1887

Abstract

A taxonomic study of two crude-oil-degrading, Gram-positive bacterial strains, designated BAS69 and BNP48, revealed that they represent two novel species. 16S rRNA gene sequence similarity to their closest phylogenetic neighbours was 98·5 % for BAS69 ( DSM 15019 and DSM 20169) and 99 % for BNP48 ( DSM 20143). Levels of DNA–DNA relatedness to the closest phylogenetic neighbours of both strains were between 11 and 38 %. According to phylogenetic analysis, the two strains are distinguishable from all recognized species of . Morphological and physiological characteristics of strains BAS69 and BNP48 were different from those of phylogenetically closely related species. The diamino acid in the cell-wall peptidoglycan of BAS69 is lysine and of BNP48 is ornithine. The major menaquinones are MK-11 and MK-12 for both strains. Based on their ability to degrade crude oil, the name sp. nov. is proposed for strain BAS69 (=DSM 16091=NCIMB 14003) and is proposed for strain BNP48 (=DSM 16089=NCIMB 14002).

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2005-03-01
2020-09-30
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References

  1. Behrendt U., Ulrich A., Schumann P. 2001; Description of Microbacterium foliorum sp. nov. and Microbacterium phyllosphaerae sp. nov., isolated from the phyllosphere of grasses and the surface litter after mulching the sward, and reclassification of Aureobacterium resistens . (Funke et al . 1998) as Microbacterium resistens comb. nov. Int J Syst Evol Microbiol 51, 1267–1276
  2. Bock M., Kämpfer P., Bosecker K., Dott W. 1994; Isolation and characterization of heterotrophic, aerobic bacteria from oil storage caverns in northern Germany. Appl Microbiol Biotechnol 42:463–468 [CrossRef]
    [Google Scholar]
  3. Bosecker K., Teschner M., Wehner H. 1991; Biodegradation of crude oils. In Developments in Geochemistry 6: Diversity of Environmental Biogeochemistry pp  195–204 Edited by Berthelin J. Amsterdam: Elsevier;
    [Google Scholar]
  4. Burghardt F. 1992 Mikrobiologische Diagnostik Stuttgart: Thieme-Verlag;
    [Google Scholar]
  5. Cashion P., Holder-Franklin M. A., McCully J., Franklin M. 1977; A rapid method for the base ratio determination of bacterial DNA. Anal Biochem 81:461–466 [CrossRef]
    [Google Scholar]
  6. Collins M. D., Bradbury J. F. 1999; The Genera Agromyces Aureobacterium , Clavibacter , Curtobacterium , and Microbacterium . In The Prokaryotes release 3.0 http://141.150.157.117:8080/prokPUB/chaprender/jsp/showchap.jsp?chapnum=062&initsec=01_00
    [Google Scholar]
  7. Collins M. D., Pirouz T., Goodfellow M., Minnikin D. E. 1977; Distribution of menaquinones in actinomycetes and corynebacteria. J Gen Microbiol 100:221–230 [CrossRef]
    [Google Scholar]
  8. De Ley J., Cattoir H., Reynaerts A. 1970; The quantitative measurement of DNA hybridization from renaturation rates. Eur J Biochem 12:133–142 [CrossRef]
    [Google Scholar]
  9. De Soete G. 1983; A least squares algorithm for fitting additive trees to proximity data. Psychometrica 48:621–626 [CrossRef]
    [Google Scholar]
  10. Escara J. F., Hutton J. R. 1980; Thermal stability and renaturation of DNA in dimethyl sulfoxide solutions: acceleration of the renaturation rate. Biopolymers 19:1315–1327 [CrossRef]
    [Google Scholar]
  11. Fedorak P. M., Westlake D. W. S. 1981; Microbial degradation of aromatics and saturates in Prudhoe Bay crude oil as determined by glass capillary gas chromatography. Can J Microbiol 27:432–443 [CrossRef]
    [Google Scholar]
  12. Felsenstein J. 1993 phylip (Phylogeny Inference Package), version 3.5c. Distributed by the author Department of Genetics, University of Washington; Seattle, USA:
    [Google Scholar]
  13. Gerhardt P., Murray R. G. E., Wood W. A., Hodson R. E., Whitman W. B. 1994 Methods for General and Molecular Bacteriology Washington, DC: American Society for Microbiology;
    [Google Scholar]
  14. Groth I., Schumann P., Weiss N., Martin K., Rainey F. A. 1996; Agrococcus jenensis gen. nov., sp. nov. a new genus of actinomycetes with diaminobutyric acid in the cell wall. Int J Syst Bacteriol 46:234–239 [CrossRef]
    [Google Scholar]
  15. Huß V. A. R., Festl H., Schleifer K. H. 1983; Studies on the spectrophotometric determination of DNA hybridization from renaturation rates. Syst Appl Microbiol 4:184–192 [CrossRef]
