1887

Abstract

A taxonomic study of two crude-oil-degrading, Gram-positive bacterial strains, designated BAS69 and BNP48, revealed that they represent two novel species. 16S rRNA gene sequence similarity to their closest phylogenetic neighbours was 98·5 % for BAS69 ( DSM 15019 and DSM 20169) and 99 % for BNP48 ( DSM 20143). Levels of DNA–DNA relatedness to the closest phylogenetic neighbours of both strains were between 11 and 38 %. According to phylogenetic analysis, the two strains are distinguishable from all recognized species of . Morphological and physiological characteristics of strains BAS69 and BNP48 were different from those of phylogenetically closely related species. The diamino acid in the cell-wall peptidoglycan of BAS69 is lysine and of BNP48 is ornithine. The major menaquinones are MK-11 and MK-12 for both strains. Based on their ability to degrade crude oil, the name sp. nov. is proposed for strain BAS69 (=DSM 16091=NCIMB 14003) and is proposed for strain BNP48 (=DSM 16089=NCIMB 14002).

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2005-03-01
2020-01-17
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