1887

Abstract

A 4-phenyl-3-butenoic acid-producing actinomycete, designated strain VK-A60, was isolated from a soil sample collected from Koyang, Korea. Morphological and chemical characteristics of the strain were consistent with those of the genus . The cell wall of the strain contains -diaminopimelic acid. The predominant fatty acids are anteiso-C, iso-C and C. The strain formed a distinct monophyletic line within the 16S rRNA gene sequence phylogenetic tree. Analyses of its morphological, physiological and biochemical characteristics, together with random amplified polymorphic DNA and DNA–DNA relatedness data, confirmed that strain VK-A60 represents a novel taxon that is distinguishable from closely related reference strains. Strain VK-A60 (=KCCM 10555=NBRC 100598) is proposed as the type strain of a novel species, for which the name sp. nov. is proposed.

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2005-01-01
2019-11-20
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Scanning electron micrograph of spore chains of strain VK-A60 cultured on inorganic salts/starch agar for 13 days. Bar, 2 µm.

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DNA amplification fingerprints of strain VK-A60 and its phylogenetic neighbours analysed using primers AM50, AM62, AM63 and 70-34. Lanes M, 1 kb ladder. Lanes 1?9 show RAPD products of species. Lanes: 1, subsp. IFO 12875 ; 2, DSM 40001 ; 3, DSM 40233 ; 4, DSM 40130 ; 5, DSM 40131 ; 6, DSM 40347 ; 7, DSM 40267; 8, ATCC 25495 ; 9, strain VK-A60 . [PDF](156 KB)

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