1887

Abstract

During a search for xylan-degrading micro-organisms, a sporulating bacterium was recovered from xylan-containing agar plates exposed to air in a research laboratory (Salamanca University, Spain). The airborne isolate (designated strain XIL14) was identified by 16S rRNA gene sequencing as representing a species most closely related to JCM 9907 (99·3 % sequence similarity) and DSM 3036 (98 % sequence similarity). Phenotypic, chemotaxonomic and DNA–DNA hybridization data indicated that the isolate belongs to a novel species of the genus . Cells of strain XIL14 were motile, sporulating, rod-shaped, Gram-positive and facultatively anaerobic. The predominant cellular fatty acids were anteiso-C and C. The DNA G+C content of strain XIL14 was 50·5 mol%. Growth was observed with many carbohydrates, including xylan, as the only carbon source and gas production was not observed from glucose. Catalase was positive and oxidase was negative. The airborne isolate produced a variety of hydrolytic enzymes, including xylanases, amylases, gelatinase and -galactosidase. DNA–DNA hybridization levels between strain XIL14 and DSM 11733 and DSM 3036 were 43·3 and 36·3 %, respectively. According to the data obtained, strain XIL14 is considered to represent a novel species for which the name sp. nov. is proposed (=LMG 21957=CECT 5839).

Loading

Article metrics loading...

/content/journal/ijsem/10.1099/ijs.0.63173-0
2005-01-01
2024-03-29
Loading full text...

Full text loading...

/deliver/fulltext/ijsem/55/1/ijs550405.html?itemId=/content/journal/ijsem/10.1099/ijs.0.63173-0&mimeType=html&fmt=ahah

