1887

Abstract

A polyphasic taxonomic study of 14 isolates recovered from various human and veterinary clinical samples was performed. Phenotypically these isolates shared several characteristics with members of the and related genera. Random amplified polymorphic DNA fingerprinting and whole-cell protein analysis suggested the presence of multiple genomic groups, which was confirmed by DNA–DNA hybridization experiments. 16S rRNA gene sequence analysis indicated that these isolates were related to the genera , , , , , , and and belonged to the family . Based on the results of the present study the organisms were classified in a novel genus, gen. nov. This genus comprises one named species, sp. nov. (type strain LMG 22250=CCUG 45225) and five currently unnamed genomic species. The DNA G+C content of members of the novel genus is between 54·0 and 57·7 mol%.

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2005-01-01
2019-10-14
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References

  1. Blümel, S., Mark, B., Busse, H.-J., Kämpfer, P. & Stolz, A. ( 2001; ). Pigmentiphaga kullae gen. nov., sp. nov., a novel member of the family Alcaligenaceae with the ability to decolorize azo dyes aerobically. Int J Syst Evol Microbiol 51, 1867–1871.[CrossRef]
    [Google Scholar]
  2. Coenye, T., Falsen, E., Vancanneyt, M., Hoste, B., Govan, J. R. W., Kersters, K. & Vandamme, P. ( 1999; ). Classification of some Alcaligenes faecalis-like isolates from the environment and human clinical samples as Ralstonia gilardii sp. nov. Int J Syst Bacteriol 49, 405–413.[CrossRef]
    [Google Scholar]
  3. Coenye, T., Spilker, T., Martin, A. & LiPuma, J. J. ( 2002; ). Assessment of genotyping methods for epidemiologic study of Burkholderia cepacia genomovar III. J Clin Microbiol 40, 3300–3307.[CrossRef]
    [Google Scholar]
  4. Coenye, T., Vancanneyt, M., Falsen, E., Swings, J. & Vandamme, P. ( 2003a; ). Achromobacter insolitus sp. nov. and Achromobacter spanius sp. nov., from human clinical samples. Int J Syst Evol Microbiol 53, 1819–1824.[CrossRef]
    [Google Scholar]
  5. Coenye, T., Vancanneyt, M., Cnockaert, M., Falsen, E., Swings, J. & Vandamme, P. ( 2003b; ). Kerstersia gyiorum gen. nov., sp. nov., a novel Alcaligenes faecalis-like organism isolated from human clinical samples, and reclassification of Alcaligenes denitrificans Rüger and Tan 1983 as Achromobacter denitrificans comb. nov. Int J Syst Evol Microbiol 53, 1825–1831.[CrossRef]
    [Google Scholar]
  6. De Ley, J., Segers, P., Kersters, K., Mannheim, W. & Lievens, A. ( 1986; ). Intra- and intergeneric similarities of the Bordetella ribosomal ribonucleic acid cistrons: proposal for a new family Alcaligenaceae. Int J Syst Bacteriol 36, 405–414.[CrossRef]
    [Google Scholar]
  7. Ezaki, T., Hashimoto, Y. & Yabuuchi, E. ( 1989; ). Fluorometric deoxyribonucleic acid–deoxyribonucleic acid hybridization in microdilution wells as an alternative to membrane filter hybridization in which radioisotopes are used to determine genetic relatedness among bacterial strains. Int J Syst Bacteriol 39, 224–229.[CrossRef]
    [Google Scholar]
  8. Jang, S. S., Donahue, J. M., Arata, A. B., Goris, J., Hansen, L. M., Earley, D. L., Vandamme, P. A. R., Timoney, P. J. & Hirsh, D. C. ( 2001; ). Taylorella asinigenitalis sp. nov., a bacterium isolated from the genital tract of male donkeys (Equus asinus). Int J Syst Evol Microbiol 51, 971–976.[CrossRef]
    [Google Scholar]
  9. Kersters, K. & De Ley, J. ( 1984; ). Genus Alcaligenes Castellani and Chalmers 1919, 936AL. In Bergey's Manual of Systematic Bacteriology, vol. 1, pp. 361–373. Edited by N. R. Krieg & J. G. Holt. Baltimore: Williams & Wilkins.
  10. Kilian, M. & Biberstein, E. L. ( 1984; ). Genus II. Haemophilus Winslow, Broadhurst, Buchanan, Krumwiede, Rogers and Smith 1917, 561AL. In Bergey's Manual of Systematic Bacteriology, vol. 1, pp. 558–569. Edited by N. R. Krieg & J. G. Holt. Baltimore: Williams & Wilkins.
