sp. nov., isolated from a mineral pool in Campania, Italy Free

Abstract

A haloalkaliphilic Gram-positive bacterium, strain 6AG, that grew aerobically at an optimum temperature of 37 °C and at pH 7–10 (optimum 9·0), was isolated from algal mat from a mineral pool located in Malvizza in the Campania region (southern Italy). The isolate tolerated high concentrations of NaCl, up to 25 %. On the basis of 16S rRNA gene sequence similarity, the strain was shown to belong to the genus . Analysis of the 16S rRNA gene sequence revealed high similarity between strain 6AG and an unidentified isolate from Hailaer soda lake (China) (99·9 % identity) and two Kenyan isolates, 3E1 and WE4 (98·3 and 97·8 % identity, respectively). The G+C content of the DNA was 48·4 mol%. The predominant respiratory quinones were MK-7(H), MK-7(H) and DMK-7(H); phosphatidylglycerol and diphosphatidylglycerol were the predominant polar lipids. iC15 : 0 and aiC15 : 0 were the major fatty acids. Strain 6AG accumulated osmolytes. The phylogenetic distance of strain 6AG (=DSM 15402=ATCC BAA-957) from any recognized species within the genus allowed it to be classified as the type strain of sp. nov.

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2005-01-01
2024-03-28
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References

  1. Arahal D. R., Ventosa A. 2002; Moderately halophilic and halotolerant species of Bacillus and related genera. In Applications and Systematics of Bacillus and Relatives pp  83–99 Edited by Berkeley R. C. W., Heyndrickx M., Logan N., Vos P. De. Oxford: Blackwell;
    [Google Scholar]
  2. Berkeley R. C. W., Heyndrickx M., Logan N., De Vos P. (editors) 2002 Applications and Systematics of Bacillus and Relatives Oxford: Blackwell;
    [Google Scholar]
  3. Chenna R., Sugawara H., Koike T., Lopez R., Gibson T. J., Higgins D. G., Thompson J. D. 2003; Multiple sequence alignment with the clustal series of programs. Nucleic Acids Res 31:3497–3500 [CrossRef]
    [Google Scholar]
  4. Duckworth A. W., Grant W. D., Jones B. E., van Steenbergen R. 1996; Phylogenetic diversity of soda lake alkaliphiles. FEMS Microbiol Ecol 19:181–191 [CrossRef]
    [Google Scholar]
  5. Dussault H. P. 1955; An improved technique for staining red halophilic bacteria. J Bacteriol 70:484–485
    [Google Scholar]
  6. Felsenstein J. 1993 phylip (phylogeny inference package), version 3.5c. Distributed by the author Department of Genetics, University of Washington; Seattle, USA:
    [Google Scholar]
  7. Fritze D. 1996; Bacillus haloalkaliphilus sp. nov. Int J Syst Bacteriol 46:98–101 [CrossRef]
    [Google Scholar]
  8. Halebian S., Harris B., Finegold S. M., Rolfe R. D. 1981; Rapid method that aids in distinguishing Gram-positive from Gram-negative anaerobic bacteria. J Clin Microbiol 13:444–448
    [Google Scholar]
  9. Nielsen P., Fritze D., Priest F. G. 1995; Phenetic diversity of alkaliphilic Bacillus strains: proposal for nine new species. Microbiology 141:1745–1761 [CrossRef]
    [Google Scholar]
  10. Oren A. 2002 Halophilic Microorganisms and Their Environments Boston: Kluwer Academic;
    [Google Scholar]
  11. Oren A., Galinski E. A. 1994; Hydrolysis of N′-benzoyl-arginine- p -nitroanilide stereoisomers as a phenotypic test: a study of Gram-positive halotolerant bacteria. Syst Appl Microbiol 17:7–10 [CrossRef]
    [Google Scholar]
  12. Romano I., Manca M. C., Lama L., Nicolaus B., Gambacorta A. 1993; A method for antibiotic assay on Sulfolobales . Biotechnol Tech 7:439–440 [CrossRef]
    [Google Scholar]
  13. Romano I., Nicolaus B., Lama L., Manca M. C., Gambacorta A. 1996; Characterization of a haloalkaliphilic strictly aerobic bacterium, isolated from Pantelleria Island. Syst Appl Microbiol 19:326–333 [CrossRef]
    [Google Scholar]
  14. Romano I., Nicolaus B., Lama L., Trabasso D., Caracciolo G., Gambacorta A. 2001; Accumulation of osmoprotectants and lipid pattern modulation in response to growth conditions by Halomonas pantelleriense . Syst Appl Microbiol 24:342–352 [CrossRef]
    [Google Scholar]
  15. Romano I., Giordano A., Lama L., Nicolaus B., Gambacorta A. 2003; Planococcus rifietensis sp. nov, isolated from algal mat collected from a sulfurous spring in Campania (Italy). Syst Appl Microbiol 26:357–366 [CrossRef]
    [Google Scholar]
  16. Saitou N., Nei M. 1987; The neighbor-joining method: a new method for reconstructing phylogenetic trees. Mol Biol Evol 4:406–425
    [Google Scholar]
  17. Tamaoka J., Komagata K. 1984; Determination of DNA base composition by reversed-phase high-performance liquid chromatography. FEMS Microbiol Lett 25:125–128 [CrossRef]
    [Google Scholar]
  18. Weisser J., Trüper H. G. 1985; Osmoregulation in a new haloalkaliphilic Bacillus from the Wadi Natrun (Egypt. ) Syst Appl Microbiol 6:7–11 [CrossRef]
    [Google Scholar]
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