1887

INTERNATIONAL JOURNAL OF SYSTEMATIC AND EVOLUTIONARY MICROBIOLOGY logo

Volume 54, Issue 6

sp. nov. and sp. nov., slightly halophilic organisms isolated from sea water of the Yellow Sea in Korea Free

Abstract

Two Gram-negative, motile, non-spore-forming, rod-shaped organisms, strains SW-117 and SW-120, were isolated from sea water of the Yellow Sea in Korea and subjected to a polyphasic taxonomic study. Strains SW-117 and SW-120 simultaneously contained both menaquinones (MK) and ubiquinones (Q) as isoprenoid quinones; the predominant menaquinone was MK-7 and the predominant ubiquinones were Q-7 and Q-8. The major fatty acid detected in the two strains was iso-C. The DNA G+C content of strains SW-117 and SW-120 was 51 and 54 mol%, respectively. Phylogenetic analyses based on 16S rRNA gene sequences showed that strains SW-117 and SW-120 fall within the radiation of the cluster comprising species. Strains SW-117 and SW-120 showed a 16S rRNA gene sequence similarity of 97·4 % and a DNA–DNA relatedness level of 10·1 %. Strains SW-117 and SW-120 exhibited 16S rRNA gene sequence similarity levels of 93·8–98·5 % and 92·4–97·0 %, respectively, to species. Strain SW-117 exhibited DNA–DNA relatedness levels of 8·3–20·3 % to the type strains of six phylogenetically related species. On the basis of phenotypic, phylogenetic and genetic data, strains SW-117 and SW-120 were classified in the genus as two distinct novel species, for which the names sp. nov. (type strain, SW-117=KCCM 41822=JCM 12192) and sp. nov. (type strain, SW-120=KCCM 41821=JCM 12193) are proposed, respectively.

  • Published Online:
SGM
Loading

Article metrics loading...

/content/journal/ijsem/10.1099/ijs.0.63198-0
2004-11-01
2024-04-18
Loading full text...

Full text loading...

/deliver/fulltext/ijsem/54/6/ijs542347.html?itemId=/content/journal/ijsem/10.1099/ijs.0.63198-0&mimeType=html&fmt=ahah