References

  1. Ash C., Priest F. G., Collins M. D. 1993; Molecular identification of rRNA group 3 bacilli (Ash, Farrow, Wallbanks and Collins) using a PCR probe test. Antonie van Leeuwenhoek 64:253–260
    [Google Scholar]
  2. Ash C., Priest F. G., Collins M. D. 1994; Paenibacillus gen. nov. In Validation of the Publication of New Names and New Combinations Previously Effectively Published Outside the IJSB. List no. 51. Int J Syst Bacteriol 44:852–853 [CrossRef]
    [Google Scholar]
  3. Ay J., Goetz F., Borriss R., Heinemann U. 1998; Structure and function of the Bacillus hybrid enzyme GluXyn-1: native-like jellyroll fold preserved after insertion of autonomous globular domain. Proc Natl Acad Sci U S A 95:6613–6618 [CrossRef]
    [Google Scholar]
  4. Cashion P., Holder-Franklin M. A., McCully J., Franklin M. 1977; A rapid method for the base ratio determination of bacterial DNA. Anal Biochem 81:461–466 [CrossRef]
    [Google Scholar]
  5. Chun J., Goodfellow M. 1995; A phylogenetic analysis of the genus Nocardia with 16S rRNA sequences. Int J Syst Bacteriol 45:240–245 [CrossRef]
    [Google Scholar]
  6. Claus D., Berkeley R. C. W. 1986; Genus Bacillus Cohn 1872, 174AL . In Bergey's Manual of Systematic Bacteriology pp  1105–1138 Edited by Sneath P. H. A., Mair N. S., Sharpe M. E., Holt J. G. Baltimore: Williams & Wilkins;
    [Google Scholar]
  7. De Ley J., Cattoir H., Reynaerts A. 1970; The quantitative measurement of DNA hybridization from renaturation rates. Eur J Biochem 12:133–142 [CrossRef]
    [Google Scholar]
  8. Doetsch R. N. 1981; Determinative methods of light microscopy. In Manual of Methods for General Bacteriology pp  21–33 Edited by Gerhardt P., Murray R. G. E., Costilow R. N., Nester E. W., Wood W. A., Krieg N. R., Phillips G. B. Washington, DC: American Society for Microbiology;
    [Google Scholar]
  9. Escara J. F., Hutton J. R. 1980; Thermal stability and renaturation of DNA in dimethyl sulfoxide solutions: acceleration of the renaturation rate. Biopolymers 19:1315–1327 [CrossRef]
    [Google Scholar]
  10. Hespell R. B. 1996; Fermentation of xylan, corn fiber, or sugars to acetoin and butanediol by Bacillus polymyxa strains. Curr Microbiol 32:291–296 [CrossRef]
    [Google Scholar]
  11. Huß V. A. R., Festl H., Schleifer K. H. 1983; Studies on the spectrometric determination of DNA hybridization from renaturation rates. Syst Appl Microbiol 4:184–192 [CrossRef]
    [Google Scholar]
  12. Jahnke K. D. 1992; Basic computer program for evaluation of spectroscopic renaturation data from GILFORD System 2600 spectrometer on a PC/XT/AT type personal computer. J Microbiol Methods 15:61–73 [CrossRef]
    [Google Scholar]
  13. Kimura M. 1980; A simple method for estimating evolutionary rates of base substitutions through comparative studies of nucleotide sequences. J Mol Evol 16:111–120 [CrossRef]
    [Google Scholar]
  14. Kumar S., Tamura K., Jakobsen I. B., Nei M. 2001 Molecular Evolutionary Genetics Analysis software Tempe, AZ: Arizona State University;
    [Google Scholar]
  15. Lee H. J., Shin D. J., Cho N. C., Kim H. O., Shin S. Y., Im S. Y., Lee H. B., Chum S. B., Bai S. 2000; Cloning, expression and nucleotide sequences of two xylanase genes from Paenibacillus sp. Biotechnol Lett 22:387–392 [CrossRef]
    [Google Scholar]
  16. Logan N. A., Berkeley R. C. W. 1984; Identification of Bacillus strains using the API system. J Gen Microbiol 130:1871–1882
    [Google Scholar]
  17. Mandel M., Marmur J. 1968; Use of ultraviolet absorbance temperature profile for determining the guanine plus cytosine content of DNA. Methods Enzymol 12B:195–206
    [Google Scholar]
  18. Meehan C., Bjourson A. J., McMullan G. 2001; Paenibacillus azoreducens sp. nov., a synthetic azo dye decolorizing bacterium from industrial wastewater. Int J Syst Evol Microbiol 51:1681–1685 [CrossRef]
    [Google Scholar]
  19. Morales P., Madarro A., Flors A., Sendra J. M., Pérez-González J. A. 1995; Purification and characterization of a xylanase and an arabinofuranosidase from Bacillus polymyxa . Enzyme Microb Technol 17:424–429 [CrossRef]
    [Google Scholar]
  20. Nielsen P., Sorensen J. 1997; Multi-target and medium-independent fungal antagonisms by hydrolytic enzymes in Paenibacillus polymyxa and Bacillus pumilus strains from barley rhizosphere. FEMS Microbiol Ecol 22:183–192 [CrossRef]
    [Google Scholar]
  21. Pearson W. R., Lipman D. J. 1988; Improved tools for biological sequence comparison. Proc Natl Acad Sci U S A 85:2444–2448 [CrossRef]
    [Google Scholar]
  22. Rivas R., Sánchez M., Trujillo M. E., Zurdo-Piñeiro J. L., Mateos P. F., Martínez-Molina E., Velázquez E. 2003a; Xylanimonas cellulosilytica gen. nov., sp. nov. a xylanolytic bacterium isolated from a decayed tree ( Ulmus nigra . Int J Syst Evol Microbiol 53:99–103 [CrossRef]
    [Google Scholar]
  23. Rivas R., Willems A., Subba-Rao N. S., Mateos P. F., Kroppenstedt R., Martínez-Molina E., Gillis M., Velázquez E. 2003b; Description of Devosia neptuniae sp. nov. that nodulates and fixes nitrogen in symbiosis with Neptunia natans , an aquatic legume from India. Syst Appl Microbiol 26:47–54 [CrossRef]
    [Google Scholar]
  24. Saitou N., Nei M. 1987; The neighbor-joining method: a new method for reconstructing phylogenetic trees. Mol Biol Evol 4:406–425
    [Google Scholar]
  25. Shida O., Takagi H., Kadowaki K., Nakamura L.K., Komagata K. 1997a; Transfer of Bacillus alginolyticus , Bacillus chondroitinus , Bacillus curdlanolyticus , Bacillus glucanolyticus , Bacillus kobensis , and Bacillus thiaminolyticus to the genus Paenibacillus and emended description of the genus. Int J Syst Bacteriol 47:289–298 [CrossRef]
    [Google Scholar]
  26. Shida O., Takagi H., Kadowaki K., Nakamura L. K., Komagata K. 1997b; Emended description of Paenibacillus amylolyticus and description of Paenibacillus illinoisensis sp. nov. and Paenibacillus chibensis . Int J Syst Bacteriol 47:299–306 [CrossRef]
    [Google Scholar]
  27. Thompson J. D., Gibson T. J., Plewniak F., Jeanmougin F., Higgins D. G. 1997; The clustal_x windows interface: flexible strategies for multiple sequence alignment aided by quality analysis tools. Nucleic Acid Res 24:4876–4882
    [Google Scholar]
  28. Velázquez E., de Miguel T., Poza M., Rivas R., Rosselló-Mora R., Villa T. G. 2004; Paenibacillus favisporus sp. nov., a xylanolytic bacterium isolated from cow faeces. Int J Syst Evol Microbiol 54:59–64 [CrossRef]
    [Google Scholar]
  29. Wayne L. G., Brenner D. J., Colwell R. R. 9 other authors 1987; Report of the ad hoc committee on reconciliation of approaches to bacterial systematics. Int J Syst Bacteriol 37:463–464 [CrossRef]
    [Google Scholar]
  30. Zamost B. L., Nielsen H. K., Starnes R. L. 1991; Thermostable enzymes for industrial applications. J Ind Microbiol 8:71–82 [CrossRef]
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/ijsem/10.1099/ijs.0.63173-0
Loading
/content/journal/ijsem/10.1099/ijs.0.63173-0
Loading

Data & Media loading...

Supplements

Supplementary material 1

PDF
This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error