1887

Abstract

Chemotaxonomic and morphological characterization of two actinomycete strains, MS1-3 and AS4-2, respectively isolated from moat sediment and scumming activated sludge, was carried out. This characterization clearly demonstrated that strains MS1-3 and AS4-2 belong to the genus . 16S rRNA gene sequencing studies showed that these isolates are most closely related to (98·1–98·3 % similarity), (97·9–98·0 %) and (97·8–97·9 %). However, the results of DNA–DNA hybridizations and physiological and biochemical tests showed that strains MS1-3 and AS4-2 could be differentiated from their closest phylogenetic relatives both genotypically and phenotypically. It is proposed that the two isolates be classified as representatives of a novel species of , sp. nov. The type strain is MS1-3 (=NBRC 100417=DSM 44801); AS4-2 (=NBRC 100418=DSM 44802) is a reference strain.

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2005-01-01
2020-01-23
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References

  1. Felsenstein, J. ( 1985; ). Confidence limits on phylogenies: an approach using the bootstrap. Evolution 39, 783–791.[CrossRef]
    [Google Scholar]
  2. Goodfellow, M. ( 1998; ). Nocardia and related genera. In Topley and Wilson's Microbiology and Microbial Infections, 9th edn, vol. 2, Systematic Bacteriology, pp. 463–489. Edited by A. Balows & B. I. Duerden. London: Arnold.
  3. Goodfellow, M. & Lechevalier, M. P. ( 1989; ). Genus Nocardia Trevisan 1889. In Bergey's Manual of Systematic Bacteriology, vol. 4, pp. 2350–2361. Edited by S. T. Williams, M. E. Sharpe & J. P. Holt. Baltimore: Williams & Wilkins.
  4. Goodfellow, M., Isik, K. & Yates, E. ( 1999; ). Actinomycete systematics: an unfinished synthesis. Nova Acta Leopold NF80 (312), 47–82.
    [Google Scholar]
  5. Gordon, R. E., Barnett, D. A., Handerhan, J. E. & Pang, C. H.-N. ( 1974; ). Nocardia coeliaca, Nocardia autotrophica, and the nocardin strain. Int J Syst Bacteriol 24, 54–63.[CrossRef]
    [Google Scholar]
  6. Hasegawa, T., Takizawa, M. & Tanida, S. ( 1983; ). A rapid analysis for chemical grouping of aerobic actinomycetes. J Gen Appl Microbiol 29, 319–322.[CrossRef]
    [Google Scholar]
  7. Hayakawa, M., Takeuchi, T. & Toyohiko, Y. ( 1996; ). Combined use of trimethoprim with nalidixic acid for the selective isolation and enumeration of actinomycetes from soil. Actinomycetologica 10, 80–90.[CrossRef]
    [Google Scholar]
  8. Isik, K., Chun, J., Hah, Y. C. & Goodfellow, M. ( 1999; ). Nocardia salmonicida nom. rev., a fish pathogen. Int J Syst Bacteriol 49, 833–837.[CrossRef]
    [Google Scholar]
  9. Kageyama, A., Yazawa, K., Nishimura, K. & Mikami, Y. ( 2004; ). Nocardia inohanensis sp. nov., Nocardia yamanashiensis sp. nov. and Nocardia niigatensis sp. nov., isolated from clinical specimens. Int J Syst Evol Microbiol 54, 563–569.[CrossRef]
    [Google Scholar]
  10. Kämpfer, P., Buczolits, S., Jäckel, U., Grün-Wollny, I. & Busse, H.-J. ( 2004; ). Nocardia tenerifensis sp. nov. Int J Syst Evol Microbiol 54, 381–383.[CrossRef]
    [Google Scholar]
  11. Kusunoki, S., Ezaki, T., Tamesada, S., Hatanaka, Y., Asano, K., Hashimoto, Y. & Yabuuchi, E. ( 1991; ). Application of colorimetric microdilution plate hybridization for rapid genetic identification of 22 Mycobacterium species. J Clin Microbiol 29, 1596–1603.
    [Google Scholar]
  12. Lechevalier, M. P. & Lechevalier, H. ( 1970; ). Chemical composition as a criterion in the classification of aerobic actinomycetes. Int J Syst Bacteriol 20, 435–443.[CrossRef]
    [Google Scholar]
  13. Lechevalier, M. P., De Bièvre, C. & Lechevalier, H. A. ( 1977; ). Chemotaxonomy of aerobic actinomycetes: phospholipid composition. Biochem Syst Ecol 5, 249–260.[CrossRef]
    [Google Scholar]
