sp. nov., a novel -aminoacylase-producing bacterium isolated from soil in Natori, Japan Free

Abstract

A rod-shaped, Gram-positive bacterium, strain TNJL143-2, having -acyl--amino acid amidohydrolase (-aminoacylase) activity, was isolated from a soil sample from Natori, Japan. It was a non-spore-forming, strictly aerobic bacterium without motility, showing a temperature optimum for growth of 30 °C and a pH optimum for growth of 5–7. The 16S rRNA gene sequence of the strain showed the highest similarities to members of the genus , in particular, , and . The chemotaxonomic characteristics, including the compositions of cellular menaquinones, cellular fatty acids and cell-wall amino acids, were consistent with those described for the genus . The G+C content of the genomic DNA was determined as 69·1 mol%. DNA–DNA hybridization studies using type strains of , and showed only low levels of relatedness (11–12 %). On the basis of these phenotypic and genotypic results, a novel species, sp. nov., is proposed, with TNJL143-2 (=JCM 12611=ATCC BAA-1032) as the type strain.

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2005-03-01
2024-03-28
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