1887

Abstract

The prevalence of leptospirosis is generally high in domestic animals and rodents in Tanzania. Identification of isolates from cattle was carried out to establish prevalent serovars. Serological typing was done based on monoclonal antibodies and the standard cross-agglutination absorption test. Molecular typing involved pathogenic- and saprophytic-specific PCRs and a PCR specifically amplifying DNA from the species . DNA fingerprinting with primers derived from sequences of insertion elements IS and IS was carried out. Both serological and molecular characterization indicated that one of the isolates, coded RM1, represents a new serovar of the species of serogroup Icterohaemorrhagiae. The serovar name Sokoine is proposed for this new isolate.

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2006-03-01
2019-10-20
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References

  1. Boom, R., Gol, C. J., Salimans, M. M., Jansen, C. L., Wertheim-van Dillen, P. M. & van der Noordaa, J. ( 1990; ). Rapid and simple method for purification of nucleic acids. J Clin Microbiol 28, 495–503.
    [Google Scholar]
  2. Brenner, D. J., Kaufmann, A. F., Sulzer, K. R., Steigerwalt, A. G., Rogers, F. C. & Weyant, R. S. ( 1999; ). Further determination of DNA relatedness between serogroups and serovars in the family Leptospiraceae with a proposal for Leptospira alexanderi sp. nov. and four new Leptospira genomospecies. Int J Syst Bacteriol 49, 839–858.[CrossRef]
    [Google Scholar]
  3. Cole, J. R., Jr, Sulzer, C. R. & Pursell, A. R. ( 1973; ). Improved microtechnique for the leptospiral agglutination test. Appl Microbiol 25, 976–980.
    [Google Scholar]
  4. Dikken, H. & Kmety, E. ( 1978; ). Serological typing methods of leptospires. Methods Microbiol 11, 260–295.
    [Google Scholar]
  5. Faine, S. ( 1982; ). Guidelines for Leptospirosis Control. Geneva: WHO offset Publication 67.
  6. Faine, S., Adler, B., Bolin, C. & Perolat, P. ( 1999; ). Leptospira and Leptospirosis, 2nd edn. Melbourne: Medisci.
  7. Gravekamp, C., Van de Kemp, H., Franzen, M., Carrington, D., Schoone, G. J., Van Eys, G. J. J. M., Everard, C. O. R., Hartskeerl, R. A. & Terpstra, W. J. ( 1993; ). Detection of seven species of pathogenic leptospires by PCR using two sets of primers. J Gen Microbiol 139, 1691–1700.[CrossRef]
    [Google Scholar]
  8. Johnson, R. C. & Harris, V. G. ( 1967; ). Differentiation of pathogenic and saprophytic leptospires. I. Growth at low temperatures. J Bacteriol 94, 27–31.
    [Google Scholar]
  9. Johnson, R. C. & Rogers, P. ( 1964; ). Differentiation of pathogenic and saprophytic leptospires with 8-azaguanine. J Bacteriol 88, 1618–1623.
    [Google Scholar]
  10. Kmety, E. & Dikken, H. ( 1993; ). Classification of the Species Leptospira Interrogans and History of its Serovars. Groningen: University Press Groningen.
  11. Korver, H., Kolk, A. H. J., Vingerhoed, J., van Leeuwen, J. & Terpstra, W. J. ( 1988; ). Classification of serovars of the Icterohaemorrhagiae serogroup by monoclonal antibodies. Isr J Vet Med 44, 15–18.
    [Google Scholar]
  12. Machang'u, R. S., Mgode, G. & Mpanduji, D. ( 1997; ). Leptospirosis in animals and humans in selected areas of Tanzania. Belg J Zool 127, 97–104.
    [Google Scholar]
  13. Machang'u, R. S., Mgode, G., Asenga, J., Mhamphi, G., Hartskeerl, R., Goris, M., Cox, C., Weetjens, B. & Verhagen, R. ( 2003; ). Characterization of Leptospira isolates from captive giant pouched rats, Cricetomys gambianus. In Rats, Mice and People, Rodent Biology and Management, pp. 40–42. Edited by G. R. Singleton, L. A. Hinds, C. J. Krebs & M. D. Spratt. Canberra: Australian Centre for International Agricultural Research.
  14. Machang'u, R. S., Mgode, G. F., Assenga, J. & 7 other authors ( 2004; ). Serological and molecular characterization of Leptospira serovar Kenya from captive African giant pouched rats (Cricetomys gambianus) from Morogoro Tanzania. FEMS Immunol Med Microbiol 41, 117–121.[CrossRef]
    [Google Scholar]
  15. Murgia, R., Riquelme, N., Baranton, G. & Cinco, M. ( 1997; ). Oligonucleotides specific for pathogenic and saprophytic leptospira occuring in water. FEMS Microbiol Lett 148, 27–34.[CrossRef]
    [Google Scholar]
  16. Perolat, P., Chappel, R. J., Adler, B., Baranton, G., Bulach, D. M., Billinghurst, M. L., Letocart, M., Merien, F. & Serrano, M. S. ( 1998; ). Leptospira fainei sp. nov., isolated from pigs in Australia. Int J Syst Bacteriol 48, 851–858.[CrossRef]
    [Google Scholar]
  17. Stallman, N. D. ( 1987; ). International committee on systematic bacteriology subcommittee on the taxonomy of Leptospira. Minutes of the meeting, 5 and 6 September 1986, Manchester, England. Int J Syst Bacteriol 37, 472–473.[CrossRef]
    [Google Scholar]
  18. Zuerner, R. L. & Bolin, C. A. ( 1997; ). Differentiation of Leptospira interrogans isolates by IS1500 hybridization and PCR assays. J Clin Microbiol 35, 2612–2617.
    [Google Scholar]
  19. Zuerner, R. L., Alt, D. & Bolin, C. A. ( 1995; ). IS1533-based PCR assay for identification of Leptospira interrogans “sensu lato” serovars. J Clin Microbiol 33, 3284–3289.
    [Google Scholar]
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vol. , part 3, pp. 593 - 597

Determination of the pathogenic status of isolate RM1 (serovar Sokoine) by PCR

Pathogenic status of isolate RM1 (serovar Sokoine) established by PCR and generated using LP1 and a1190 primers for pathogenic leptospires

Serotyping of leptospires with mAbs

Serotyping of leptospires with mAbs. Comparison of histograms of RM1 isolate (serovar Sokoine) and reference serovar Ndambari with Icterohaemorrhagiae/Sarmin mAbs. [Single PDF file](37 KB)



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