1887

Abstract

Three Gram-variable, rod-shaped bacterial strains, TF-16, TF-19 and TF-80, were isolated from a tidal flat of Daepo Beach (Yellow Sea) near Mokpo City, Korea, and their taxonomic positions were investigated by a polyphasic approach. These isolates grew optimally in the presence of 2 % NaCl and at 30 °C. Their peptidoglycan types were based on -Lys–Gly. The predominant menaquinone detected in the three strains was MK-7. The three strains contained large amounts of the branched fatty acids iso-C, anteiso-C, iso-C and iso-C. The DNA G+C contents of strains TF-16, TF-19 and TF-80 were 48·6, 48·4 and 48·0 mol%, respectively. The three strains formed a coherent cluster with species in a phylogenetic tree based on 16S rRNA gene sequences. They showed closest phylogenetic affiliation to , with 16S rRNA gene sequence similarity values of 98·1–98·3 %. The three strains exhibited 16S rRNA gene sequence similarity values of 94·0–94·6 % to the type strains of other species. Levels of DNA–DNA relatedness indicated that strains TF-16 and TF-19 and strain TF-80 are members of two species that are separate from . On the basis of phenotypic, phylogenetic and genetic data, strains TF-16 and TF-19 and strain TF-80 represent two novel species in the genus ; the names sp. nov. (type strain TF-16=KCTC 19035=DSM 16306; reference strain TF-19) and sp. nov. (type strain TF-80=KCTC 19036=DSM 16307) are proposed.

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2005-03-01
2020-09-23
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