biol 3, 208–218.[CrossRef]
    [Google Scholar]
  20. Meroth, C. B., Hammes, W. P. & Hertel, C. ( 2004; ). Characterisation of the microbiota of rice sourdoughs and description of Lactobacillus spicheri sp. nov. Syst Appl Microbiol 27, 151–159.[CrossRef]
    [Google Scholar]
  21. Mesbah, M., Premachandran, U. & Whitman, W. B. ( 1989; ). Precise measurement of the G+C content of deoxyribonucleic acid by high-performance liquid chromatography. Int J Syst Bacteriol 39, 159–167.[CrossRef]
    [Google Scholar]
  22. Müller, M. R. A., Wolfrum, G., Stolz, P., Ehrmann, M. A. & Vogel, R. F. ( 2001; ). Monitoring the growth of Lactobacillus species during a rye flour fermentation. Food Microbiol 18, 217–227.[CrossRef]
    [Google Scholar]
  23. Onno, B. & Roussel, P. ( 1994; ). Technologie et microbiologie de la panification au levain. In Bactéries Lactiques, vol. II, pp. 293–321. Edited by H. de Roissart & F. M. Luquet. Grenoble: Lorica (in French).
  24. Schleifer, K. H. & Kandler, O. ( 1972; ). Peptidoglycan types of bacterial cell walls and their taxonomic implications. Bacteriol Rev 36, 407–477.
    [Google Scholar]
  25. Schmidt, H., Ehrmann, M. A., Vogel, R. F., Taniwaki, M. H. & Niessen, L. ( 2003; ). Molecular typing of Aspergillus ochraceus and construction of species specific SCAR-primers based on AFLP. Syst Appl Microbiol 26, 138–146.[CrossRef]
    [Google Scholar]
  26. Stackebrandt, E. & Goebel, B. M. ( 1994; ). Taxonomic note: a place for DNA–DNA reassociation and 16S rRNA sequence analysis in the present species definition in bacteriology. Int J Syst Bacteriol 44, 846–849.[CrossRef]
    [Google Scholar]
  27. Stolz, P., Hammes, W. P. & Vogel, R. F. ( 1996; ). Maltose-phosphorylase and hexokinase activity in lactobacilli from traditionally prepared sourdoughs. Adv Food Sci 18, 1–6.
    [Google Scholar]
  28. Torriani, S., Clementi, F., Vancanneyt, M., Hoste, B., Dellaglio, F. & Kersters, K. ( 2001; ). Differentiation of Lactobacillus plantarum, L. pentosus and L. paraplantarum species by RAPD-PCR and AFLP. Syst Appl Microbiol 24, 554–560.[CrossRef]
    [Google Scholar]
  29. Vancanneyt, M., Neysens, P., De Wachter, M. & 8 other authors ( 2005; ). Lactobacillus acidifarinae sp. nov. and Lactobacillus zymae sp. nov., from wheat sourdoughs. Int J Syst Evol Microbiol 55, 615–620.[CrossRef]
    [Google Scholar]
  30. Vogel, R. F., Knorr, R., Müller, M. R. A., Steudel, U., Gänzle, M. G. & Ehrmann, M. A. ( 1999; ). Non-dairy lactic fermentations: the cereal world. Antonie van Leeuwenhoek 76, 403–411.[CrossRef]
    [Google Scholar]
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vol. , part 2, pp. 763 - 767

RAPD-PCR patterns of sourdough isolates and selected reference lactobacilli. Dendrogram derived from UPGMA cluster analysis (correlation coefficient expressed as percentages). Sourdough isolates are indicated by ‘a’ and sourdough isolates identified by partial 16S rRNA gene sequencing are indicated by ‘b’; all other strains are reference strains.

AFLP patterns of sourdough isolates and selected reference lactobacilli. Dendrogram derived from UPGMA cluster analysis (correlation coefficient expressed as percentages). Sourdough isolates are indicated as outlined in the legend to Fig. A.

[PDF file of Figs A and B](144 KB)



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