1887

Abstract

Two marine, Gram-negative, aerobic, halophilic strains, designated KMM 3657 and KMM 3840, were isolated and found to be phylogenetically closely related to each other, showing 96·6 % 16S rRNA gene sequence similarity. Both strains are members of the genus in the - (94·7–98·0 % 16S rRNA gene sequence similarity). Strain KMM 3657 and SM19 were closely related, with 98·0 % sequence similarity. The novel strains shared generic physiological and chemotaxonomic properties with species, but differed in their temperature range for growth, inability to grow in 20 % NaCl and at >43 °C, metabolic properties and fatty acid composition. On the basis of phenotypic and phylogenetic analysis data, it is proposed that the strains represent two novel species, sp. nov., with the type strain KMM 3840 (=50-11=DSM 15401), and sp. nov., with the type strain KMM 3657 (=R65=DSM 15400).

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2005-01-01
2024-03-28
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References

  1. Allerberger F., Fritschel S. J. 1999; Use of automated ribotyping of Austrian Listeria monocytogenes isolates to support epidemiological typing. J Microbiol Methods 35:237–244 [CrossRef]
    [Google Scholar]
  2. DeSoete G. 1983; A least squares algorithm for fitting additive trees to proximity data. Psychometrika 48:621–626 [CrossRef]
    [Google Scholar]
  3. Felsenstein J. 1993 phylip (phylogeny inference package), version 3.5.1 Department of Genetics, University of Washington; Seattle, USA:
    [Google Scholar]
  4. Gauthier M. J., Lafay B., Christen R., Fernandez L., Acquaviva M., Bonin P., Bertrand J.-C. 1992; Marinobacter hydrocarbonoclasticus gen. nov., sp. nov., a new, extremely halotolerant, hydrocarbon-degrading marine bacterium. Int J Syst Bacteriol 42:568–576 [CrossRef]
    [Google Scholar]
  5. Gorshkova N. M., Ivanova E. P., Sergeev A. F., Zhukova N. V., Alexeeva Y., Wright J. P., Nicolau D. V., Mikhailov V. V., Christen R. 2003; Marinobacter excellens sp. nov., isolated from sediments of the Sea of Japan. Int J Syst Evol Microbiol 53:2073–2078 [CrossRef]
    [Google Scholar]
  6. Jukes T. H., Cantor C. R. 1969; Evolution of protein molecules. In Mammalian Protein Metabolism vol 3 pp  21–132 Edited by Munro H. N. New York: Academic Press;
    [Google Scholar]
  7. Leifson E. 1963; Determination of carbohydrate metabolism of marine bacteria. J Bacteriol 85:1183–1184
    [Google Scholar]
  8. Marmur J., Doty P. 1962; Determination of the base composition of deoxyribonucleic acid from its thermal denaturation temperature. J Mol Biol 4:109–118
    [Google Scholar]
  9. Martín S., Márquez M. C., Sánchez-Porro C., Mellodo E., Arahal D. R., Ventosa A. 2003; Marinobacter lipolyticus sp. nov., a novel moderate halophile with lipolytic activity. Int J Syst Evol Microbiol 53:1383–1387 [CrossRef]
    [Google Scholar]
  10. Nguyen B. H., Denner E. B. M., Dang T. C. H., Wanner G., Stan-Lotter H. 1999; Marinobacter aquaeolei sp. nov., a halophilic bacterium isolated from a Vietnamese oil-producing well. Int J Syst Bacteriol 49:367–375 [CrossRef]
    [Google Scholar]
  11. Owen R. J., Hill L. R., Lapage S. P. 1969; Determination of DNA base composition from melting profiles in dilute buffers. Biopolymers 7:503–516 [CrossRef]
    [Google Scholar]
  12. Rainey F. A., Ward-Rainey N., Kroppenstedt R. M., Stackebrandt E. 1996; The genus Nocardiopsis represents a phylogenetically coherent taxon and a distinct actinomycete lineage: proposal of Nocardiopsaceae fam. nov. Int J Syst Bacteriol 46:1088–1092 [CrossRef]
    [Google Scholar]
  13. Shieh W. Y., Jean W. D., Lin Y. T., Tseng M. 2003; Marinobacter lutaoensis sp. nov., a thermotolerant marine bacterium isolated from a coastal hot spring in Lutao, Taiwan. Can J Microbiol 49:244–252 [CrossRef]
    [Google Scholar]
  14. Smibert R. M., Krieg N. R. 1994; Phenotypic characterization. In Methods for General and Molecular Bacteriology pp  607–654 Edited by Gerhardt P., Murray R. G. E., Wood W. A., Krieg N. R. Washington, DC: American Society for Microbiology;
    [Google Scholar]
  15. Spröer C., Lang E., Hobeck P., Burghardt J., Stackebrandt E., Tindall B. J. 1998; Transfer of Pseudomonas nautica to Marinobacter hydrocarbonoclasticus . Int J Syst Bacteriol 48:1445–1448 [CrossRef]
    [Google Scholar]
  16. Stackebrandt E., Goebel B. M. 1994; Taxonomic note: a place for DNA-DNA reassociation and 16S rRNA sequence analysis in the present species definition in bacteriology. Int J Syst Bacteriol 44:846–849 [CrossRef]
    [Google Scholar]
  17. Yoon J.-H., Shin D.-Y., Kim I.-G., Kang K. H., Park Y.-H. 2003; Marinobacter litoralis sp. nov., a moderately halophilic bacterium isolated from sea water from the East Sea in Korea. Int J Syst Evol Microbiol 53:563–568 [CrossRef]
    [Google Scholar]
  18. Yoon J.-H., Yeo S. H., Kim I.-G., Oh T. K. 2004; Marinobacter flavimaris sp. nov. and Marinobacter daepoensis sp. nov., slightly halophilic organisms isolated from sea water of the Yellow Sea in Korea. Int J Syst Evol Microbiol 54:1799–1803 [CrossRef]
    [Google Scholar]
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