1887

Abstract

Three Gram-positive, moderately halophilic, heterotrophic bacterial strains were isolated from a neutral saline lake in the Xin-Jiang area of China. The strains, designated 8-2, W11-1 and 25-7, were motile, spore-forming, aerobic rods and contained -diaminopimelic acid in their cell walls. Their DNA G+C contents were 37·4, 37·2 and 39·9 mol%, respectively. The main fatty acids in the cellular membranes of these novel strains were C and C methyl-branched. No species with validly published names showed 16S rRNA gene sequence similarity of more than 95 % with respect to these novel isolates; the most closely related species was a halophilic denitrifier, (94·6 %). Polyphasic taxonomic studies revealed that these strains belong to the and are distantly related to other genera of the family. It is proposed that a new genus, , should be created, with (type strain, 25-7=AS 1.3565=JCM 12389) as the type species. Another species, , is also proposed, with 8-2 (=AS 1.3566=JCM 12390) as the type strain.

Loading

Article metrics loading...

/content/journal/ijsem/10.1099/ijs.0.63287-0
2005-03-01
2019-09-22
Loading full text...

Full text loading...

/deliver/fulltext/ijsem/55/2/ijs550949.html?itemId=/content/journal/ijsem/10.1099/ijs.0.63287-0&mimeType=html&fmt=ahah

