1887

Abstract

Phylogenetic analysis of 16S rRNA gene sequences revealed that is not a member of the genus , but is related to species of the genus . On a phylogenetic tree, clustered with and , with which it showed the highest 16S rRNA gene sequence similarity level (about 94 %). Phenotypic studies indicated that was also unable to produce acid from lactose, providing further evidence of its affiliation to the genus . DNA hybridization studies indicated that was clearly a separate species, different from and (reassociation levels of about 23 and 54 %, respectively). As suggested in previous studies, is closely related to but clearly distinct from . Based upon properties that taxonomically distinguish it from species of the genus , it is proposed that be transferred to the genus and reclassified as comb. nov. (type strain, 885/78=ATCC 49428=CCUG 29293=CIP 103330=DSM 5634=JCM 8736=LMG 12890=NCTC 12193).

Loading

Article metrics loading...

/content/journal/ijsem/10.1099/ijs.0.63205-0
2005-03-01
2024-12-08
Loading full text...

Full text loading...

/deliver/fulltext/ijsem/55/2/ijs550589.html?itemId=/content/journal/ijsem/10.1099/ijs.0.63205-0&mimeType=html&fmt=ahah

References

  1. Barros R. R., Carvalho M. G. S., Peralta J. M., Facklam R. R., Teixeira L. M. 2001; Phenotypic and genotypic characterization of Pediococcus strains isolated from human clinical sources. J Clin Microbiol 39:1241–1246 [CrossRef]
    [Google Scholar]
  2. Buschelman B. J., Bale M. J., Jones R. N. 1993; Species identification and determination of high-level aminoglycoside resistance among enterococci. Comparison study of sterile body fluid isolates, 1985–1991. Diagn Microbiol Infect Dis 16119–122 [CrossRef]
    [Google Scholar]
  3. Cai Y., Kumai S., Ogawa M., Benno Y., Nakase T. 1999; Characterization and identification of Pediococcus species isolated from forage crops and their application for silage preparation. Appl Environ Microbiol 65:2901–2906
    [Google Scholar]
  4. Collins M. D., Facklam R. R., Farrow J. A. E., Williamson R. 1989; Enterococcus raffinosus sp. nov., Enterococcus solitarius sp. nov. and Enterococcus pseudoavium sp. nov. FEMS Microbiol Lett 48:283–288
    [Google Scholar]
  5. Collins M. D., Williams A. M., Wallbanks S. 1990; The phylogeny of Aerococcus and Pediococcus as determined by 16S rRNA sequences analysis: description of Tetragenococcus gen. nov. FEMS Microbiol Lett 70:255–262
    [Google Scholar]
  6. Ennahar S., Cai Y. 2004; Genetic evidence that Weissella kimchii Choi et al . 2002 is a later heterotypic synonym of Weissella cibaria Björkroth et al . 2002. Int J Syst Evol Microbiol 54:463–465 [CrossRef]
    [Google Scholar]
  7. Ennahar S., Cai Y., Fujita Y. 2003; Phylogenetic diversity of lactic acid bacteria associated with paddy rice silage as determined by 16S ribosomal DNA analysis. Appl Environ Microbiol 69:444–451 [CrossRef]
    [Google Scholar]
  8. Ezaki T., Hashimoto Y., Yabuuchi E. 1989; Fluorometric deoxyribonucleic acid-deoxyribonucleic acid hybridization in microdilution wells as an alternative to membrane filter hybridization in which radioisotopes are used to determine genetic relatedness among bacterial strains. Int J Syst Bacteriol 39:224–229 [CrossRef]
    [Google Scholar]
  9. Facklam R. R., Collins M. D. 1989; Identification of Enterococcus species isolated from human infections by a conventional test scheme. J Clin Microbiol 27:731–734
    [Google Scholar]
  10. Ke D., Picard F. J., Martineau F., Ménard C., Roy P. H., Ouellette M., Bergeron M. G. 1999; Development of a PCR assay for rapid detection of enterococci. J Clin Microbiol 37:3497–3503
    [Google Scholar]
  11. Monstein H. J., Ahrné S., Molin G., Nikpour-Badr S., Jonasson J. 2001; Identification of enterococcal isolates by temperature gradient gel electrophoresis and partial sequence analysis of PCR-amplified 16S rDNA variable V6 regions. APMIS 109:209–216 [CrossRef]
    [Google Scholar]
  12. Patel R., Piper K. E., Rouse M. S., Steckelberg J. M., Uhl J. R., Kohner P., Hopkins M. K., Cockerill F. R. III, Kline B. C. 1998; Determination of 16S rRNA sequences of enterococci and application to species identification of nonmotile Enterococcus gallinarum isolates. J Clin Microbiol 36:3399–3407
    [Google Scholar]
  13. Poyart C., Quesnes G., Trieu-Cuot P. 2000; Sequencing the gene encoding manganese-dependent superoxide dismutase for rapid species identification of enterococci. J Clin Microbiol 38:415–418
    [Google Scholar]
  14. Ruoff K. L., de la Maza L., Murtagh M. J., Spargo J. D., Ferraro M. J. 1990; Species identities of enterococci from clinical specimens. J Clin Microbiol 28:435–437
    [Google Scholar]
  15. Sambrook J., Fritsch E. F., Maniatis T. 1989 Molecular Cloning: a Laboratory Manual , 2nd edn. Cold Spring Harbor, NY: Cold Spring Harbor Laboratory;
    [Google Scholar]
  16. Satomi M., Kimura B., Mizoi M., Sato T., Fujii T. 1997; Tetragenococcus muriaticus sp. nov., a new moderately halophilic lactic acid bacterium isolated from fermented squid liver sauce. Int J Syst Bacteriol 47:832–836 [CrossRef]
    [Google Scholar]
  17. Tsakris A., Woodford N., Pournaras S., Kaufmann M., Douboyas J. 1998; Apparent increased prevalence of high-level aminoglycoside-resistant Enterococcus durans resulting from false identification by a semiautomated software. J Clin Microbiol 36:1419–1421
    [Google Scholar]
  18. Wayne L. G., Brenner D. J., Colwell R. R. 9 other authors 1987; International Committee on Systematic Bacteriology. Report of the ad hoc committee on reconciliation of approaches to bacterial systematics. Int J Syst Bacteriol 37:463–464 [CrossRef]
    [Google Scholar]
  19. Williams A. M., Rodrigues U. M., Collins M. D. 1991; Intragenic relationships of enterococci as determined by reverse transcriptase sequencing of small-subunit rRNA. Res Microbiol 142:67–74 [CrossRef]
    [Google Scholar]
/content/journal/ijsem/10.1099/ijs.0.63205-0
Loading
/content/journal/ijsem/10.1099/ijs.0.63205-0
Loading

Data & Media loading...

Supplements

Supplementary material 1

PDF
This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error