1887

Abstract

A pleomorphic, extremely halophilic archaeon (strain M6) was isolated from a sulfide- and sulfur-rich spring in south-western Oklahoma (USA). It formed small (0·8–1·0 mm), salmon pink, elevated colonies on agar medium. The strain grew in a wide range of NaCl concentrations (6 % to saturation) and required at least 1 mM Mg for growth. Strain M6 was able to reduce sulfur to sulfide anaerobically. 16S rRNA gene sequence analysis indicated that strain M6 belongs to the family , genus ; it showed 96·7–98·0 % similarity to other members of the genus with validly published names and 89 % similarity to , its closest relative outside the genus . Polar lipid analysis and DNA G+C content further supported placement of strain M6 in the genus . DNA–DNA hybridization values, as well as biochemical and physiological characterization, allowed strain M6 to be differentiated from other members of the genus . A novel species, sp. nov., is therefore proposed to accommodate the strain. The type strain is M6 (=JCM 12327=CCM 7217=DSM 16227=CIP 108334).

Loading

Article metrics loading...

/content/journal/ijsem/10.1099/ijs.0.63211-0
2004-11-01
2021-02-27
Loading full text...

Full text loading...

/deliver/fulltext/ijsem/54/6/ijs542275.html?itemId=/content/journal/ijsem/10.1099/ijs.0.63211-0&mimeType=html&fmt=ahah