References

  1. Anzai Y., Kim H., Park J.-Y., Wakabayashi H., Oyaizu H. 2000; Phylogenetic affiliation of the pseudomonads based on 16S rRNA sequence. Int J Syst Evol Microbiol 50:1563–1589 [CrossRef]
     [Google Scholar]
  2. Baumann L., Baumann P. 1981; The marine Gram-negative eubacteria; genera Photobacterium , Beneckea , Alteromonas , Pseudomonas , and Alcaligenes . In The Prokaryotes . A Handbook on Habitats, Isolation, and Identification of Bacteria pp  1302–1330 Edited by Starr M. P., Stolp H., Trüper H. G., Balows A., Schlegel H. G. Berlin: Springer;
     [Google Scholar]
  3. Bowman J. P., McCammon S. A., Nichols D. S., Skerratt J. H., Rea S. M., Nichols P. D., McMeekin T. A. 1997; Shewanella gelidimarina sp. nov. and Shewanella frigidimarina sp. nov. novel Antarctic species with the ability to produce eicosapentaenoic acid (20 : 5 ω 3) and grow anaerobically by dissimilatory Fe(III) reduction. Int J Syst Bacteriol 47:1040–1047 [CrossRef]
     [Google Scholar]
  4. Bozal N., Montes M. J., Tudela E., Jiménez F., Guinea J. 2002; Shewanella frigidimarina and Shewanella livingstonensis sp. nov. isolated from Antarctic coastal areas. Int J Syst Evol Microbiol 52:195–205
     [Google Scholar]
  5. Brink A. J., van Straten A., van Rensburg A. J. 1995; Shewanella ( Pseudomonas ) putrefaciens bacteremia. Clin Infect Dis 20:1327–1332 [CrossRef]
     [Google Scholar]
  6. Bruns A., Rohde M., Berthe-Corti L. 2001; Muricauda ruestringensis gen. nov. sp. nov. a facultatively anaerobic, appendaged bacterium from German North Sea intertidal sediment. Int J Syst Evol Microbiol 511997–2006 [CrossRef]
     [Google Scholar]
  7. Cowan S. T., Steel K. J. 1965 Manual for the Identification of Medical Bacteria London: Cambridge University Press;
     [Google Scholar]
  8. Ezaki T., Hashimoto Y., Yabuuchi E. 1989; Fluorometric deoxyribonucleic acid-deoxyribonucleic acid hybridization in microdilution wells as an alternative to membrane filter hybridization in which radioisotopes are used to determine genetic relatedness among bacterial strains. Int J Syst Bacteriol 39:224–229 [CrossRef]
     [Google Scholar]
  9. Ivanova E. P., Sawabe T., Gorshkova N. M., Svetashev V. I., Mikhailov V. V., Nicolau D. V., Christen R. 2001; Shewanella japonica sp. nov. Int J Syst Evol Microbiol 51:1027–1033 [CrossRef]
     [Google Scholar]
  10. Ivanova E. P., Sawabe T., Hayashi K., Gorshkova N. M., Zhukova N. V., Nedashkovskaya O. I., Mikhailov V. V., Nicolau D. V., Christen R. 2003a; Shewanella fidelis sp. nov., isolated from sediments and sea water. Int J Syst Evol Microbiol 53:577–582 [CrossRef]
     [Google Scholar]
  11. Ivanova E. P., Nedashkovskaya O. I., Zhukova N. V., Nicolau D. V., Christen R., Mikhailov V. V. 2003b; Shewanella waksmanii sp. nov., isolated from a sipuncula ( Phascolosoma japonicum ). Int J Syst Evol Microbiol 53:1471–1477 [CrossRef]
     [Google Scholar]
  12. Ivanova E. P., Nedashkovskaya O. I., Sawabe T., Zhukova N. V., Frolova G. M., Nicolau D. V., Mikhailov V. V., Bowman J. P. 2004; Shewanella affinis sp. nov., isolated from marine invertebrates. Int J Syst Evol Microbiol 54:1089–1093 [CrossRef]
     [Google Scholar]
  13. Komagata K., Suzuki K.-I. 1987; Lipids and cell-wall analysis in bacterial systematics. Methods Microbiol 19:161–203
     [Google Scholar]
  14. Lanyi B. 1987; Classical and rapid identification methods for medically important bacteria. Methods Microbiol 19:1–67
     [Google Scholar]
  15. Leifson E. 1963; Determination of carbohydrate metabolism of marine bacteria. J Bacteriol 85:1183–1184
     [Google Scholar]
  16. Levring T. 1946; Some culture experiments with Ulva and artificial seawater. K Fysiogr Sällsk Lund Förh 16:45–56
     [Google Scholar]
  17. MacDonell M. T., Colwell R. R. 1985; Phylogeny of the Vibrionaceae , and recommendation for two new genera, Listonella and Shewanella . Syst Appl Microbiol 6:171–182 [CrossRef]
     [Google Scholar]