  11. Mesbah, M., Premachandran, U. & Whitman, W. B. ( 1989; ). Precise measurement of G+C content of deoxyribonucleic acid by high-performance liquid chromatography. Int J Syst Bacteriol 39, 159–167.[CrossRef]
    [Google Scholar]
  12. Pitcher, D. G., Saunders, N. A. & Owen, R. J. ( 1989; ). Rapid extraction of bacterial genomic DNA with guanidium thiocyanate. Lett Appl Microbiol 8, 109–114.[CrossRef]
    [Google Scholar]
  13. Pittman, M. ( 1984; ). Genus Bordetella Moreno-Lopéz 1952, 179. In Bergey's Manual of Systematic Bacteriology, vol. 1, pp. 388–393. Edited by N. R. Krieg & J. G. Holt. Baltimore: Williams & Wilkins.
  14. Pot, B., Vandamme, P. & Kersters, K. ( 1994; ). Analysis of electrophoretic whole-organism protein fingerprints. In Chemical Methods in Prokaryotic Systematics, pp. 493–521. Edited by M. Goodfellow & A. G. O'Donnell. Chichester: Wiley.
  15. Rossau, R., Kersters, K., Falsen, E., Jantzen, E., Segers, P., Union, A., Nehls, L. & De Ley, J. ( 1987; ). Oligella, a new genus including Oligella urethralis comb. nov. (formerly Moraxella urethralis), and Oligella ureolytica sp. nov. (formerly CDC group IVe): relationship to Taylorella equigenitalis and related taxa. Int J Syst Bacteriol 37, 198–210.[CrossRef]
    [Google Scholar]
  16. Saitou, N. & Nei, M. ( 1987; ). The neighbor-joining method: a new method for reconstructing phylogenetic trees. Mol Biol Evol 4, 406–425.
    [Google Scholar]
  17. Stackebrandt, E. & Goebel, B. M. ( 1994; ). Taxonomic note: a place for DNA–DNA reassociation and 16S rRNA sequence analysis in the present species definition in bacteriology. Int J Syst Bacteriol 44, 846–849.[CrossRef]
    [Google Scholar]
  18. Vahlne, G. ( 1945; ). Serological typing of the colon bacteria. Acta Pathol Microbiol Scand Suppl 62, 1–127.
    [Google Scholar]
  19. Vandamme, P., Vancanneyt, M., Pot, B. & 11 other authors ( 1992; ). Polyphasic taxonomic study of the emended genus Arcobacter with Arcobacter butzleri comb. nov. and Arcobacter skirrowii sp. nov., an aerotolerant bacterium isolated from veterinary specimens. Int J Syst Bacteriol 42, 344–356.[CrossRef]
    [Google Scholar]
  20. Vandamme, P., Gillis, M., Vancanneyt, M., Hoste, B., Kersters, K. & Falsen, E. ( 1993; ). Moraxella lincolnii sp. nov., isolated from the human respiratory tract, and re-evaluation of the taxonomic position of Moraxella osloensis. Int J Syst Bacteriol 43, 474–481.[CrossRef]
    [Google Scholar]
  21. Vandamme, P., Segers, P., Ryll, M. & 8 other authors ( 1998; ). Pelistega europaea gen. nov., sp. nov., a bacterium associated with respiratory disease in pigeons: taxonomic structure and phylogenetic allocation. Int J Syst Bacteriol 48, 431–440.[CrossRef]
    [Google Scholar]
  22. von Witzingerode, F., Schattke, A., Siddiqui, R. A., Rösick, U., Göbel, U. B. & Gross, R. ( 2001; ). Bordetella petrii sp. nov., isolated from an anaerobic bioreactor, and emended description of the genus Bordetella. Int J Syst Evol Microbiol 51, 1257–1265.
    [Google Scholar]
  23. Willems, A., Gilhaus, H., Beer, W., Mietke, H., Gelderblom, H. R., Burghardt, B., Voigt, W. & Reissbrodt, R. ( 2002; ). Brackiella oedipodis gen. nov., sp. nov., Gram-negative, oxidase-positive rods that cause endocarditis of cotton-topped tamarin (Saguinus oedipus). Int J Syst Evol Microbiol 52, 179–186.
    [Google Scholar]
  24. Yabuuchi, E., Kawamura, Y., Kosako, Y. & Ezaki, T. ( 1998; ). Emendation of the genus Achromobacter and Achromobacter xylosoxidans (Yabuuchi and Yano) and proposal of Achromobacter ruhlandii (Packer and Vishniac) comb. nov., Achromobacter piechaudii (Kiredjian et al.) comb. nov., and Achromobacter xylosoxidans subsp. denitrificans (Rüger and Tan) comb. nov. Microbiol Immunol 42, 429–438.[CrossRef]
    [Google Scholar]
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vol. , part 1, pp. 251 - 256

Protein profiles of the strains studied.

Fatty acid compositions of the strains studied.

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