1887

Abstract

Chemotaxonomic and morphological characterization of two actinomycete strains, MS1-3 and AS4-2, respectively isolated from moat sediment and scumming activated sludge, was carried out. This characterization clearly demonstrated that strains MS1-3 and AS4-2 belong to the genus . 16S rRNA gene sequencing studies showed that these isolates are most closely related to (98·1–98·3 % similarity), (97·9–98·0 %) and (97·8–97·9 %). However, the results of DNA–DNA hybridizations and physiological and biochemical tests showed that strains MS1-3 and AS4-2 could be differentiated from their closest phylogenetic relatives both genotypically and phenotypically. It is proposed that the two isolates be classified as representatives of a novel species of , sp. nov. The type strain is MS1-3 (=NBRC 100417=DSM 44801); AS4-2 (=NBRC 100418=DSM 44802) is a reference strain.

Loading

Article metrics loading...

/content/journal/ijsem/10.1099/ijs.0.63189-0
2005-01-01
2021-03-04
Loading full text...

Full text loading...

/deliver/fulltext/ijsem/55/1/ijs550433.html?itemId=/content/journal/ijsem/10.1099/ijs.0.63189-0&mimeType=html&fmt=ahah

References

  1. Felsenstein J. 1985; Confidence limits on phylogenies: an approach using the bootstrap. Evolution 39:783–791 [CrossRef]
    [Google Scholar]
  2. Goodfellow M. 1998; Nocardia and related genera. In Topley and Wilson's Microbiology and Microbial Infections , 9th edn., vol. 2, Systematic Bacteriology pp  463–489 Edited by Balows A., Duerden B. I. London: Arnold;
    [Google Scholar]
  3. Goodfellow M., Lechevalier M. P. 1989; Genus Nocardia Trevisan 1889. In Bergey's Manual of Systematic Bacteriology vol. 4 pp  2350–2361 Edited by Williams S. T., Sharpe M. E., Holt J. P. Baltimore: Williams & Wilkins;
    [Google Scholar]
  4. Goodfellow M., Isik K., Yates E. 1999; Actinomycete systematics: an unfinished synthesis. Nova Acta Leopold NF80:31247–82
    [Google Scholar]
  5. Gordon R. E., Barnett D. A., Handerhan J. E., Pang C. H.-N. 1974; Nocardia coeliaca , Nocardia autotrophica , and the nocardin strain. Int J Syst Bacteriol 24:54–63 [CrossRef]
    [Google Scholar]
  6. Hasegawa T., Takizawa M., Tanida S. 1983; A rapid analysis for chemical grouping of aerobic actinomycetes. J Gen Appl Microbiol 29:319–322 [CrossRef]
    [Google Scholar]
  7. Hayakawa M., Takeuchi T., Toyohiko Y. 1996; Combined use of trimethoprim with nalidixic acid for the selective isolation and enumeration of actinomycetes from soil. Actinomycetologica 10:80–90 [CrossRef]
    [Google Scholar]
  8. Isik K., Chun J., Hah Y. C., Goodfellow M. 1999; Nocardia salmonicida nom. rev., a fish pathogen. Int J Syst Bacteriol 49:833–837 [CrossRef]
    [Google Scholar]
  9. Kageyama A., Yazawa K., Nishimura K., Mikami Y. 2004; Nocardia inohanensis sp. nov., Nocardia yamanashiensis sp. nov. and Nocardia niigatensis sp. nov., isolated from clinical specimens. Int J Syst Evol Microbiol 54:563–569 [CrossRef]
    [Google Scholar]
  10. Kämpfer P., Buczolits S., Jäckel U., Grün-Wollny I., Busse H.-J. 2004; Nocardia tenerifensis sp. nov. Int J Syst Evol Microbiol 54:381–383 [CrossRef]
    [Google Scholar]
  11. Kusunoki S., Ezaki T., Tamesada S., Hatanaka Y., Asano K., Hashimoto Y., Yabuuchi E. 1991; Application of colorimetric microdilution plate hybridization for rapid genetic identification of 22 Mycobacterium species. J Clin Microbiol 29:1596–1603
    [Google Scholar]
  12. Lechevalier M. P., Lechevalier H. 1970; Chemical composition as a criterion in the classification of aerobic actinomycetes. Int J Syst Bacteriol 20:435–443 [CrossRef]
    [Google Scholar]
  13. Lechevalier M. P., De Bièvre C., Lechevalier H. A. 1977; Chemotaxonomy of aerobic actinomycetes: phospholipid composition. Biochem Syst Ecol 5:249–260 [CrossRef]
