1887

Abstract

A pleomorphic, extremely halophilic archaeon (strain M6) was isolated from a sulfide- and sulfur-rich spring in south-western Oklahoma (USA). It formed small (0·8–1·0 mm), salmon pink, elevated colonies on agar medium. The strain grew in a wide range of NaCl concentrations (6 % to saturation) and required at least 1 mM Mg for growth. Strain M6 was able to reduce sulfur to sulfide anaerobically. 16S rRNA gene sequence analysis indicated that strain M6 belongs to the family , genus ; it showed 96·7–98·0 % similarity to other members of the genus with validly published names and 89 % similarity to , its closest relative outside the genus . Polar lipid analysis and DNA G+C content further supported placement of strain M6 in the genus . DNA–DNA hybridization values, as well as biochemical and physiological characterization, allowed strain M6 to be differentiated from other members of the genus . A novel species, sp. nov., is therefore proposed to accommodate the strain. The type strain is M6 (=JCM 12327=CCM 7217=DSM 16227=CIP 108334).

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2004-11-01
2024-10-04
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