1887

Abstract

A moderately halophilic bacterium, strain SS20, capable of growing at salinities of 1–20 % (w/v) NaCl was isolated from a solar saltern of the Dangjin area in Korea and was characterized taxonomically. Strain SS20 was a Gram-negative bacterium comprising motile, short rods. Its major cellular fatty acids were C 7, C 8 cyclo and C. The DNA G+C content was 70 mol% and the predominant ubiquinone was Q-9. Phylogenetic analysis based on 16S rRNA gene sequences showed that strain SS20 belonged to the genus . The levels of 16S rRNA gene sequence similarity to the type strains of species were in the range 93·0–97·5 %. The levels of DNA–DNA relatedness between strain SS20 and the type strains of phylogenetically closely related species were in the range 5·3–12·3 %. On the basis of physiological and molecular properties, strain SS20 represents a novel species of the genus , for which the name sp. nov. is proposed. The type strain is SS20 (=KCTC 12127=JCM 12237).

Loading

Article metrics loading...

/content/journal/ijsem/10.1099/ijs.0.63194-0
2004-11-01
2019-10-19
Loading full text...

Full text loading...

/deliver/fulltext/ijsem/54/6/ijs542037.html?itemId=/content/journal/ijsem/10.1099/ijs.0.63194-0&mimeType=html&fmt=ahah