    [Google Scholar]
  14. Minnikin D. E., O'Donnell A. G., Goodfellow M., Alderson G., Athalye M., Schaal K., Parlett J. H. 1984; An integrated procedure for the extraction of bacterial isoprenoid quinones and polar lipids. J Microbiol Methods 2:233–241 [CrossRef]
    [Google Scholar]
  15. Saintpierre-Bonaccio D., Maldonado L. A., Amir H., Pineau R., Goodfellow M. 2004; Nocardia neocaledoniensis sp. nov., a novel actinomycete isolated from New-Caledonian brown hypermagnesian ultramafic soil. Int J Syst Evol Microbiol 54:599–603 [CrossRef]
    [Google Scholar]
  16. Saitou N., Nei M. 1987; The neighbor-joining method: a new method for reconstructing phylogenetic trees. Mol Biol Evol 4:406–425
    [Google Scholar]
  17. Schaal K. P. 1985; Identification of clinically significant actinomycetes and related bacteria using chemical techniques. In Chemical Methods Bacterial Systematics pp  359–381 Edited by Goodfellow M., Minnikin D. E. London: Academic Press;
    [Google Scholar]
  18. Stackebrandt E., Rainey F. A., Ward-Rainey N. L. 1997; Proposal for a new hierarchic classification system, Actinobacteria classis nov. Int J Syst Bacteriol 47:479–491 [CrossRef]
    [Google Scholar]
  19. Tamura T., Nakagaito Y., Nishii T., Hasegawa T., Stackebrandt E., Yokota A. 1994; A new genus of the order Actinomycetales , Couchioplanes gen. nov., with descriptions of Couchioplanes caeruleus (Horan and Brodsky 1986) comb.nov. and Couchioplanescaeruleus subsp. azureus subsp. nov. Int J Syst Bacteriol 44:193–203 [CrossRef]
    [Google Scholar]
  20. Thompson J. D., Higgins D. G., Gibson T. J. 1994; clustal w: improving the sensitivity of progressive multiple sequence alignment through sequence weighting, position-specific gap penalties and weight matrix choice. Nucleic Acids Res 22:4673–4680 [CrossRef]
    [Google Scholar]
  21. Torres R. D., Oletta C. A., Zlotnik H. 1996; A rapid and gentle method for isolation of genomic DNA from pathogenic Nocardia spp. Clin Diagn Lab Immunol 3:601–604
    [Google Scholar]
  22. Wang L., Zhang Y., Lu Z., Shi Y., Liu Z., Maldonado L., Goodfellow M. 2001; Nocardia beijingensis sp. nov., a novel isolate from soil. Int J Syst Evol Microbiol 51:1783–1788 [CrossRef]
    [Google Scholar]
  23. Wayne L. G., Brenner D. J., Colwell R. R. 9 other authors 1987; International Committee on Systematic Bacteriology. Report of the ad hoc committee on reconciliation of approaches to bacterial systematics. Int J Syst Bacteriol 37:463–464 [CrossRef]
    [Google Scholar]
  24. Yamamura H., Hayakawa M., Iimura Y. 2003a; Application of sucrose-gradient centrifugation for selective isolation of Nocardia spp. from soil. J Appl Microbiol 95:677–685 [CrossRef]
    [Google Scholar]
  25. Yamamura H., Hayakawa M., Nakagawa Y., Iimura Y. 2003b; Species diversity of nocardiae isolated from lake and moat sediment samples. Actinomycetologica 17:44–46 [CrossRef]
    [Google Scholar]
  26. Yamamura H., Hayakawa M., Nakagawa Y., Iimura Y. 2004; Characterization of Nocardia asteroides isolates from different ecological habitats on the basis of repetitive extragenic palindromic-PCR fingerprinting. Appl Environ Microbiol 70:3149–3151 [CrossRef]
    [Google Scholar]
  27. Yassin A. F., Sträubler B., Schumann P., Schaal K. P. 2003; Nocardia puris sp. nov. Int J Syst Evol Microbiol 53:1595–1599 [CrossRef]
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/ijsem/10.1099/ijs.0.63189-0
Loading
/content/journal/ijsem/10.1099/ijs.0.63189-0
Loading

Data & Media loading...

Supplements

Supplementary material 1

PDF

Supplementary material 2

PDF

Most cited this month Most Cited RSS feed

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error