1887

Abstract

Highly diverse strains nodulate genistoid legumes (brooms) in the Canary Islands, Morocco, Spain and the Americas. Phylogenetic analyses of ITS, , and sequences revealed that these isolates represent at least four distinct evolutionary lineages within the genus, namely and three unnamed genospecies. DNA–DNA hybridization experiments confirmed that one of the latter represents a new taxonomic species for which the name is proposed. populations experience homologous recombination at housekeeping loci, but are sexually isolated from sympatric bv. strains in soils of the Canary Islands. strains are highly acid-tolerant, nodulate diverse legumes in the tribes Genisteae and Loteae, but not species, whereas acid-sensitive soybean isolates such as USDA 6 and USDA 110 do not nodulate genistoid legumes. Based on host-range experiments and phylogenetic analyses of symbiotic and sequences, the biovarieties and for the genistoid legume and soybean isolates, respectively, were proposed. bv. strains display an overlapped host range with bv. isolates, both sharing monophyletic and alleles, possibly due to the lateral transfer of a conjugative chromosomal symbiotic island across species. is the sister species of , as inferred from a maximum-likelihood species phylogeny estimated from congruent + sequence partitions, which resolves eight species clades. In addition to the currently described species, this phylogeny uncovered the novel genospecies alpha and beta and the photosynthetic strains as independent evolutionary lineages. The type strain for is BTA-1 (=ATCC BAA-1002=LMG 22265=CFNE 1008).

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2005-03-01
2019-10-20
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vol. , part 2, pp. 569–575

Sequence accession numbers for new sequences used and generated in this study, the figures discussed in the text and our final and concluding remarks are available to download. [PDF](500KB)



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