References

  1. Arahal, D. R., García, M. T., Vargas, C., Cánovas, D., Nieto, J. J. & Ventosa, A. ( 2001; ). Chromohalobacter salexigens sp. nov., a moderately halophilic species that includes Halomonas elongata DSM 3043 and ATCC 33174. Int J Syst Evol Microbiol 51, 1457–1462.
    [Google Scholar]
  2. Arahal, D. R., Castillo, A. M., Ludwig, W., Schleifer, K. H. & Ventosa, A. ( 2002a; ). Proposal of Cobetia marina gen. nov., comb. nov., within the family Halomonadaceae, to include the species Halomonas marina. Syst Appl Microbiol 25, 207–211.[CrossRef]
    [Google Scholar]
  3. Arahal, D. R., Ludwig, W., Schleifer, K. H. & Ventosa, A. ( 2002b; ). Phylogeny of the family Halomonadaceae based on 23S and 16S rDNA sequence analyses. Int J Syst Evol Microbiol 52, 241–249.
    [Google Scholar]
  4. Baumann, L., Bowditch, R. D. & Baumann, P. ( 1983; ). Description of Deleya gen. nov. created to accommodate the marine species Alcaligenes aestus, A. pacificus, A. cupidus, A. venustus, and Pseudomonas marina. Int J Syst Bacteriol 33, 793–802.[CrossRef]
    [Google Scholar]
  5. Bouchotroch, S., Quesada, E., Moral, A. D., Llamas, I. & Béjar, V. ( 2001; ). Halomonas maura sp. nov., a novel moderately halophilic, exopolysaccharide-producing bacterium. Int J Syst Evol Microbiol 51, 1625–1632.[CrossRef]
    [Google Scholar]
  6. Cowan, S. T. & Steel, K. J. ( 1965; ). Manual for the Identification of Medical Bacteria. London: Cambridge University Press.
  7. DeLong, E. F. ( 1992; ). Archaea in coastal marine environments. Proc Natl Acad Sci U S A 89, 5685–5689.[CrossRef]
    [Google Scholar]
  8. Dobson, S. J. & Franzmann, P. D. ( 1996; ). Unification of the genera Deleya ( Baumann et al. 1983 ), Halomonas ( Vreeland et al. 1980 ), and Halovibrio (Fendrich 1988) and the species Paracoccus halodenitrificans (Robinson and Gibbons 1952) into a single genus, Halomonas, and placement of the genus Zymobacter in the family Halomonadaceae. Int J Syst Bacteriol 46, 550–558.[CrossRef]
    [Google Scholar]
  9. Ezaki, T., Hashimoto, Y. & Yabuuchi, E. ( 1989; ). Fluorometric deoxyribonucleic acid-deoxyribonucleic acid hybridization in microdilution wells as an alternative to membrane filter hybridization in which radioisotopes are used to determine genetic relatedness among bacterial strains. Int J Syst Bacteriol 39, 224–229.[CrossRef]
    [Google Scholar]
  10. Felsenstein, J. ( 1981; ). Evolutionary trees from DNA sequences: a maximum likelihood approach. J Mol Bacteriol 39, 224–229.
    [Google Scholar]
  11. Felsenstein, J. ( 2002; ). phylip (phylogeny inference package), version 3.6a. Distributed by the author. Department of Genetics, University of Washington, Seattle, USA.
  12. Franzmann, P. D. & Tindall, B. J. ( 1990; ). A chemotaxonomic study of members of the family Halomonadaceae. Syst Appl Microbiol 13, 142–147.[CrossRef]
    [Google Scholar]
  13. García, M. T., Mellado, E., Ostos, J. C. & Ventosa, A. ( 2004; ). Halomonas organivorans sp. nov., a moderate halophile able to degrade aromatic compounds. Int J Syst Evol Microbiol 54, 1723–1728.[CrossRef]
    [Google Scholar]
  14. Kaye, J. Z., Márquez, M. C., Ventosa, A. & Baross, J. A. ( 2004; ). Halomonas neptunia sp. nov., Halomonas sulfidaeris sp. nov., Halomonas axialensis sp. nov. and Halomonas hydrothermalis sp. nov.: halophilic bacteria isolated from deep-sea hydrothermal-vent environments. Int J Syst Evol Microbiol 54, 499–511.[CrossRef]
    [Google Scholar]
  15. Kimura, M. ( 1980; ). A simple method for estimating evolutionary rates of base substitutions through comparative studies of nucleotide sequences. J Mol Evol 16, 111–120.[CrossRef]
    [Google Scholar]
  16. Komagata, K. & Suzuki, K. ( 1987; ). Lipid and cell-wall analysis in bacterial systematics. Methods Microbiol 19, 161–208.
    [Google Scholar]
  17. Lanyi, B. ( 1987; ). Classical and rapid identification methods for medically important bacteria. Methods Microbiol 19, 1–67.
    [Google Scholar]
  18. Margesin, R. & Schinner, F. ( 2001; ). Potential of halotolerant and halophilic microorganisms for biotechnology. Extremophiles 5, 73–83.[CrossRef]
    [Google Scholar]
  19. Mata, J. A., Martínez-Cánovas, M. J., Quesada, E. & Béjar, V. ( 2002; ). A detailed phenotypic characterization of the type strains of Halomonas species. Syst Appl Microbiol 25, 360–375.[CrossRef]
    [Google Scholar]