    [Google Scholar]
  16. Jahnke K. D. 1992; Basic computer program for evaluation of spectroscopic DNA renaturation data from GILFORD System 2600 spectrometer on a PC/XT/AT type personal computer. J Microbiol Methods 15:61–73 [CrossRef]
    [Google Scholar]
  17. Jobson A., Cook F. D., Westlake D. W. S. 1972; Microbial utilization of crude oil. Appl Microbiol 23:1082–1089
    [Google Scholar]
  18. Jukes T. H., Cantor C. R. 1969; Evolution of protein molecules. In Mammalian Protein Metabolism pp  21–132 Edited by Munro H. N. New York: Academic Press;
    [Google Scholar]
  19. Kämpfer P., Steiof M., Dott W. 1991; Microbiological characterization of a fuel-oil contaminated site including numerical identification of heterotrophic water and soil bacteria. Microb Ecol 21:227–251 [CrossRef]
    [Google Scholar]
  20. Laffineur K., Avesani V., Cornu G., Charlier J., Janssens M., Wauters G., Delmée M. 2003; Bacteremia due to a novel Microbacterium species in a patient with leukemia and description of Microbacterium paraoxydans sp. nov. J Clin Microbiol 41:2242–2246 [CrossRef]
    [Google Scholar]
  21. Maidak B. L., Cole J. R., Parker C. T. Jr 10 other authors 1999; A new version of the RDP (Ribosomal Database Project). Nucleic Acids Res 27:171–173 [CrossRef]
    [Google Scholar]
  22. Rainey F. A., Ward-Rainey N., Kroppenstedt R. M., Stackebrandt E. 1996; The genus Nocardiopsis represents a phylogenetically coherent taxon and a distinct actinomycete lineage: proposal of Nocardiopsaceae fam. nov. Int J Syst Bacteriol 46:1088–1092 [CrossRef]
    [Google Scholar]
  23. Rosenberg E. 2000; Hydrocarbon-oxidizing bacteria. In The Prokaryotes , release 3.1 http://141.150.157.117:8080/prokPUB/chaprender/jsp/showchap.jsp?chapnum=247&initsec=04_03
    [Google Scholar]
  24. Rueter P., Rabus R., Wilkes H., Aeckersberg F., Rainey F. A., Jannasch H. W., Widdel F. 1994; Anaerobic oxidation of hydrocarbons in crude oil by new types of sulfate-reducing bacteria. Nature 372:455–458 [CrossRef]
    [Google Scholar]
  25. Schleifer K. H. 1985; Analysis of the chemical composition and primary structure of murein. Methods Microbiol 18:123–156
    [Google Scholar]
  26. Schleifer K. H., Kandler O. 1972; Peptidoglycan types of bacterial cell walls and their taxonomic implications. Bacteriol Rev 36:407–477
    [Google Scholar]
  27. Schumann P., Rainey F. A., Burghardt J., Stackebrandt E., Weiss N. 1999; Reclassification of Brevibacterium oxydans (Chatelain and Second 1966) as Microbacterium oxydans comb. nov. Int J Syst Bacteriol 49:175–177 [CrossRef]
    [Google Scholar]
  28. Takeuchi M., Hatano K. 1998a; Union of the genera Microbacterium Orla-Jensen and Aureobacterium Collins et al . in a redefined genus Microbacterium . Int J Syst Bacteriol 48:739–747 [CrossRef]
    [Google Scholar]
  29. Takeuchi M., Hatano K. 1998b; Proposal of six new species in the genus Microbacterium and transfer of Flavobacterium marinotypicum ZoBell and Upham to the genus Microbacterium as Microbacterium maritypicum comb. nov. Int J Syst Bacteriol 48:973–982 [CrossRef]
    [Google Scholar]
  30. Uchida K., Kudo T., Suzuki K., Nakase T. 1999; A new rapid method of glycolate test by diethyl ether extraction, which is applicable to a small amount of bacterial cells of less than one milligram. J Gen Appl Microbiol 45:49–56 [CrossRef]
    [Google Scholar]
  31. Van Hamme J. D., Singh A., Ward O. P. 2003; Recent advances in petroleum microbiology. Microbiol Mol Biol Rev 67:503–549 [CrossRef]
    [Google Scholar]
  32. Yokota A., Takeuchi M., Sakane T., Weiss N. 1993; Proposal of six new species in the genus Aureobacterium and transfer of Flavobacterium esteraromaticum Omelianski to the genus Aureobacterium as Aureobacterium esteraromaticum comb. nov. Int J Syst Bacteriol 43:555–564 [CrossRef]
    [Google Scholar]
  33. Zengler K., Richnow H. H., Rosselló-Mora R, Michaelis W, Widdel F. 1999; Methane formation from long-chain alkanes by anaerobic microorganisms. Nature 401:266–269 [CrossRef]
    [Google Scholar]
  34. Zlamala C., Schumann P., Kämpfer P., Valens M., Rosselló-Mora R., Lubitz W, Busse H.-J. 2002; Microbacterium aerolatum sp. nov., isolated from the air in the ‘Virgilkapelle’ in Vienna. Int J Syst Bacteriol 52:1229–1234 [CrossRef]
    [Google Scholar]
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