1887

Abstract

Two actinomycete strains, LK2-10 and LK2-5, which produced single, non-motile spores, were isolated from peat swamp forest soil in Yala Province, Thailand. A polyphasic study was carried out to establish the taxonomic position of these strains. Morphological and chemotaxonomic characteristics of these strains coincided with those of the genus . Phylogenetic analysis using 16S rRNA gene sequences also indicated that these strains should be classified in the genus and clearly separated from their closest relative, DSM 43818. Furthermore, a combination of DNA–DNA hybridization results and physiological and biochemical properties indicated that these strains were distinguished from all recognized species. These strains therefore represent a novel species, for which the name sp. nov. is proposed. The type strain is LK2-10 (=JCM 12345=PCU 238=DSM 44814=TISTR 1531).

Loading

Article metrics loading...

/content/journal/ijsem/10.1099/ijs.0.63217-0
2005-01-01
2021-02-28
Loading full text...

Full text loading...

/deliver/fulltext/ijsem/55/1/ijs550417.html?itemId=/content/journal/ijsem/10.1099/ijs.0.63217-0&mimeType=html&fmt=ahah

References

  1. Arai T. 1975 Culture Media for Actinomycetes Tokyo: The Society for Actinomycetes Japan;
    [Google Scholar]
  2. Collins M. D., Pirouz T., Goodfellow M., Minnikin D. E. 1977; Distribution of menaquinones in actinomycetes and corynebacteria. J Gen Microbiol 100:221–230 [CrossRef]
    [Google Scholar]
  3. Ezaki T., Hashimoto Y., Yabuuchi E. 1989; Fluorometric deoxyribonucleic acid-deoxyribonucleic acid hybridization in microdilution wells as an alternative to membrane filter hybridization in which radioisotopes are used to determine genetic relatedness among bacterial strains. Int J Syst Bacteriol 39:224–229 [CrossRef]
    [Google Scholar]
  4. Felsenstein J. 1985; Confidence limits on phylogenies: an approach using the bootstrap. Evolution 39:783–791 [CrossRef]
    [Google Scholar]
  5. Gordon R. E., Barnett D. A., Handerhan J. E., Pang C. H.-N. 1974; Nocardia coeliaca , Nocardia autotrophica , and the nocardin strain. Int J Syst Bacteriol 24:54–63 [CrossRef]
    [Google Scholar]
  6. Horan A. C., Brodsky B. C. 1986; Micromonospora rosaria sp. nov., nom. rev., the rosaramicin producer. Int J Syst Bacteriol 36:478–480 [CrossRef]
    [Google Scholar]
  7. Kämpfer P., Kroppenstedt R. M. 1996; Numerical analysis of fatty acid patterns of coryneform bacteria and related taxa. Can J Microbiol 42:989–1005 [CrossRef]
    [Google Scholar]
  8. Kasai H., Tamura T., Harayama S. 2000; Intrageneric relationships among Micromonospora species deduced from gyrB -based phylogeny and DNA relatedness. Int J Syst Evol Microbiol 50:127–134 [CrossRef]
    [Google Scholar]
  9. Kawamoto I. 1989; Genus Micromonospora . In Bergey's Manual of Systematic Bacteriology vol 4 pp  2442–2450 Edited by Williams S. T., Sharpe M. E., Holt J. G. Baltimore: Williams & Wilkins;
    [Google Scholar]
  10. Kawamoto I., Oka T., Nara T. 1981; Cell wall composition of Micromonospora olivoasterospora , Micromonospora sagamiensis , and related organisms. J Bacteriol 146:527–534
    [Google Scholar]
  11. Kawamoto I., Yamamoto M., Nara T. 1983; Micromonospora olivasterospora sp. nov. Int J Syst Bacteriol 33:107–112 [CrossRef]
    [Google Scholar]
  12. Kluge A. G., Farris F. S. 1969; Quantitative phyletics and the evolution of anurans. Syst Zool 18:1–32 [CrossRef]
    [Google Scholar]
  13. Koch C., Kroppenstedt R. M., Stackebrandt E. 1996a; Intrageneric relationships of the actinomycete genus Micromonospora . Int J Syst Bacteriol 46:383–387 [CrossRef]
    [Google Scholar]
  14. Koch C., Kroppenstedt R. M., Rainey F. A., Stackebrandt E. 1996b; 16S ribosomal DNA analysis of the genera Micromonospora ,Actinoplanes , Catellatospora , Catenuloplanes , Couchioplanes , Dactylosporangium , and Pilimelia and emendation of the family Micromonosporaceae . Int J Syst Bacteriol 46:765–768 [CrossRef]
    [Google Scholar]
