1887

Abstract

Two Gram-negative, motile, non-spore-forming, rod-shaped organisms, strains SW-117 and SW-120, were isolated from sea water of the Yellow Sea in Korea and subjected to a polyphasic taxonomic study. Strains SW-117 and SW-120 simultaneously contained both menaquinones (MK) and ubiquinones (Q) as isoprenoid quinones; the predominant menaquinone was MK-7 and the predominant ubiquinones were Q-7 and Q-8. The major fatty acid detected in the two strains was iso-C. The DNA G+C content of strains SW-117 and SW-120 was 51 and 54 mol%, respectively. Phylogenetic analyses based on 16S rRNA gene sequences showed that strains SW-117 and SW-120 fall within the radiation of the cluster comprising species. Strains SW-117 and SW-120 showed a 16S rRNA gene sequence similarity of 97·4 % and a DNA–DNA relatedness level of 10·1 %. Strains SW-117 and SW-120 exhibited 16S rRNA gene sequence similarity levels of 93·8–98·5 % and 92·4–97·0 %, respectively, to species. Strain SW-117 exhibited DNA–DNA relatedness levels of 8·3–20·3 % to the type strains of six phylogenetically related species. On the basis of phenotypic, phylogenetic and genetic data, strains SW-117 and SW-120 were classified in the genus as two distinct novel species, for which the names sp. nov. (type strain, SW-117=KCCM 41822=JCM 12192) and sp. nov. (type strain, SW-120=KCCM 41821=JCM 12193) are proposed, respectively.

Loading

Article metrics loading...

/content/journal/ijsem/10.1099/ijs.0.63198-0
2004-11-01
2020-01-28
Loading full text...

Full text loading...

/deliver/fulltext/ijsem/54/6/ijs542347.html?itemId=/content/journal/ijsem/10.1099/ijs.0.63198-0&mimeType=html&fmt=ahah

