1887

Abstract

Eight strains of with identical partial 16S rRNA gene sequences and similar randomly amplified polymorphic DNA patterns were isolated from fermentation samples from Japanese and Scottish malt whisky distilleries. Phylogenetic analysis of almost complete 16S rRNA gene sequences from three representative strains (two from Japan, one from Scotland) placed them in the genus as members of the group. and were the most closely related species, with 16S rRNA gene similarities of 99·3 and 98·1 %, respectively. A similar phylogeny was derived from partial sequences of elongation factor Tu () genes in which the alleles from the three distillery isolates were identical and shared 99·0 % similarity with and genes. S-layer () gene sequences suggested different relationships among the strains and the distillery isolates no longer formed a monophyletic group. The alleles from the Japanese and Scottish strains shared only 54 % similarity. Chromosomal DNA from the distillery strains gave DNA–DNA hybridization values between 79 and 100 % but showed less than 43 and 22 % reassociation with and DNA, respectively. The name sp. nov. is proposed for this novel taxon; the type strain is strain SA (=LMG 22464=NCIMB 14005).

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2005-01-01
2019-10-21
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References

  1. Bohak, I., Back, W., Richter, L., Ehrmann, M., Ludwig, W. & Schleifer, K. H. ( 1998; ). Lactobacillus amylolyticus sp. nov., isolated from beer malt and beer wort. Syst Appl Microbiol 21, 360–364.[CrossRef]
    [Google Scholar]
  2. Boot, H. J., Kolen, C. P., Pot, B., Kersters, K. & Pouwels, P. H. ( 1996; ). The presence of two S-layer-protein-encoding genes is conserved among species related to Lactobacillus acidophilus. Microbiology 142, 2375–2384.[CrossRef]
    [Google Scholar]
  3. Burton, K. ( 1968; ). Determination of DNA concentration with diphenylamine. Methods Enzymol 12, 163–166.
    [Google Scholar]
  4. Chavagnat, F., Haueter, M., Jimeno, J. & Casey, M. G. ( 2002; ). Comparison of partial tuf gene sequences for the identification of lactobacilli. FEMS Microbiol Lett 217, 177–183.[CrossRef]
    [Google Scholar]
  5. Du Plessis, E. M. & Dicks, L. M. ( 1995; ). Evaluation of random amplified polymorphic DNA (RAPD)-PCR as a method to differentiate Lactobacillus acidophilus, Lactobacillus crispatus, Lactobacillus amylovorus, Lactobacillus gallinarum, Lactobacillus gasseri and Lactobacillus johnsonii. Curr Microbiol 31, 114–118.[CrossRef]
    [Google Scholar]
  6. Falsen, E., Pascual, C., Sjödén, B., Ohlén, M. & Collins, M. D. ( 1999; ). Phenotypic and phylogenetic characterization of a novel Lactobacillus species from human sources: description of Lactobacillus iners sp. nov. Int J Syst Bacteriol 49, 217–221.[CrossRef]
    [Google Scholar]
  7. Fujisawa, T., Benno, Y., Yaeshima, T. & Mitsuoka, T. ( 1992; ). Taxonomic study of the Lactobacillus acidophilus group, with recognition of Lactobacillus gallinarum sp. nov. and Lactobacillus johnsonii sp. nov. and synonymy of Lactobacillus acidophilus group A3 ( Johnson et al. 1980 ) with the type strain of Lactobacillus amylovorus (Nakamura 1981). Int J Syst Bacteriol 42, 487–491.[CrossRef]
    [Google Scholar]
  8. Gancheva, A., Pot, B., Vanhonacker, K., Hoste, B. & Kersters, K. ( 1999; ). A polyphasic approach towards the identification of strains belonging to Lactobacillus acidophilus and related species. Syst Appl Microbiol 22, 573–585.[CrossRef]
    [Google Scholar]
  9. Horie, M., Kajikawa, H. S. & Toba, T. ( 2002; ). Identification of Lactobacillus crispatus by polymerase chain reaction targeting S-layer protein gene. Lett Appl Microbiol 35, 57–61.[CrossRef]
    [Google Scholar]
  10. Johnson, J. L., Phelps, C. F., Cummins, C. S., London, J. & Gasser, F. ( 1980; ). Taxonomy of the Lactobacillus acidophilus group. Int J Syst Bacteriol 30, 53–68.[CrossRef]
    [Google Scholar]
  11. Makanjuola, D. B. & Springham, D. G. ( 1984; ). Identification of lactic acid bacteria isolated from different stages of the Scotch whisky fermentation. J Inst Brew 90, 13–19.[CrossRef]
    [Google Scholar]
  12. Mukai, T., Arihara, K., Ikeda, A., Nomura, K., Suzuki, F. & Ohori, H. ( 2003; ). Lactobacillus kitasatonis sp. nov., from chicken intestine. Int J Syst Evol Microbiol 53, 2055–2059.[CrossRef]
    [Google Scholar]
  13. Simpson, K. L., Pettersson, B. & Priest, F. G. ( 2001; ). Characterization of lactobacilli from Scotch malt whisky distilleries and description of Lactobacillus ferintoshensis sp. nov., a new species isolated from malt whisky fermentations. Microbiology 147, 1007–1016.
    [Google Scholar]
  14. Takatani, T. & Ikemoto, H. ( 2004; ). Contribution of bacterial microflora in malt whisky quality. In Distilled Spirits: Tradition and Innovation, pp. 197–207. Edited by J. H. Bryce & G. G. Stewart. Nottingham: Nottingham University Press.
  15. van Beek, S. & Priest, F. G. ( 2002; ). Evolution of the lactic acid bacterial community during malt whisky fermentation: a polyphasic study. Appl Environ Microbiol 68, 297–305.[CrossRef]
    [Google Scholar]
  16. van Beek, S. & Priest, F. G. ( 2003; ). Bacterial diversity in Scotch whisky fermentations as revealed by denaturing gradient gel electrophoresis. J Am Soc Brew Chem 61, 10–14.
    [Google Scholar]
  17. van Loo, I. H. M., Heuvelman, K. J., King, A. J. & Mooi, F. R. ( 2002; ). Multilocus sequence typing of Bordetella pertussis based on surface protein genes. J Clin Microbiol 40, 1994–2001.[CrossRef]
    [Google Scholar]
  18. Ventura, M. & Zink, R. ( 2002; ). Specific identification and molecular typing analysis of Lactobacillus johnsonii by using PCR-based methods and pulsed-field gel electrophoresis. FEMS Microbiol Lett 217, 141–154.[CrossRef]
    [Google Scholar]
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RAPD profiles for distillery isolates and phylogenetically close relatives. Lanes: M, 100 bp marker; 1, DSM 10532 ; 2, Y10; 3, SA ; 4, M4; 5, NCIMB 11971 .

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Neighbour-joining tree showing the relationships of the distillery isolates with reference strains of the group based on partial gene sequences. was used as an outgroup. Bootstrap values (expressed as percentages of 1000 replications) are shown at branch points. Sequences generated in this study are indicated in bold. [PDF](11 KB)

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