1887

Abstract

Eight strains of Gram-negative bacteria able to form ring-like cells were isolated from Mahoney Lake, a meromictic lake in south-central British Columbia, Canada. All strains were pink–purple and contained bacteriochlorophyll incorporated into the light-harvesting 1 and 2 and reaction-centre pigment–protein complexes. Growth did not occur anaerobically under illuminated conditions; these strains were obligately aerobic, prompting their designation as members of the aerobic phototrophic bacteria. Physiological characterization revealed that these isolates share a similar tolerance to high levels of salinity and pH, as would be expected of bacteria from a highly saline lake; however, the strains exhibited marked differences in their ability to utilize organic substrates for aerobic heterotrophic growth. 16S rRNA sequence analysis showed that the strains are closely related to members of the non-phototrophic genera (92·0–92·9 %) and (92·2–92·6 %), as well as to aerobic phototrophs of the genera (92·2–92·9 %) and (91·7–92·4 %) within the ‘’. The DNA G+C content was 66·2 mol%. The unusual light-harvesting complex 2, the distinct morphological features and physiological traits of these strains as well as the phylogenetic data support the proposal of the novel genus and species gen. nov., sp. nov., with ML6 (=DSM 16097=VKM B-2346) as the type strain.

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2005-07-01
2019-10-20
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References