References

  1. Arahal, D. R., Márquez, M. C., Volcani, B. E., Schleifer, K. H. & Ventosa, A. ( 1999; ). Bacillus marismortui sp. nov., a new moderately halophilic species from the Dead Sea. Int J Syst Bacteriol 49, 521–530.[CrossRef]
    [Google Scholar]
  2. Arahal, D. R., Márquez, M. C., Volcani, B. E., Schleifer, K. H. & Ventosa, A. ( 2000; ). Reclassification of Bacillus marismortui as Salibacillus marismortui comb. nov. Int J Syst Evol Microbiol 50, 1501–1503.[CrossRef]
    [Google Scholar]
  3. Ash, C., Farrow, J. A. E., Wallbanks, S. & Collins, M. D. ( 1991; ). Phylogenetic heterogeneity of the genus Bacillus revealed by comparative analysis of small-subunit-ribosomal RNA sequences. Lett Appl Microbiol 13, 202–206.
    [Google Scholar]
  4. Denariaz, G., Payne, W. J. & Le Gall, J. ( 1989; ). A halophilic denitrifier, Bacillus halodenitrificans sp. nov. Int J Syst Bacteriol 39, 145–151.[CrossRef]
    [Google Scholar]
  5. Ezaki, T., Hashimoto, Y. & Yabuuchi, E. ( 1989; ). Fluorometric deoxyribonucleic acid-deoxyribonucleic acid hybridization in microdilution wells as an alternative to membrane filter hybridization in which radioisotopes are used to determine genetic relatedness among bacterial strains. Int J Syst Bacteriol 39, 224–229.[CrossRef]
    [Google Scholar]
  6. Fritze, D. ( 1996; ). Bacillus haloalkaliphilus sp. nov. Int J Syst Bacteriol 46, 98–101.[CrossRef]
    [Google Scholar]
  7. Gerhardt, P., Murray, R. G. E., Costilow, R. N., Nester, E. W., Woods, W. A., Krieg, N. R. & Philips, G. B. ( 1981; ). Manual of Methods for General Bacteriology. Washington, DC: American Society for Microbiology.
  8. Goto, K., Omura, T., Hara, Y. & Sadaie, Y. ( 2000; ). Application of the partial 16S rDNA sequence as an index for rapid identification of species in the genus Bacillus. J Gen Appl Microbiol 46, 1–8.[CrossRef]
    [Google Scholar]
  9. Gregersen, T. ( 1978; ). Rapid method for distinction of Gram-negative from Gram-positive bacteria. Eur J Appl Microbiol Biotechnol 5, 123–127.[CrossRef]
    [Google Scholar]
  10. Heyndrickx, M., Lebbe, L., Kersters, K., De Vos, P., Forsyth, G. & Logan, N. A. ( 1998; ). Virgibacillus: a new genus to accommodate Bacillus pantothenticus (Proom and Knight 1950). Emended description of Virgibacillus pantothenticus. Int J Syst Bacteriol 48, 99–106.[CrossRef]
    [Google Scholar]
  11. Heyrman, J., Logan, N. A., Busse, H.-J., Balcaen, A., Lebbe, L., Rodriguez-Diaz, M., Swings, J. & De Vos, P. ( 2003; ). Virgibacillus carmonensis sp. nov., Virgibacillus necropolis sp. nov. and Virgibacillus picturae sp. nov., three novel species isolated from deteriorated mural paintings, transfer of the species of the genus Salibacillus to Virgibacillus, as Virgibacillus marismortui comb. nov. and Virgibacillus salexigens comb. nov., and emended description of the genus Virgibacillus. Int J Syst Evol Microbiol 53, 501–511.[CrossRef]
    [Google Scholar]
  12. Kämpfer, P. ( 1994; ). Limits and possibilities of total fatty acid analysis for classification and identification of Bacillus species. Syst Appl Microbiol 17, 86–98.[CrossRef]
    [Google Scholar]
  13. Kodaka, H., Armfield, A. Y., Lombard, G. L. & Dowell, V. R., Jr ( 1982; ). Practical procedure for demonstrating bacterial flagella. J Clin Microbiol 16, 948–952.
    [Google Scholar]
  14. Kushner, D. J. ( 1985; ). The Halobacteriaceae. In The Bacteria, a Treatise on Structure and Function, vol. 8, pp. 171–206. Edited by I. C. Gunsalus, C. R. Woese & R. S. Wolfe. San Diego: Academic Press.
  15. Lu, J., Nogi, Y. & Takami, H. ( 2001; ). Oceanobacillus iheyensis gen. nov., sp. nov., a deep-sea extremely halotolerant and alkaliphilic species isolated from a depth of 1050 m on the Iheya Ridge. FEMS Microbiol Lett 205, 291–297.[CrossRef]
    [Google Scholar]
  16. Manchester, L., Pot, B., Kersters, K. & Goodfellow, M. ( 1990; ). Classification of Streptomyces and Streptoverticillium species by numerical analysis of electrophoretic protein patterns. Syst Appl Microbiol 13, 333–337.[CrossRef]
    [Google Scholar]
  17. Mesbah, M., Premachandran, U. & Whitman, W. B. ( 1989; ). Precise measurement of the G+C content of deoxyribonucleic acid by high-performance liquid chromatography. Int J Syst Bacteriol 39, 159–167.[CrossRef]
    [Google Scholar]
  18. Ren, P.-G. & Zhou, P.-J. ( 2005; ). Tenuibacillus multivorans gen. nov., sp. nov., a novel moderately halophilic bacterium isolated from saline soil in Xin-Jiang, China. Int J Syst Evol Microbiol 55, 95–99.[CrossRef]
