1887

Abstract

Two Gram-negative, motile, non-spore-forming, rod-shaped organisms, strains SW-117 and SW-120, were isolated from sea water of the Yellow Sea in Korea and subjected to a polyphasic taxonomic study. Strains SW-117 and SW-120 simultaneously contained both menaquinones (MK) and ubiquinones (Q) as isoprenoid quinones; the predominant menaquinone was MK-7 and the predominant ubiquinones were Q-7 and Q-8. The major fatty acid detected in the two strains was iso-C. The DNA G+C content of strains SW-117 and SW-120 was 51 and 54 mol%, respectively. Phylogenetic analyses based on 16S rRNA gene sequences showed that strains SW-117 and SW-120 fall within the radiation of the cluster comprising species. Strains SW-117 and SW-120 showed a 16S rRNA gene sequence similarity of 97·4 % and a DNA–DNA relatedness level of 10·1 %. Strains SW-117 and SW-120 exhibited 16S rRNA gene sequence similarity levels of 93·8–98·5 % and 92·4–97·0 %, respectively, to species. Strain SW-117 exhibited DNA–DNA relatedness levels of 8·3–20·3 % to the type strains of six phylogenetically related species. On the basis of phenotypic, phylogenetic and genetic data, strains SW-117 and SW-120 were classified in the genus as two distinct novel species, for which the names sp. nov. (type strain, SW-117=KCCM 41822=JCM 12192) and sp. nov. (type strain, SW-120=KCCM 41821=JCM 12193) are proposed, respectively.

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2004-11-01
2021-03-02
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