  1. De Soete, G. ( 1983; ). A least squares algorithm for fitting additive trees to proximity data. Psychometrika 48, 621–626.[CrossRef]
    [Google Scholar]
  2. Gosink, J. J., Herwig, R. P. & Staley, J. T. ( 1997; ). Octadecabacter arcticus gen. nov., sp. nov., and O. antarcticus, sp. nov., nonpigmented, psychrophilic gas vacuolated bacteria from polar sea ice and water. Syst Appl Microbiol 20, 356–365.[CrossRef]
    [Google Scholar]
  3. Hall, K. J. & Northcote, T. G. ( 1986; ). Conductivity-temperature standardization and dissolved solids estimation in a meromictic saline lake. Can J Fish Aquat Sci 43, 2450–2454.[CrossRef]
    [Google Scholar]
  4. Imhoff, J. F. ( 1988; ). Anoxygenic phototrophic bacteria. In Methods in Aquatic Bacteriology, pp. 207–240. Edited by B. Austin. New York: Wiley.
  5. Imhoff, J. F. ( 2001; ). The phototrophic alpha-Proteobacteria. In The Prokaryotes: an Evolving Electronic Resource for the Microbiological Community, 3rd edn, release 3.6, 22 June 2001. Edited by M. Dworkin et al. New York: Springer. http://link.springer-ny.com/link/service/books/10125/
  6. Kellenberger, E., Ryter, A. & Sechaud, J. ( 1958; ). Electron microscope study of DNA-containing plasms. II. Vegetative and mature phage DNA as compared with normal bacterial nucleoids in different physiological states. J Biophys Biochem Cytol 4, 671–678.[CrossRef]
    [Google Scholar]
  7. Labrenz, M., Collins, M. D., Lawson, P. A., Tindall, B. J., Schumann, P. & Hirsch, P. ( 1999; ). Roseovarius tolerans gen. nov., sp. nov., a budding bacterium with variable bacteriochlorophyll a production from hypersaline Ekho Lake. Int J Syst Bacteriol 49, 137–147.[CrossRef]
    [Google Scholar]
  8. Lybarger, S. R. & Maddock, J. R. ( 2001; ). Polarity in action: asymmetric protein localization in bacteria. J Bacteriol 183, 3261–3267.[CrossRef]
    [Google Scholar]
  9. Maidak, B. L., Olsen, G. J., Larsen, N., Overbeek, R., McCaughey, M. J. & Woese, C. R. ( 1996; ). The Ribosomal Database Project (RDP). Nucleic Acids Res 24, 82–85.[CrossRef]
    [Google Scholar]
  10. Mesbah, M. & Whitman, W. B. ( 1989; ). Measurement of deoxyguanosine/thymidine ratios in complex mixtures by high-performance liquid chromatography for determination of the mole percentage guanine + cytosine of DNA. J Chromatogr 479, 297–306.[CrossRef]
    [Google Scholar]
  11. Northcote, T. G. & Halsey, T. G. ( 1969; ). Seasonal changes in the limnology of some meromictic lakes in southern British Columbia. J Fish Res Board Can 26, 1763–1787.[CrossRef]
    [Google Scholar]
  12. Overman, J., Beatty, J. T., Hall, K. J., Pfennig, N. & Northcote, T. G. ( 1991; ). Characterization of a dense, purple sulfur bacterial layer in a meromictic salt lake. Limnol Oceanogr 36, 846–859.[CrossRef]
    [Google Scholar]
  13. Overman, J., Beatty, J. T. & Hall, K. J. ( 1994; ). Photosynthetic activity and population dynamics of Amoebobacter purpureus in a meromictic saline lake. FEMS Microbiol Ecol 15, 309–320.[CrossRef]
    [Google Scholar]
  14. Overman, J., Beatty, J. T. & Hall, K. J. ( 1996; ). Purple sulfur bacteria control the growth of aerobic heterotrophic bacterioplankton in a meromictic salt lake. Appl Environ Microbiol 62, 3251–3258.
    [Google Scholar]
  15. Pfennig, N. ( 1969; ). Rhodospirillum tenue sp. n., a new species of the purple nonsulfur bacteria. J Bacteriol 99, 619–620.
    [Google Scholar]
  16. Pfennig, N. ( 1978; ). Rhodocyclus purpureus gen. nov. and sp. nov., a ring-shaped vitamin B12-requiring member of the family Rhodospirillaceae. Int J Syst Bacteriol 28, 283–288.[CrossRef]
    [Google Scholar]
  17. Rainey, F. A., Ward-Rainey, N., Kroppenstedt, R. M. & Stackebrandt, E. ( 1996; ). The genus Nocardiopsis represents a phylogenetically coherent taxon and a distinct actinomycete lineage: proposal of Nocardiopsaceae fam. nov. Int J Syst Bacteriol 46, 1088–1092.[CrossRef]
    [Google Scholar]
  18. Rathgeber, C., Beatty, J. T. & Yurkov, V. ( 2004; ). Aerobic phototrophic bacteria: new evidence for the diversity, ecological importance and applied potential of this previously overlooked group. Photosynth Res 81, 113–128.[CrossRef]
    [Google Scholar]
  19. Shiba, T. ( 1991; ). Roseobacter litoralis gen. nov., sp. nov. and Roseobacter denitrificans sp. nov., aerobic pink-pigmented bacteria which contain bacteriochlorophyll a. Syst Appl Microbiol 14, 140–145.[CrossRef]
    [Google Scholar]
  20. Shiba, T. & Simidu, U. ( 1982; ). Erythrobacter longus gen. nov., sp. nov., an aerobic bacterium which contains bacteriochlorophyll a. Int J Syst Bacteriol 32, 211–217.[CrossRef]
    [Google Scholar]
  21. Shiba, T., Simidu, U. & Taga, N. ( 1979; ). Distribution of aerobic bacteria which contain bacteriochlorophyll a. Appl Environ Microbiol 38, 43–45.
    [Google Scholar]
  22. Suzuki, T., Muroga, Y., Takahama, M. & Nishimura, Y. ( 1999; ). Roseivivax halodurans gen. nov., sp. nov. and Roseivivax halotolerans sp. nov., aerobic bacteriochlorophyll-containing bacteria isolated from a saline lake. Int J Syst Bacteriol 49, 629–634.[CrossRef]
    [Google Scholar]
  23. Tamaoka, J. & Komagata, K. ( 1984; ). Determination of DNA base composition by reversed-phase high-performance liquid chromatography. FEMS Microbiol Lett 25, 125–128.[CrossRef]
    [Google Scholar]
  24. Urbance, J. W., Bratina, B. J., Stoddard, S. F. & Schmidt, T. M. ( 2001; ). Taxonomic characterization of Ketogulonigenium vulgare gen. nov., sp. nov., and Ketogulonigenium robustum sp. nov., which oxidize l-sorbose to 2-keto-l-gulonic acid. Int J Syst Evol Microbiol 51, 1059–1070.[CrossRef]
    [Google Scholar]
  25. Yurkov, V. V. & Beatty, J. T. ( 1998; ). Aerobic anoxygenic phototrophic bacteria. Microbiol Mol Biol Rev 62, 695–724.
    [Google Scholar]
  26. Yurkov, V. & van Gemerden, H. ( 1993; ). Abundance and salt tolerance of obligately aerobic, phototrophic bacteria in a microbial mat. Neth J Sea Res 31, 57–62.[CrossRef]
    [Google Scholar]
  27. Yurkov, V., Stackebrandt, E., Holmes, A. & 7 other authors ( 1994; ). Phylogenetic positions of novel aerobic, bacteriochlorophyll a-containing bacteria and description of Roseococcus thiosulfatophilus gen. nov., sp. nov., Erythromicrobium ramosum gen. nov., sp. nov., and Erythrobacter litoralis sp. nov. Int J Syst Bacteriol 44, 427–434.[CrossRef]
    [Google Scholar]
  28. Yurkov, V. V., Krieger, S., Stackebrandt, E. & Beatty, J. T. ( 1999; ). Citromicrobium bathyomarinum, a novel aerobic bacterium isolated from deep-sea hydrothermal vent plume waters that contains photosynthetic pigment-protein complexes. J Bacteriol 181, 4517–4525.
    [Google Scholar]
  29. Yurkova, N., Rathgeber, C., Swiderski, J., Stackebrandt, E., Beatty, J. T., Hall, K. J. & Yurkov, V. ( 2002; ). Diversity, distribution and physiology of the aerobic phototrophic bacteria in the mixolimnion of a meromictic lake. FEMS Microbiol Ecol 40, 191–204.[CrossRef]
    [Google Scholar]
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