In an investigation of the yeast biota involved in silage deterioration, a considerable number of strains belonging to Saccharomyces and related genera were isolated from aerobically deteriorating corn silage in Tochigi, Japan. Analysis of sequences of the internal transcribed spacer and the large-subunit rRNA gene D1/D2 domain and electrophoretic karyotyping indicated that two of the strains, NS 14T and NS 26, represent a novel species with close phylogenetic relationships to Kazachstania servazzii and Kazachstania unispora. It is proposed that the novel species be named Kazachstania aerobia sp. nov., with NS 14T (=AS 2.2384T=CBS 9918T) as the type strain.
BaiF.-Y.,
LiangH.-Y.,
JiaJ.-H.2000; Taxonomic relationships among the taxa in the Candida guilliermondii complex, as revealed by comparative electrophoretic karyotyping. Int J Syst Evol Microbiol 50:417–422[CrossRef]
BaiF.-Y.,
ZhaoJ.-H.,
TakashimaM.,
JiaJ.-H.,
BoekhoutT.,
NakaseT.2002; Reclassification of the Sporobolomyces roseus and Sporidiobolus pararoseus complexes, with the description of Sporobolomyces phaffii sp. nov. Int J Syst Evol Microbiol 52:2309–2314[CrossRef]
JamesS. A.,
CaiJ.,
RobertsI. N.,
CollinsM. D.1997; A phylogenetic analysis of the genus Saccharomyces based on 18S rRNA gene sequences: description of Saccharomyces kunashirensis sp.nov. and Saccharomyces martiniae sp. nov. Int J Syst Bacteriol 47:453–460[CrossRef]
KurtzmanC. P.2003; Phylogenetic circumscription of Saccharomyces , Kluyveromyces and other members of the Sacchomycetaceae, and the proposal of the new genera Lachancea ,Nakaseomyces , Naumovia , Vanderwaltozyma and Zygotorulaspora
. FEMS Yeast Res 4, 233–245 [CrossRef]
KurtzmanC. P.,
RobnettC. J.2003; Phylogenetic relationships among yeasts of the ‘ Saccharomyces complex’ determined from multigene sequence analyses. FEMS Yeast Res 3:417–432[CrossRef]
MakimuraK.,
MurayamaY. S.,
YamaguchiH.1994; Detection of a wide range of medically important fungi by the polymerase chain reaction. J Med Microbiol 40:358–364[CrossRef]
MikataK.,
Ueda-NishimuraK.,
HisatomiT.2001; Three new species of Saccharomyces sensu lato van der Walt from Yaku Island in Japan: Saccharomyces naganishii sp.nov., Saccharomyces humaticus sp. nov. and Saccharomyces yakushimaensis sp. nov. Int J Syst Evol Microbiol 51:2189–2198[CrossRef]
NakaseT.,
SuzukiM.1986; Bullera megalospora , a new species of yeast forming large ballistospores isolated from dead leaves of Oryza sativa , Miscanthus sinensis , and Sasa sp. in Japan. J Gen Appl Microbiol 32:225–240[CrossRef]
NaumovG. I.,
NaumovaE. S.,
LanttoR. A.,
LouisE. J.,
KorholaM.1992; Genetic homology between Saccharomyces cerevisiae and its sibling species S. paradoxus and S. bayanus : electrophoretic karyotypes. Yeast 8:599–612[CrossRef]
NaumovG. I.,
NaumovaE. S.,
KorholaM.1995; Karyotypic relationships among species of Saccharomyces sensu lato: S. castellii , S. dairensis , S. unisporus and S. servazzii
. Syst Appl Microbiol 18:103–108[CrossRef]
Vaughan-MartiniA.,
MartiniA.1998; Saccharomyces Meyen ex Reess. In The Yeasts, a Taxonomic Study , 4th edn. pp 358–371 Edited by
KurtzmanC. P.,
FellJ. W.
Amsterdam: Elsevier;
Vaughan-MartiniA.,
MartiniA.,
GardinaliG.1993; Electrophoretic karyotyping as a taxonomic tool in the genus Saccharomyces
. Antonie Van Leeuwenhoek 63:145–156[CrossRef]
YamadaY.,
KondoK.1973; Coenzyme Q system in the classification of the yeast genera Rhodotorula and Cryptococcus and the yeast-like genera Sporobolomyces and Rhodosporidium
. J Gen Appl Microbiol 19:59–77[CrossRef]
YarrowD.1998; Methods for the isolation, maintenance and identification of yeasts. In The Yeasts, a Taxonomic Study , 4th edn. pp 77–100 Edited by
KurtzmanC. P,
FellJ. W.
Amsterdam: Elsevier;