1887

Abstract

The 16S–23S rRNA gene internal transcribed spacer (ITS) regions of 11 reference strains of species, together with and , were analysed to examine interspecies relationships. Compared with the phylogenetic tree generated using 16S rRNA gene sequences, the resolution of the ITS sequence-based tree was higher, but species positioning and clustering were similar with both approaches. The recent separation of and into distinct species was confirmed by the ITS data. In addition, analysis of the ITS sequences of 24 clinical isolates of plus the type strain ATCC 25260 divided the sequences into two clusters, of which one was -fucosidase-positive (like the type strain) while the other was -fucosidase-negative. The latter resembled the previously studied unusual extra-oral isolates of ‘-like organisms' (PELOs) which could therefore be called ‘-like organisms' (PALOs), based on the genetic identification. Moreover, the proposal of -fucosidase-negative strains as a new species should also be considered.

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2005-03-01
2019-10-22
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Similarity matrix tables (.xls), alignment files (.aln) and multi-sequence files (.msf) for all sequences discussed in this article are available to download [ZIP](47KB). A figure [PDF](154KB) showing representative gel-electrophoretic patterns of species is also available.

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Similarity matrix tables (.xls), alignment files (.aln) and multi-sequence files (.msf) for all sequences discussed in this article are available to download [ZIP](47KB). A figure [PDF](154KB) showing representative gel-electrophoretic patterns of species is also available.

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