  18. Myers C. R., Nealson K. H. 1988; Bacterial manganese reduction and growth with manganese oxide as the sole electron acceptor. Science 240:1319–1321 [CrossRef]
     [Google Scholar]
  19. Nealson K. H., Myers C. R., Wimpee B. 1991; Isolation and identification of manganese-reducing bacteria, and estimates of microbial Mn(IV)-reducing potential in the Black Sea. Deep Sea Res 38: Suppl 2S907–S920 [CrossRef]
     [Google Scholar]
  20. Nogi Y., Kato C., Horikoshi K. 1998; Taxonomic studies of deep-sea barophilic Shewanella strains and description of Shewanella violacea sp. nov. Arch Microbiol 170:331–338 [CrossRef]
     [Google Scholar]
  21. Nozue H., Hayashi T., Hashimoto Y., Ezaki T., Hamasaki K., Ohwada K., Terawaki Y. 1992; Isolation and characterization of Shewanella alga from human clinical specimens and emendation of the description of S. alga Simidu et al 1990, 335). Int J Syst Bacteriol 42:628–634 [CrossRef]
     [Google Scholar]
  22. Sasser M. 1990; Identification of bacteria by gas chromatography of cellular fatty acids. Technical Note 101 Newark, DE: MIDI;
     [Google Scholar]
  23. Satomi M., Oikawa H., Yano Y. 2003; Shewanella marinintestina sp. nov., Shewanella schlegeliana sp. nov. and Shewanella sairae sp. nov., novel eicosapentaenoic-acid-producing marine bacteria isolated from sea-animal intestines. Int J Syst Evol Microbiol 53:491–499 [CrossRef]
     [Google Scholar]
  24. Semple K. M., Westlake D. W. S. 1987; Characterization of iron-reducing Alteromonas putrefaciens strains from oil field fluids. Can J Microbiol 33:366–371 [CrossRef]
     [Google Scholar]
  25. Stackebrandt E., Goebel B. M. 1994; Taxonomic note: a place for DNA-DNA reassociation and 16S rRNA sequence analysis in the present species definition in bacteriology. Int J Syst Bacteriol 44:846–849 [CrossRef]
     [Google Scholar]
  26. Tamaoka J., Komagata K. 1984; Determination of DNA base composition by reverse-phase high-performance liquid chromatography. FEMS Microbiol Lett 25:125–128 [CrossRef]
     [Google Scholar]
  27. Venkateswaran K., Moser D. P., Dollhopf M. E. 10 other authors 1999; Polyphasic taxonomy of the genus Shewanella and description of Shewanella oneidensis sp. nov. Int J Syst Bacteriol 49:705–724 [CrossRef]
     [Google Scholar]
  28. Wayne L. G., Brenner D. J., Colwell R. R. 9 other authors 1987; International Committee on Systematic Bacteriology. Report of the ad hoc committee on reconciliation of approaches to bacterial systematics. Int J Syst Bacteriol 37:463–464 [CrossRef]
     [Google Scholar]
  29. Weiner R. M., Coyne V. E., Brayton P., West P., Raiken S. F. 1988; Alteromonas colwelliana sp. nov., an isolate from oyster habitats. Int J Syst Bacteriol 38:240–244 [CrossRef]
     [Google Scholar]
  30. Yoon J.-H., Kim H., Kim S.-B., Kim H.-J., Kim W. Y., Lee S. T., Goodfellow M., Park Y.-H. 1996; Identification of Saccharomonospora strains by the use of genomic DNA fragments and rRNA gene probes. Int J Syst Bacteriol 46:502–505 [CrossRef]
     [Google Scholar]
  31. Yoon J.-H., Lee S. T., Park Y.-H. 1998; Inter- and intraspecific phylogenetic analysis of the genus Nocardioides and related taxa based on 16S rDNA sequences. Int J Syst Bacteriol 48:187–194 [CrossRef]
     [Google Scholar]
  32. Yoon J.-H., Kim I.-G., Shin D.-Y., Kang K. H., Park Y.-H. 2003; Microbulbifer salipaludis sp. nov., a moderate halophile isolated from a Korean salt marsh. Int J Syst Evol Microbiol 53:53–57 [CrossRef]
     [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/ijsem/10.1099/ijs.0.63198-0
Loading
Shewanella marisflavi sp. nov. and Shewanella aquimarina sp. nov., slightly halophilic organisms isolated from sea water of the Yellow Sea in Korea
Int J Syst Evol Microbiol 54, 2347 (2004); https://doi.org/10.1099/ijs.0.63198-0
/content/journal/ijsem/10.1099/ijs.0.63198-0
/content/journal/ijsem/10.1099/ijs.0.63198-0
Loading

Data & Media loading...

Supplements

Supplementary material 1

PDF

Most cited Most Cited RSS feed

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error