References

  1. Anzai, Y., Kim, H., Park, J.-Y., Wakabayashi, H. & Oyaizu, H. ( 2000; ). Phylogenetic affiliation of the pseudomonads based on 16S rRNA sequence. Int J Syst Evol Microbiol 50, 1563–1589.[CrossRef]
    [Google Scholar]
  2. Baumann, L. & Baumann, P. ( 1981; ). The marine Gram-negative eubacteria; genera Photobacterium, Beneckea, Alteromonas, Pseudomonas, and Alcaligenes. In The Prokaryotes. A Handbook on Habitats, Isolation, and Identification of Bacteria, pp. 1302–1330. Edited by M. P. Starr, H. Stolp, H. G. Trüper, A. Balows & H. G. Schlegel. Berlin: Springer.
  3. Bowman, J. P., McCammon, S. A., Nichols, D. S., Skerratt, J. H., Rea, S. M., Nichols, P. D. & McMeekin, T. A. ( 1997; ). Shewanella gelidimarina sp. nov. and Shewanella frigidimarina sp. nov., novel Antarctic species with the ability to produce eicosapentaenoic acid (20 : 5ω3) and grow anaerobically by dissimilatory Fe(III) reduction. Int J Syst Bacteriol 47, 1040–1047.[CrossRef]
    [Google Scholar]
  4. Bozal, N., Montes, M. J., Tudela, E., Jiménez, F. & Guinea, J. ( 2002; ). Shewanella frigidimarina and Shewanella livingstonensis sp. nov. isolated from Antarctic coastal areas. Int J Syst Evol Microbiol 52, 195–205.
    [Google Scholar]
  5. Brink, A. J., van Straten, A. & van Rensburg, A. J. ( 1995; ). Shewanella (Pseudomonas) putrefaciens bacteremia. Clin Infect Dis 20, 1327–1332.[CrossRef]
    [Google Scholar]
  6. Bruns, A., Rohde, M. & Berthe-Corti, L. ( 2001; ). Muricauda ruestringensis gen. nov., sp. nov., a facultatively anaerobic, appendaged bacterium from German North Sea intertidal sediment. Int J Syst Evol Microbiol 51, 1997–2006.[CrossRef]
    [Google Scholar]
  7. Cowan, S. T. & Steel, K. J. ( 1965; ). Manual for the Identification of Medical Bacteria. London: Cambridge University Press.
  8. Ezaki, T., Hashimoto, Y. & Yabuuchi, E. ( 1989; ). Fluorometric deoxyribonucleic acid-deoxyribonucleic acid hybridization in microdilution wells as an alternative to membrane filter hybridization in which radioisotopes are used to determine genetic relatedness among bacterial strains. Int J Syst Bacteriol 39, 224–229.[CrossRef]
    [Google Scholar]
  9. Ivanova, E. P., Sawabe, T., Gorshkova, N. M., Svetashev, V. I., Mikhailov, V. V., Nicolau, D. V. & Christen, R. ( 2001; ). Shewanella japonica sp. nov. Int J Syst Evol Microbiol 51, 1027–1033.[CrossRef]
    [Google Scholar]
  10. Ivanova, E. P., Sawabe, T., Hayashi, K., Gorshkova, N. M., Zhukova, N. V., Nedashkovskaya, O. I., Mikhailov, V. V., Nicolau, D. V. & Christen, R. ( 2003a; ). Shewanella fidelis sp. nov., isolated from sediments and sea water. Int J Syst Evol Microbiol 53, 577–582.[CrossRef]
    [Google Scholar]
  11. Ivanova, E. P., Nedashkovskaya, O. I., Zhukova, N. V., Nicolau, D. V., Christen, R. & Mikhailov, V. V. ( 2003b; ). Shewanella waksmanii sp. nov., isolated from a sipuncula (Phascolosoma japonicum). Int J Syst Evol Microbiol 53, 1471–1477.[CrossRef]
    [Google Scholar]
  12. Ivanova, E. P., Nedashkovskaya, O. I., Sawabe, T., Zhukova, N. V., Frolova, G. M., Nicolau, D. V., Mikhailov, V. V. & Bowman, J. P. ( 2004; ). Shewanella affinis sp. nov., isolated from marine invertebrates. Int J Syst Evol Microbiol 54, 1089–1093.[CrossRef]
    [Google Scholar]
  13. Komagata, K. & Suzuki, K.-I. ( 1987; ). Lipids and cell-wall analysis in bacterial systematics. Methods Microbiol 19, 161–203.
    [Google Scholar]
  14. Lanyi, B. ( 1987; ). Classical and rapid identification methods for medically important bacteria. Methods Microbiol 19, 1–67.
    [Google Scholar]
  15. Leifson, E. ( 1963; ). Determination of carbohydrate metabolism of marine bacteria. J Bacteriol 85, 1183–1184.
    [Google Scholar]
  16. Levring, T. ( 1946; ). Some culture experiments with Ulva and artificial seawater. K Fysiogr Sällsk Lund Förh 16, 45–56.
    [Google Scholar]
  17. MacDonell, M. T. & Colwell, R. R. ( 1985; ). Phylogeny of the Vibrionaceae, and recommendation for two new genera, Listonella and Shewanella. Syst Appl Microbiol 6, 171–182.[CrossRef]
