1887

Abstract

A Gram-negative, aerobic to microaerophilic rod was isolated from 10 m depths of the hypersaline, heliothermal and meromictic Ekho Lake (East Antarctica). The strain was oxidase- and catalase-positive, metabolized a variety of carboxylic acids and sugars and produced lipase. Cells had an absolute requirement for artificial sea water, which could not be replaced by NaCl. A large absorption band at 870 nm indicated production of bacteriochlorophyll . The predominant fatty acids of this organism were 16 : 0 and 18 : 17, with 3-OH 10 : 0, 16 : 17 and 18 : 0 in lower amounts. The main polar lipids were diphosphatidylglycerol, phosphatidylglycerol and phosphatidylcholine. Ubiquinone 10 was produced. The DNA G+C content was 67 mol%. 16S rRNA gene sequence comparisons indicated that the isolate represents a member of the clade within the -. The organism showed no particular relationship to any members of this clade but clustered on the periphery of the genera , and ‘’ and the species . Distinct morphological, physiological and genotypic differences to these previously described taxa supported the description of a new genus and a novel species, for which the name gen. nov., sp. nov. is proposed. The type strain is EL-88 (=DSM 11466=CECT 7023).

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2005-01-01
2019-10-15
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vol. , part 1, pp. 41–47

A figure showing characteristic absorbance peaks for strain EL-88 is available to download. [PDF](238KB)



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