  14. Minnikin, D. E., O'Donnell, A. G., Goodfellow, M., Alderson, G., Athalye, M., Schaal, K. & Parlett, J. H. ( 1984; ). An integrated procedure for the extraction of bacterial isoprenoid quinones and polar lipids. J Microbiol Methods 2, 233–241.[CrossRef]
    [Google Scholar]
  15. Saintpierre-Bonaccio, D., Maldonado, L. A., Amir, H., Pineau, R. & Goodfellow, M. ( 2004; ). Nocardia neocaledoniensis sp. nov., a novel actinomycete isolated from New-Caledonian brown hypermagnesian ultramafic soil. Int J Syst Evol Microbiol 54, 599–603.[CrossRef]
    [Google Scholar]
  16. Saitou, N. & Nei, M. ( 1987; ). The neighbor-joining method: a new method for reconstructing phylogenetic trees. Mol Biol Evol 4, 406–425.
    [Google Scholar]
  17. Schaal, K. P. ( 1985; ). Identification of clinically significant actinomycetes and related bacteria using chemical techniques. In Chemical Methods Bacterial Systematics, pp. 359–381. Edited by M. Goodfellow & D. E. Minnikin. London: Academic Press.
  18. Stackebrandt, E., Rainey, F. A. & Ward-Rainey, N. L. ( 1997; ). Proposal for a new hierarchic classification system, Actinobacteria classis nov. Int J Syst Bacteriol 47, 479–491.[CrossRef]
    [Google Scholar]
  19. Tamura, T., Nakagaito, Y., Nishii, T., Hasegawa, T., Stackebrandt, E. & Yokota, A. ( 1994; ). A new genus of the order Actinomycetales, Couchioplanes gen. nov., with descriptions of Couchioplanes caeruleus (Horan and Brodsky 1986) comb. nov. and Couchioplanes caeruleus subsp. azureus subsp. nov. Int J Syst Bacteriol 44, 193–203.[CrossRef]
    [Google Scholar]
  20. Thompson, J. D., Higgins, D. G. & Gibson, T. J. ( 1994; ). clustal w: improving the sensitivity of progressive multiple sequence alignment through sequence weighting, position-specific gap penalties and weight matrix choice. Nucleic Acids Res 22, 4673–4680.[CrossRef]
    [Google Scholar]
  21. Torres, R. D., Oletta, C. A. & Zlotnik, H. ( 1996; ). A rapid and gentle method for isolation of genomic DNA from pathogenic Nocardia spp. Clin Diagn Lab Immunol 3, 601–604.
    [Google Scholar]
  22. Wang, L., Zhang, Y., Lu, Z., Shi, Y., Liu, Z., Maldonado, L. & Goodfellow, M. ( 2001; ). Nocardia beijingensis sp. nov., a novel isolate from soil. Int J Syst Evol Microbiol 51, 1783–1788.[CrossRef]
    [Google Scholar]
  23. Wayne, L. G., Brenner, D. J., Colwell, R. R. & 9 other authors ( 1987; ). International Committee on Systematic Bacteriology. Report of the ad hoc committee on reconciliation of approaches to bacterial systematics. Int J Syst Bacteriol 37, 463–464.[CrossRef]
    [Google Scholar]
  24. Yamamura, H., Hayakawa, M. & Iimura, Y. ( 2003a; ). Application of sucrose-gradient centrifugation for selective isolation of Nocardia spp. from soil. J Appl Microbiol 95, 677–685.[CrossRef]
    [Google Scholar]
  25. Yamamura, H., Hayakawa, M., Nakagawa, Y. & Iimura, Y. ( 2003b; ). Species diversity of nocardiae isolated from lake and moat sediment samples. Actinomycetologica 17, 44–46.[CrossRef]
    [Google Scholar]
  26. Yamamura, H., Hayakawa, M., Nakagawa, Y. & Iimura, Y. <1?show=[to]>( 2004; ). Characterization of Nocardia asteroides isolates from different ecological habitats on the basis of repetitive extragenic palindromic-PCR fingerprinting. Appl Environ Microbiol 70, 3149–3151.[CrossRef]
    [Google Scholar]
  27. Yassin, A. F., Sträubler, B., Schumann, P. & Schaal, K. P. ( 2003; ). Nocardia puris sp. nov. Int J Syst Evol Microbiol 53, 1595–1599.[CrossRef]
    [Google Scholar]
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Scanning electron micrographs of strain MS1-3 grown on oatmeal-YGG agar at 28 °C for 4 weeks.

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Phylogenetic tree derived from 16S rRNA gene sequences showing the relationship of strains MS1-3 and AS4-2 to all known species. The tree was constructed using the neighbour-joining method and values. Only values above 50 % significance are indicated. [PDF](18 KB)

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