    [Google Scholar]
  18. Myers, C. R. & Nealson, K. H. ( 1988; ). Bacterial manganese reduction and growth with manganese oxide as the sole electron acceptor. Science 240, 1319–1321.[CrossRef]
    [Google Scholar]
  19. Nealson, K. H., Myers, C. R. & Wimpee, B. ( 1991; ). Isolation and identification of manganese-reducing bacteria, and estimates of microbial Mn(IV)-reducing potential in the Black Sea. Deep Sea Res 38, Suppl 2, S907–S920.[CrossRef]
    [Google Scholar]
  20. Nogi, Y., Kato, C. & Horikoshi, K. ( 1998; ). Taxonomic studies of deep-sea barophilic Shewanella strains and description of Shewanella violacea sp. nov. Arch Microbiol 170, 331–338.[CrossRef]
    [Google Scholar]
  21. Nozue, H., Hayashi, T., Hashimoto, Y., Ezaki, T., Hamasaki, K., Ohwada, K. & Terawaki, Y. ( 1992; ). Isolation and characterization of Shewanella alga from human clinical specimens and emendation of the description of S. alga Simidu et al. 1990, 335. Int J Syst Bacteriol 42, 628–634.[CrossRef]
    [Google Scholar]
  22. Sasser, M. ( 1990; ). Identification of bacteria by gas chromatography of cellular fatty acids. Technical Note 101. Newark, DE: MIDI.
  23. Satomi, M., Oikawa, H. & Yano, Y. ( 2003; ). Shewanella marinintestina sp. nov., Shewanella schlegeliana sp. nov. and Shewanella sairae sp. nov., novel eicosapentaenoic-acid-producing marine bacteria isolated from sea-animal intestines. Int J Syst Evol Microbiol 53, 491–499.[CrossRef]
    [Google Scholar]
  24. Semple, K. M. & Westlake, D. W. S. ( 1987; ). Characterization of iron-reducing Alteromonas putrefaciens strains from oil field fluids. Can J Microbiol 33, 366–371.[CrossRef]
    [Google Scholar]
  25. Stackebrandt, E. & Goebel, B. M. ( 1994; ). Taxonomic note: a place for DNA-DNA reassociation and 16S rRNA sequence analysis in the present species definition in bacteriology. Int J Syst Bacteriol 44, 846–849.[CrossRef]
    [Google Scholar]
  26. Tamaoka, J. & Komagata, K. ( 1984; ). Determination of DNA base composition by reverse-phase high-performance liquid chromatography. FEMS Microbiol Lett 25, 125–128.[CrossRef]
    [Google Scholar]
  27. Venkateswaran, K., Moser, D. P., Dollhopf, M. E. & 10 other authors ( 1999; ). Polyphasic taxonomy of the genus Shewanella and description of Shewanella oneidensis sp. nov. Int J Syst Bacteriol 49, 705–724.[CrossRef]
    [Google Scholar]
  28. Wayne, L. G., Brenner, D. J., Colwell, R. R. & 9 other authors ( 1987; ). International Committee on Systematic Bacteriology. Report of the ad hoc committee on reconciliation of approaches to bacterial systematics. Int J Syst Bacteriol 37, 463–464.[CrossRef]
    [Google Scholar]
  29. Weiner, R. M., Coyne, V. E., Brayton, P., West, P. & Raiken, S. F. ( 1988; ). Alteromonas colwelliana sp. nov., an isolate from oyster habitats. Int J Syst Bacteriol 38, 240–244.[CrossRef]
    [Google Scholar]
  30. Yoon, J.-H., Kim, H., Kim, S.-B., Kim, H.-J., Kim, W. Y., Lee, S. T., Goodfellow, M. & Park, Y.-H. ( 1996; ). Identification of Saccharomonospora strains by the use of genomic DNA fragments and rRNA gene probes. Int J Syst Bacteriol 46, 502–505.[CrossRef]
    [Google Scholar]
  31. Yoon, J.-H., Lee, S. T. & Park, Y.-H. ( 1998; ). Inter- and intraspecific phylogenetic analysis of the genus Nocardioides and related taxa based on 16S rDNA sequences. Int J Syst Bacteriol 48, 187–194.[CrossRef]
    [Google Scholar]
  32. Yoon, J.-H., Kim, I.-G., Shin, D.-Y., Kang, K. H. & Park, Y.-H. ( 2003; ). Microbulbifer salipaludis sp. nov., a moderate halophile isolated from a Korean salt marsh. Int J Syst Evol Microbiol 53, 53–57.[CrossRef]
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/ijsem/10.1099/ijs.0.63198-0
Loading
/content/journal/ijsem/10.1099/ijs.0.63198-0
Loading

Data & Media loading...

Supplements

vol. , part 6, pp. 2347–2352

Detailed phenotypic characteristics of , and related species, and an expanded neighbour-joining tree are available to download. [PDF](89KB)



PDF

Most cited articles

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error