A 4-phenyl-3-butenoic acid-producing actinomycete, designated strain VK-A60T, was isolated from a soil sample collected from Koyang, Korea. Morphological and chemical characteristics of the strain were consistent with those of the genus Streptomyces. The cell wall of the strain contains ll-diaminopimelic acid. The predominant fatty acids are anteiso-C15 : 0, iso-C16 : 0 and C16 : 0. The strain formed a distinct monophyletic line within the 16S rRNA gene sequence phylogenetic tree. Analyses of its morphological, physiological and biochemical characteristics, together with random amplified polymorphic DNA and DNA–DNA relatedness data, confirmed that strain VK-A60T represents a novel Streptomyces taxon that is distinguishable from closely related reference strains. Strain VK-A60T (=KCCM 10555T=NBRC 100598T) is proposed as the type strain of a novel species, for which the name Streptomyces koyangensis sp. nov. is proposed.
AltschulS. F.,
MaddenT. L.,
SchäfferA. A.,
ZhangJ.,
ZhangZ.,
MillerW.,
LipmanD. J.1997; Gapped blast and psi-blast: a new generation of protein database search programs. Nucleic Acids Res 25:3389–3402[CrossRef]
BerdyJ.1995; Are actinomycetes exhausted as a source of secondary metabolites?. In Proceedings of the 9th International Symposium on the Biology of Actinomycetes , part I pp 3–23 New York: Allerton;
ChungY. R.,
SungK. C.,
MoH. K.,
SonD. Y.,
NamJ. S.,
ChunJ. S.,
BaeK. S.1999; Kitasatospora cheerisanensis sp. nov., a new species of the genus Kitasatospora that produces an antifungal agent. Int J Syst Bacteriol 49:753–758[CrossRef]
GuckertJ. B.,
RingelbergD. B.,
WhiteD. C.,
HansonR. S.,
BratinaB. J.1991; Membrane fatty acids as phenotypic markers in the polyphasic taxonomy of methylotrophs within the proteobacteria. J Gen Microbiol 137:2631–2641[CrossRef]
HainT.,
Ward-RaineyN.,
KroppenstedtR. M.,
StackebrandtE.,
RaineyF. A.1997; Discrimination of Streptomyces albidoflavus strains based on the size and the number of 16S-23S ribosomal DNA intergenic spacers. Int J Syst Bacteriol 47:202–206[CrossRef]
LabedaD. P.1996; DNA relatedness among verticil-forming Streptomyces species (formerly Streptoverticillium species). Int J Syst Bacteriol 46:699–703[CrossRef]
LabedaD. P.1998; DNA relatedness among the Streptomyces fulvissimus and Streptomyces griseoviridis phenotypic cluster groups. Int J Syst Bacteriol 48:829–832[CrossRef]
LeeJ. Y.2002; Production, purification, and control efficacy against plant diseases of the antibiotics Vka1 and Agr1 from Streptomyces sp. strain VK-A60T and rhizome of Acorus gramineus
. MSc thesis Korea University; Korea:
LeeJ. Y.,
HwangB. K.2002; Diversity of antifungal actinomycetes in various vegetative soils of Korea. Can J Microbiol 48:407–417[CrossRef]
LocciR.1989; Streptomycetes and related genera. In Bergey's Manual of Systematic Bacteriology vol 4 pp 2451–2452 Edited by
WilliamsS. T.,
SharpeM. E.,
HoltJ. G.
Baltimore: Williams & Wilkins;
ManfioG. P.,
Zakrzewska-CzerwinskaJ.,
AtalanE.,
GoodfellowM.1995; Towards minimal standards for the description of Streptomyces species. Biotechnologia 7:8242–253
MarmurJ.,
DotyP.1962; Determination of the base composition of deoxyribonucleic acid from its thermal denaturation temperature. J Mol Biol 5:109–118[CrossRef]
MehlingA.,
WehmeierU. F.,
PiepersbergW.1995; Application of random amplified polymorphic DNA (RAPD) assays in identifying conserved regions of actinomycete genomes. FEMS Microbiol Lett 128:119–126[CrossRef]
OkamiY.,
HottaK.1988; Search and discovery of new antibiotics. In Actinomycetes in Biotechnology pp 33–67 Edited by
GoodfellowM.,
WilliamsS. T.,
MordarskiM.
London: Academic Press;
PridhamT. G.,
HesseltineC. W.,
BenedictR. G.1958; A guide for the classification of streptomycetes according to selected groups. Placement of strains in morphological sections. Appl Microbiol 6:52–79
RobertsM. A.,
CrawfordD. L.2000; Use of randomly amplified polymorphic DNA as a means of developing genus- and strain-specific Streptomyces DNA probes. Appl Environ Microbiol 66:2555–2564[CrossRef]
ShirlingE. B.,
GottliebD.1969; Cooperative description of type cultures of Streptomyces . IV. Species descriptions from the second, third and fourth studies. Int J Syst Bacteriol 19:391–512[CrossRef]
ThompsonJ. D.,
HigginsD. G.,
GibsonT. J.1994; clustal w: improving the sensitivity of progressive multiple sequence alignment through sequence weighting, position-specific gap penalties and weight matrix choice. Nucleic Acids Res 22:4673–4680[CrossRef]
WilliamsS. T.,
GoodfellowM.,
AldersonG.,
WellingtonE. M. H.,
SneathP. H. A.,
SackinM. J.1983; Numerical classification of Streptomyces and related genera. J Gen Microbiol 129:1743–1813