  15. Kroppenstedt R. M. 1985; Fatty acid and menaquinone analysis of actinomycetes and related organisms. In Chemical Methods in Bacterial Systematics pp  173–199 Edited by Goodfellow M., Minnikin D. E. London: Academic Press;
    [Google Scholar]
  16. Lechevalier M. P., Lechevalier H. A. 1970; Chemical composition as a criterion in the classification of aerobic actinomycetes. Int J Syst Bacteriol 20:435–443 [CrossRef]
    [Google Scholar]
  17. Lechevalier M. P., DeBièvre C., Lechevalier H. A. 1977; Chemotaxonomy of aerobic actinomycetes: phospholipid composition. Biochem Syst Ecol 5:249–260 [CrossRef]
    [Google Scholar]
  18. Lee S. D., Goodfellow M., Hah Y. C. 1999; A phylogenetic analysis of the genus Catellatospora based on 16S ribosomal DNA sequence, including transfer of Catellatospora matsumotoense to the genus Micromonospora as Micromonospora matsumotoense comb. nov. FEMS Microbiol Lett 178:349–354 [CrossRef]
    [Google Scholar]
  19. Mikami H., Ishida Y. 1983; Post-column fluorometric detection of reducing sugar in high-performance liquid chromatography using arginine. Bunseki Kagaku 32:E207–E210 [CrossRef]
    [Google Scholar]
  20. Minnikin D. E., O'Donnell A. G., Goodfellow M., Alderson G., Athalye M., Schaal A., Parlett J. H. 1984; An integrated procedure for the extraction of bacterial isoprenoid quinones and polar lipids. J Microbiol Methods 2:233–241 [CrossRef]
    [Google Scholar]
  21. Nakajima Y., Kitpreechavanich V., Suzuki K., Kudo T. 1999; Microbispora corallina sp. nov., a new species of the genus Microbispora isolated from Thai soil. Int J Syst Bacteriol 49:1761–1767 [CrossRef]
    [Google Scholar]
  22. Ørskov J. 1923 Investigations into the Morphology of the Ray Fungi Copenhagen: Levin and Munksgaard;
    [Google Scholar]
  23. Saitou N., Nei M. 1987; The neighbor-joining method: a new method for reconstructing phylogenetic trees. Mol Biol Evol 4:406–425
    [Google Scholar]
  24. Sasser M. 1990; Identification of bacteria by gas chromatography of cellular fatty acids . MIDI Technical Note 101: Newark, DE: MIDI;
    [Google Scholar]
  25. Schleifer K. H., Kandler O. 1972; Peptidoglycan types of bacterial cell walls and their taxonomic implications. Bacteriol Rev 36:407–477
    [Google Scholar]
  26. Shirling E. B., Gottlieb D. 1966; Methods for characterization of Streptomyces species. Int J Syst Bacteriol 16:313–340 [CrossRef]
    [Google Scholar]
  27. Skerman V. B. D., McGowan V., Sneath P. H. A. 1980; Approved lists of bacterial names. Int J Syst Bacteriol 30:225–420 [CrossRef]
    [Google Scholar]
  28. Staneck J. L., Roberts G. D. 1974; Simplified approach to identification of aerobic actinomycetes by thin-layer chromatography. Appl Microbiol 28:226–231
    [Google Scholar]
  29. Tamaoka J. 1994; Determination of DNA base composition. In Chemical Methods in Prokaryotic Systematics pp  463–470 Edited by Goodfellow M., O'Donnell A. G. Chichester: Wiley;
    [Google Scholar]
  30. Tamaoka J., Komagata K. 1984; Determination of DNA base composition by reversed-phase high-performance liquid chromatography. FEMS Microbiol Lett 25:125–128 [CrossRef]
    [Google Scholar]
  31. Thompson J. D., Gibson T. J., Plewniak F., Jeanmougin F., Higgins D. G. 1997; The clustal_x windows interface: flexible strategies for multiple sequence alignment aided by quality analysis tools. Nucleic Acids Res 25:4876–4882 [CrossRef]
    [Google Scholar]
  32. Tomita K., Hoshino Y., Ohkusa N., Tsuno T., Miyaki T. 1992; Micromonospora chersina sp. nov. Actinomycetologica 621–28 [CrossRef]
    [Google Scholar]
  33. Uchida K., Aida K. 1984; An improved method for the glycolate test for simple identification of the acyl type of bacterial cell walls. J Gen Appl Microbiol 30:131–134 [CrossRef]
    [Google Scholar]
  34. Williams S. T., Cross T. 1971; Actinomycetes. Methods Microbiol 4:295–334
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/ijsem/10.1099/ijs.0.63217-0
Loading
/content/journal/ijsem/10.1099/ijs.0.63217-0
Loading

Data & Media loading...

Most cited this month Most Cited RSS feed

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error