References

  1. Asker D., Ohta Y. 2002; Haloferax alexandrinus sp. nov., an extremely halophilic canthaxanthin-producing archaeon from a solar saltern in Alexandria (Egypt. Int J Syst Evol Microbiol 52:729–738 [CrossRef]
    [Google Scholar]
  2. Balch W. E., Wolfe R. S. 1976; New approach to the cultivation of methanogenic bacteria: 2-mercaptoethanesulfonic acid (HS-CoM)-dependent growth of Methanobacterium ruminantium in a pressurized atmosphere. Appl Environ Microbiol 32:781–791
    [Google Scholar]
  3. Bryant M. P. 1972; Commentary on the Hungate technique for culture of anaerobic bacteria. Am J Clin Nutr 25:1324–1328
    [Google Scholar]
  4. Elshahed M. S., Najar F. Z., Roe B. A., Oren A., Dewers T. A., Krumholz L. R. 2004; Survey of archaeal diversity reveals an abundance of halophilic Archaea in a low-salt, sulfide- and sulfur-rich spring. Appl Environ Microbiol 70:2230–2239 [CrossRef]
    [Google Scholar]
  5. Emerson D., Chauhan S., Oriel P., Breznak J. A. 1994; Haloferax sp. D1227, a halophilic archaeon capable of growth on aromatic compounds. Arch Microbiol 161:445–452 [CrossRef]
    [Google Scholar]
  6. Gerhardt P., Murray R. G. E., Wood W. A., Krieg N. R. (editors) 1994; Methods for General and Molecular Bacteriology . pp  227–248 Washington, DC: American Society for Microbiology;
  7. Grant W. D., Ross H. N. M. 1986; The ecology and taxonomy of halobacteria. FEMS Microbiol Rev 39:9–15 [CrossRef]
    [Google Scholar]
  8. Grant W. D., Kamekura M., McGenity T. J., Ventosa A. 2001; The order Halobacteriales . In Bergey's Manual of Systematic Bacteriology , 2nd edn. vol 1 pp  294–334 Edited by Boone D. R., Castenholz R. W. New York: Springer;
    [Google Scholar]
  9. Gutierrez M. C., Kamekura M., Holmes M. L., Dyall-Smith M. L., Ventosa A. 2002; Taxonomic characterization of Haloferax sp.(‘ H. alicantei ’) strain Aa 2.2: description of Haloferax lucentensis sp. nov. Extremophiles 6:479–483 [CrossRef]
    [Google Scholar]
  10. Juez G., Rodriguez-Valera F., Ventosa A., Kushner D. J. 1986; Haloarcula hispanica spec. nov. and Haloferax gibbonsii spec. nov., two new species of extremely halophilic archaebacteria. Syst Appl Microbiol 8:75–79 [CrossRef]
    [Google Scholar]
  11. McGenity T. J., Gemmell R. T., Grant W. D. 1998; Proposal of a new halobacterial genus Natrinema gen. nov., with two species Natrinema pellirubrum nom.nov. and Natrinema pallidum nom. nov. Int J Syst Bacteriol 48:1187–1196 [CrossRef]
    [Google Scholar]
  12. Mullakhanbhai M. F., Larsen H. 1975; Halobacterium volcanii spec. nov., a Dead Sea halobacterium with a moderate salt requirement. Arch Microbiol 104:207–214 [CrossRef]
    [Google Scholar]
  13. Munson M. A., Nedwell D. B., Embley T. M. 1997; Phylogenetic diversity of Archaea in sediment samples from a coastal salt marsh. Appl Environ Microbiol 63:4729–4733
    [Google Scholar]
  14. Oren A. 2000 The order Halobacteriales . In The Prokaryotes: an Evolving Electronic Resource for the Microbiological Community , 3rd edn, release 3.2. Edited by Dworkin M., Falkow S., Rosenberg E., Schleifer K.-H., Stackebrandt E. New York: Springer; http://141.150.157.117:8080/prokPUB/index.htm
    [Google Scholar]
  15. Oren A. 2002; Molecular ecology of extremely halophilic Archaea and Bacteria . FEMS Microbiol Ecol 39:1–7 [CrossRef]
    [Google Scholar]
  16. Oren A., Ventosa A., Grant W. D. 1997; Proposal of minimal standards for the description of new taxa in the order Halobacteriales . Int J Syst Bacteriol 47:233–238 [CrossRef]
    [Google Scholar]
  17. Reysenbach A. L., Longnecker K., Kirshtein J. 2000; Novel bacterial and archaeal lineages from an in situ growth chamber deployed at a Mid-Atlantic Ridge hydrothermal vent. Appl Environ Microbiol 66:3798–3806 [CrossRef]
    [Google Scholar]
  18. Rodriguez-Valera F., Ruiz-Berraquero F., Ramos-Cormenzana A. 1979; Isolation of extreme halophiles from seawater. Appl Environ Microbiol 38:164–165
    [Google Scholar]
  19. Rodriguez-Valera F., Juez G., Kushner D. J. 1983; Halobacterium mediterranei spec. nov., a new carbohydrate-utilizing extreme halophile. Syst Appl Microbiol 4:369–381 [CrossRef]
    [Google Scholar]
  20. Takai K., Komatsu T., Inagaki F., Horikoshi K. 2001; Distribution of archaea in a black smoker chimney structure. Appl Environ Microbiol 67:3618–3629 [CrossRef]
    [Google Scholar]
  21. Thompson J. D., Gibson T. J., Plewniak F., Jeanmougin F., Higgins D. G. 1997; The clustal_x windows interface: flexible strategies for multiple sequence alignment aided by quality analysis tools. Nucleic Acids Res 25:4876–4882 [CrossRef]
    [Google Scholar]
  22. Tindall B. J., Trüper H. G. 1986; Ecophysiology of the aerobic halophilic archaebacteria. Syst Appl Microbiol 7:202–212 [CrossRef]
    [Google Scholar]
  23. Tomlinson G. A., Jahnke L. L., Hochstein L. I. 1986; Halobacterium denitrificans sp. nov., an extremely halophilic denitrifying bacterium. Int J Syst Bacteriol 36:66–70 [CrossRef]
    [Google Scholar]
  24. Torreblanca M., Rodriguez-Valera F., Juez G., Ventosa A., Kamekura M., Kates M. 1986; Classification of non-alkaliphilic halobacteria based on numerical taxonomy and polar lipid composition, and description of Haloarcula gen.nov. and Haloferax gen. nov. Syst Appl Microbiol 8:89–99 [CrossRef]
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/ijsem/10.1099/ijs.0.63211-0
Loading
/content/journal/ijsem/10.1099/ijs.0.63211-0
Loading

Data & Media loading...

Supplements

Supplementary material 1

PDF

Supplementary material 2

PDF

Most cited this month Most Cited RSS feed

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error