  20. Mellado, E., Moore, E. R. B., Nieto, J. J. & Ventosa, A. ( 1995; ). Phylogenetic inferences and taxonomic consequences of 16S ribosomal DNA sequence comparison of Chromohalobacter marismortui, Volcaniella eurihalina, and Deleya salina and reclassification of V. eurihalina as Halomonas eurihalina comb. nov. Int J Syst Bacteriol 45, 712–716.[CrossRef]
    [Google Scholar]
  21. Okamoto, T., Taguchi, H., Nakamura, K., Ikenaga, H., Kuraishi, H. & Yamasato, K. ( 1993; ). Zymobacter palmae gen. nov., sp. nov., a new ethanol-fermenting peritrichous bacterium isolated from palm sap. Arch Microbiol 160, 333–337.
    [Google Scholar]
  22. Quesada, E., Valderrama, M. J., Bejar, V., Ventosa, A., Gutiérrez, M. C., Ruiz-Berraquero, F. & Ramos-Cormenzana, A. ( 1990; ). Volcaniella eurihalina gen. nov., sp. nov., a moderately halophilic nonmotile gram-negative rod. Int J Syst Bacteriol 40, 261–267.[CrossRef]
    [Google Scholar]
  23. Reddy, G. S. N., Raghavan, P. U. M., Sarita, N. B., Prakash, J. S. S., Nagesh, N., Delille, D. & Shivaji, S. ( 2003; ). Halomonas glaciei sp. nov. isolated from fast ice of Adelie Land, Antarctica. Extremophiles 7, 55–61.
    [Google Scholar]
  24. Romanenko, L. A., Schumann, P., Roche, M., Mikhailov, V. V. & Stackebrandt, E. ( 2002; ). Halomonas halocynthiae sp. nov., isolated from the marine ascidian Halocynthia aurantium. Int J Syst Evol Microbiol 52, 1767–1772.[CrossRef]
    [Google Scholar]
  25. Saitou, N. & Nei, M. ( 1987; ). The neighbor-joining method: a new method for reconstructing phylogenetic trees. Mol Biol Evol 4, 406–425.
    [Google Scholar]
  26. Thompson, J. D., Higgins, D. G. & Gibson, T. J. ( 1994; ). clustal w: improving the sensitivity of progressive multiple sequence alignment through sequence weighting, position-specific gap penalties and weight matrix choice. Nucleic Acids Res 22, 4673–4680.[CrossRef]
    [Google Scholar]
  27. Tamaoka, J. & Komagata, K. ( 1984; ). Determination of DNA base composition by reverse-phase high-performance liquid chromatography. FEMS Microbiol Lett 25, 125–128.[CrossRef]
    [Google Scholar]
  28. Valderrama, M. J., Quesada, E., Bejar, V., Ventosa, A., Gutierrez, M. C., Berraquero, F. R. & Cormenzana, A. R. ( 1991; ). Deleya salina sp. nov., a moderately halophilic Gram-negative bacterium. Int J Syst Bacteriol 41, 377–384.[CrossRef]
    [Google Scholar]
  29. Valderrama, M. J., Monteoliva-Sanchez, M., Quesada, E. & Ramos-Cormenzana, A. ( 1998; ). Influence of salt concentration on the cellular fatty acid composition of the moderately halophilic bacterium Halomonas salina. Res Microbiol 149, 675–679.[CrossRef]
    [Google Scholar]
  30. Ventosa, A., Nieto, J. J. & Oren, A. ( 1998; ). Biology of moderately halophilic aerobic bacteria. Microbiol Mol Biol Rev 62, 504–544.
    [Google Scholar]
  31. Vreeland, R. H., Litchfield, C. D., Martin, E. L. & Elliot, E. ( 1980; ). Halomonas elongata, a new genus and species of extremely salt-tolerant bacteria. Int J Syst Bacteriol 30, 485–495.[CrossRef]
    [Google Scholar]
  32. Wayne, L. G. ( 1994; ). Actions of the Judicial Commission of the International Committee on Systematic Bacteriology on requests for opinions published between January 1985 and July 1993. Int J Syst Bacteriol 44, 177–178.[CrossRef]
    [Google Scholar]
  33. Yakimov, M. M., Golyshin, P. N., Lang, S., Moore, E. R., Abraham, W. R., Lunsdorf, H. & Timmis, K. N. ( 1998; ). Alcanivorax borkumensis gen. nov., sp. nov., a new, hydrocarbon-degrading and surfactant-producing marine bacterium. Int J Syst Bacteriol 48, 339–348.[CrossRef]
    [Google Scholar]
  34. Yoon, J.-H., Kim, H., Kim, S.-B., Kim, H.-J., Kim, W. Y., Lee, S. T., Goodfellow, M. & Park, Y.-H. ( 1996; ). Identification of Saccharomonospora strains by the use of genomic DNA fragments and rRNA gene probes. Int J Syst Bacteriol 46, 502–505.[CrossRef]
    [Google Scholar]
  35. Yoon, J.-H., Choi, S. H., Lee, K.-C., Kho, Y. H., Kang, K. H. & Park, Y.-H. ( 2001; ). Halomonas marisflavae sp. nov., a halophilic bacterium isolated from the Yellow Sea in Korea. Int J Syst Evol Microbiol 51, 1171–1177.[CrossRef]
    [Google Scholar]
  36. Yoon, J.-H., Lee, K.-C., Kho, Y.-H., Kang, K.-H., Kim, C.-J. & Park, Y.-H. ( 2002; ). Halomonas alimentaria sp. nov., isolated from jeotgal, a traditional Korean fermented seafood. Int J Syst Evol Microbiol 52, 123–130.
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/ijsem/10.1099/ijs.0.63194-0
Loading
/content/journal/ijsem/10.1099/ijs.0.63194-0
Loading

Data & Media loading...

Most Cited This Month

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error