Four isolates, which were obtained from Belgian, Moroccan and Romanian dairy products, constituted a homogeneous but unidentified taxon after screening with whole-cell protein fingerprinting. Complete 16S rRNA gene sequence analysis classified representative strains in the genus . Highest sequence similarities of 98·6 and 98·0 % were obtained with the species and , respectively. Growth characteristics, biochemical features, tRNA intergenic length polymorphism analysis, DNA–DNA hybridization and DNA G+C contents of selected strains demonstrated that they represent a single, novel species. It differs phenotypically from other enterococci in characteristics commonly considered as typical of this genus: no growth in 6·5 % NaCl or 0·4 % sodium azide, and no acid production from a wide range of carbohydrates. The name sp. nov. is proposed for this novel species; the type strain (LMG 21727=CCM 7220) was isolated from contaminated pasteurized cow's milk.


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vol. , part 6, pp. 2175 - 2179

Distance matrix tree showing the phylogenetic relationships of and other enterococcal reference species [PDF](140 KB)


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