    [Google Scholar]
  19. Saitou, N. & Nei, M. ( 1987; ). The neighbor-joining method: a new method for reconstructing phylogenetic trees. Mol Biol Evol 4, 406–425.
    [Google Scholar]
  20. Sambrook, J., Fritsch, E. F. & Maniatis, T. ( 1989; ). Molecular Cloning: a Laboratory Manual, 2nd edn. Cold Spring Harbor, NY: Cold Spring Harbor Laboratory.
  21. Schleifer, K. H. & Kandler, O. ( 1972; ). Peptidoglycan types of bacterial cell walls and their taxonomic implications. Bacteriol Rev 36, 407–477.
    [Google Scholar]
  22. Schlesner, H., Lawson, P. A., Collins, M. D., Weiss, N., Wehmeyer, U., Völker, H. & Thomm, M. ( 2001; ). Filobacillus milensis gen. nov., sp. nov., a new halophilic spore-forming bacterium with Orn–d-Glu-type peptidoglycan. Int J Syst Evol Microbiol 51, 425–431.
    [Google Scholar]
  23. Smibert, R. M. & Krieg, N. R. ( 1981; ). Phenotypic characterization. In Manual of Methods for General Microbiology, pp. 611–654. Washington, DC: American Society for Microbiology.
  24. Spring, S., Ludwig, W., Marquez, M. C., Ventosa, A. & Schleifer, K. H. ( 1996; ). Halobacillus gen. nov., with descriptions of Halobacillus litoralis sp. nov., and Halobacillus trueperi sp. nov., and transfer of Sporosarcina halophila to Halobacillus halophilus comb. nov. Int J Syst Bacteriol 46, 492–496.[CrossRef]
    [Google Scholar]
  25. Stackebrandt, E. & Goebel, B. M. ( 1994; ). Taxonomic note: a place for DNA-DNA reassociation and 16S rRNA sequence analysis in the present species definition in bacteriology. Int J Syst Bacteriol 44, 846–849.[CrossRef]
    [Google Scholar]
  26. Ventosa, A., Quesada, E., Rodriguez-Valera, F., Ruiz-Berraquero, F. & Ramos-Cormenzana, A. ( 1982; ). Numerical taxonomy of moderately halophilic Gram-negative rods. J Gen Microbiol 128, 1959–1968.
    [Google Scholar]
  27. Ventosa, A., Garcia, M. T., Kamekura, M., Onishi, H. & Ruiz-Berraquero, F. ( 1989; ). Bacillus halophilus sp. nov., a moderately halophilic Bacillus species. Syst Appl Microbiol 12, 162–165.[CrossRef]
    [Google Scholar]
  28. Wainø, M., Tindall, B. J., Schumann, P. & Ingvorsen, K. ( 1999; ). Gracilibacillus gen. nov., with description of Gracilibacillus halotolerans gen. nov., sp. nov.; transfer of Bacillus dipsosauri to Gracilibacillus dipsosauri comb. nov., and Bacillus salexigens to the genus Salibacillus gen. nov., as Salibacillus salexigens comb. nov. Int J Syst Bacteriol 49, 821–831.[CrossRef]
    [Google Scholar]
  29. Xin, H., Itoh, T., Zhou, P., Suzuki, K. & Nakase, T. ( 2001; ). Natronobacterium nitratireducens sp. nov., a haloalkaliphilic archaeon isolated from a soda lake in China. Int J Syst Evol Microbiol 51, 1825–1829.[CrossRef]
    [Google Scholar]
  30. Yoon, J.-H., Weiss, N., Lee, K.-C., Lee, I.-S., Kang, K. H. & Park, Y.-H. ( 2001; ). Jeotgalibacillus alimentarius gen. nov., sp. nov., a novel bacterium isolated from jeotgal with l-lysine in the cell wall, and reclassification of Bacillus marinus Rüger 1983 as Marinibacillus marinus gen. nov., comb. nov. Int J Syst Bacteriol 51, 2087–2093.[CrossRef]
    [Google Scholar]
  31. Yoon, J.-H., Kang, K. H. & Park, Y.-H. ( 2002; ). Lentibacillus salicampi gen. nov., sp. nov., a moderately halophilic bacterium isolated from a salt field in Korea. Int J Syst Evol Microbiol 52, 2043–2048.[CrossRef]
    [Google Scholar]
  32. Yoon, J.-H., Kang, K. H. & Park, Y.-H. ( 2003; ). Halobacillus salinus sp. nov., isolated from a salt lake on the coast of the East Sea in Korea. Int J Syst Evol Microbiol 53, 687–693.[CrossRef]
    [Google Scholar]
  33. Yoon, J.-H., Oh, T.-K. & Park, Y.-H. ( 2004; ). Transfer of Bacillus halodenitrificans Denariaz et al. 1989 to the genus Virgibacillus as Virgibacillus halodenitrificans comb. nov. Int J Syst Evol Microbiol 54, 2163–2167.[CrossRef]
    [Google Scholar]
  34. Zhu, F., Wang, S. & Zhou, P. ( 2003; ). Flavobacterium xinjiangense sp. nov. and Flavobacterium omnivorum sp. nov., novel psychrophiles from the China No. 1 glacier. Int J Syst Evol Microbiol 53, 853–857.[CrossRef]
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/ijsem/10.1099/ijs.0.63287-0
Loading
/content/journal/ijsem/10.1099/ijs.0.63287-0
Loading

Data & Media loading...

vol. , part 2, pp. 949–953

The results of SDS-PAGE of whole-cell proteins of strains 8-2 and W11-1 and a table showing the fatty acid compositions of strains 25-7 , W11-1 and 8-2 are available to download. [PDF](90KB)



PDF

Most Cited This Month

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error