1887

Abstract

Three strains of , B90A, UT26 and Sp+, isolated from different geographical locations, were found to degrade hexachlorocyclohexane. Phylogenetic analysis based on 16S rRNA gene sequences indicated that these strains do not fall in a clade that includes the type strain, ATCC 29837, but form a coherent cluster with [] IMSNU 11152 followed by ATCC 33790. The three strains showed low DNA–DNA relatedness values with ATCC 29837 (8–25 %), [] IMSNU 11152 (10–17 %), ATCC 33790 (23–54 %) and DSM 6383 (10–28 %), indicating that they do not belong to any of these species. Although the three strains were found to be closely related to each other based on 16S rRNA gene sequence similarity (99·1–99·4 %), DNA–DNA relatedness (19–59 %) and pulsed-field gel electrophoresis (PFGE) patterns indicated that they possibly represent three novel species of the genus . The three strains could also be readily distinguished by biochemical tests. The three strains showed similar polar lipid profiles and contained sphingoglycolipids. The strains differed from each other in fatty acid composition but contained the predominant fatty acids characteristic of other species. A phylogenetic study based on 16S rRNA gene sequences showed that [] IMSNU 11152 formed a cluster with members of the genus . Based on these results, it is proposed that strains B90A, UT26 and Sp+, previously known as , are the type strains of sp. nov. (=MTCC 6364=CCM 7286), sp. nov. (=MTCC 6362=CCM 7287) and sp. nov. (=MTCC 6363=CCM 7288), respectively. It is also proposed that [] be transferred to comb. nov.

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2005-09-01
2019-08-23
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