1887

Abstract

Prévot 1938 and Nakamura . 1973 have long been considered similar in terms of their biological and biochemical properties, but their taxonomic positions have not been clarified by DNA–DNA hybridization studies or rigorous analysis of 16S rRNA genes. In the present study, DNA–DNA hybridization analysis revealed that strains DSM 599, DSM 600 and KZ 1544 shared 83·0–86·3 % DNA relatedness with DSM 2632. 16S rRNA gene sequence analysis showed that the strains also shared high identity with DSM 2632 (99·7, 99·3 and 99·8 % for DSM 599, DSM 600 and KZ 1544, respectively), implying that and are synonyms. In addition, alignment of the inferred amino acid sequences for phospholipase C (PLC) indicated 96·5 % identity between PLCs from and , but relatively low identity with other clostridial species. These results strongly suggest that the species and should be united, with the name having priority.

Keyword(s): PLC, phospholipase C
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2005-05-01
2024-04-23
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References

  1. Borriello S. P. 1980; Clostridial flora of the gastrointestinal tract in health and disease . PhD thesis University of London;
  2. Carbon P., Ehresmann C., Ehresmann B., Ebel J.-P. 1979; The complete nucleotide sequence of the ribosomal 16-S RNA from Escherichia coli . Experimental details and cistron heterogeneities. Eur J Biochem 100:399–410 [CrossRef]
    [Google Scholar]
  3. Cato E. P., Georgy W. L., Finegold S. M. 1986; Genus Clostridium Prazmowski 1880, 23AL . In Bergey's Manual of Systematic Bacteriology vol. 2 pp  1141–1200 Edited by Sneath P. H. A., Mair N. S., Sharpe M. E., Holt J. G. Baltimore: Williams & Wilkins;
    [Google Scholar]
  4. Clark G. C., Briggs D. C., Karasawa T. 10 other authors 2003; C lostridium absonum alpha-toxin: new insights into clostridial phospholipase C substrate binding and specificity. J Mol Biol 333:759–769 [CrossRef]
    [Google Scholar]
  5. Collins M. D., Lawson P. A., Willems A., Cordoba J. J., Fernandez-Garayzabal J., Garcia P., Cai J., Hippe H., Farrow J. A. 1994; The phylogeny of the genus Clostridium : proposal of five new genera and eleven new species combinations. Int J Syst Bacteriol 44:812–826 [CrossRef]
    [Google Scholar]
  6. East A. K., Thompson D. E., Collins M. D. 1992; Analysis of operons encoding 23S rRNA of Clostridium botulinum type A. J Bacteriol 174:8158–8162
    [Google Scholar]
  7. Ezaki T., Hashimoto Y., Yabuuchi E. 1989; Fluorometric deoxyribonucleic acid-deoxyribonucleic acid hybridization in microdilution wells as an alternative to membrane filter hybridization in which radioisotopes are used to determine genetic relatedness among bacterial strains. Int J Syst Bacteriol 39:224–229 [CrossRef]
    [Google Scholar]
  8. Hayase M., Mitsui N., Tamai K., Nakamura S., Nishida S. 1974; Isolation of Clostridium absonum and its cultural and biochemical properties. Infect Immun 9:15–19
    [Google Scholar]
  9. Holdeman L. V., Cato E. P., Moore W. E. C. 1977 Anaerobe Laboratory Manual , 4th edn. Blacksburg, VA: Anaerobe Laboratory, Virginia Polytechnic Institute and State University;
    [Google Scholar]
  10. Jepson M., Titball R. W. 2000; Structure and function of clostridial phospholipases C. Microbes Infect 2:1277–1284 [CrossRef]
    [Google Scholar]
  11. Karasawa T., Wang X., Maegawa T., Michiwa Y., Kita H., Miwa K., Nakamura S. 2003; Clostridium sordellii phospholipase C: gene cloning and comparison of enzymatic and biological activities with those of Clostridium perfringens and Clostridium bifermentans phospholipase C. Infect Immun 71:641–646 [CrossRef]
    [Google Scholar]
  12. Masaki T., Umehashi H., Miyazaki H., Takano M., Yamakawa K., Nakamura S. 1988; Clostridium absonum from gas gangrene. Jpn J Med Sci Biol 41:27–30 [CrossRef]
    [Google Scholar]
  13. Nakamura S., Shimamura T., Hayase M., Nishida S. 1973; Numerical taxonomy of saccharolytic clostridia, particularly Clostridium perfringens -like strains: descriptions of Clostridium absonum sp.n. and Clostridium paraperfringens . Int J Syst Bacteriol 23:419–429 [CrossRef]
    [Google Scholar]
  14. Nakamura S., Shimamura T., Hayashi H., Nishida S. 1975; Reinvestigation of the taxonomy of Clostridium bifermentans and Clostridium sordellii . J Med Microbiol 8:299–309 [CrossRef]
    [Google Scholar]
  15. Nakamura S., Yamakawa K., Hashimoto H., Nishida S. 1979; Isolation of Clostridium absonum from a case of gas gangrene. Microbiol Immunol 23:685–687 [CrossRef]
    [Google Scholar]
  16. Prévot A. R. 1938; Études de systématique bactérienne. IV. Critique de la conception actuelle du genre Clostridium . Ann Inst Pasteur (Paris 61:72–91 (in French
    [Google Scholar]
  17. Raue H. A., Rosner A., Planta R. J. 1977; Heterogeneity of the genes coding for 5S RNA in three related strains of the genus Bacillus . Mol Gen Genet 156:185–193
    [Google Scholar]
  18. Rodriguez E., Mar Gamboa M. D., Fernandez B. 1993; Mesophilic clostridia from soil of the central plateau of Costa Rica. Rev Biol Trop 41:365–369 (in Spanish
    [Google Scholar]
  19. Titball R. W. 1999; Membrane-damaging and cytotoxic phospholipases. In The Comprehensive Sourcebook of Bacterial Protein Toxins . , 2nd edn. pp  310–329 Edited by Alouf J. E., Freer J. H. London: Academic Press;
  20. Tsutsui K., Minami J., Matsushita O., Katayama S., Taniguchi Y., Nakamura S., Nishioka M., Okabe A. 1995; Phylogenetic analysis of phospholipase C genes from Clostridium perfringens types A to E and Clostridium novyi . J Bacteriol 177:7164–7170
    [Google Scholar]
  21. Wang X., Maegawa T., Karasawa T. 7 other authors 2000; Genetic analysis of type E botulinum toxin-producing Clostridium butyricum strains. Appl Environ Microbiol 66:4992–4997 [CrossRef]
    [Google Scholar]
  22. Willis A. T., Hobbs G. 1958; A medium for the identification of clostridia producing opalescence in egg-yolk emulsion. J Pathol Bacteriol 75:299–305 [CrossRef]
    [Google Scholar]
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