1887

INTERNATIONAL JOURNAL OF SYSTEMATIC AND EVOLUTIONARY MICROBIOLOGY logo

Volume 55, Issue 3

gen. nov., sp. nov., a novel marine bacterium of the phylum ‘’ Free

Abstract

An aerobic, Gram-negative, non-fermentative, rod-shaped, motile, orange-pigmented bacterium, UST20020801, was isolated from sea-water samples collected from Port Shelter, Hong Kong, S.A.R., China, in August 2002. The full 16S rRNA gene sequence of this strain shared only 87·5 % similarity with its nearest relative, , a species of the family . However, strain UST20020801 possessed menaquinone-6, a major respiratory quinone of members of the family . This strain contains unique fatty acids such as i15 : 1G, i17 : 19, 2-OH 15 : 0, 15 : 16 and three unknown fatty acids of equivalent chain-length of 11·543, 13·565 and 16·582. Further analysis of its ecophysiology and biochemistry suggests that this strain represents a new genus in the phylum ‘’. The name gen. nov., sp. nov. is proposed. The type strain is UST20020801 (=NRRL B-23963=JCM 12287).

  • Published Online:
SGM
Loading

Article metrics loading...

/content/journal/ijsem/10.1099/ijs.0.63155-0
2005-05-01
2024-04-25
Loading full text...

Full text loading...

/deliver/fulltext/ijsem/55/3/ijs551051.html?itemId=/content/journal/ijsem/10.1099/ijs.0.63155-0&mimeType=html&fmt=ahah

References

  1. Altschul S. F., Madden T. L., Schaffer A. A., Zhang J., Zhang Z., Miller W., Lipman D. J. 1997; Gapped blast and psi-blast: a new generation of protein database search programs. Nucleic Acids Res 25:3389–3402 [CrossRef]
     [Google Scholar]
  2. Barbeyron T., L'Haridon S., Corre E., Kloareg B., Potin P. 2001; Zobellia galactanovorans gen. nov., sp. nov., a marine species of Flavobacteriaceae isolated from a red alga, and classification of [ Cytophaga ] uliginosa (ZoBell and Upham 1944) Reichenbach 1989 as Zobellia uliginosa gen. nov., comb. nov. Int J Syst Evol Microbiol 51985–997 [CrossRef]
  3. Baumann P., Baumann L. 1981; The marine Gram-negative eubacteria: genera Photobacterium Beneckea , Alteromonas , Pseudomonas and Alcaligenes . In The Prokaryotes , vol. 1 pp  1302–1331 Edited by Starr M. P., Stolp H., Trüper H. G., Balows A., Schlegel H. Berlin: Springer;
     [Google Scholar]
  4. Bernardet J.-F., Nakagawa Y., Holmes B. 2002; Proposed minimal standards for describing new taxa of the family Flavobacteriaceae and emended description of the family. Int J Syst Evol Microbiol 52:1049–1070 [CrossRef]
     [Google Scholar]
  5. Bowman J. P., Mancuso Nichols C., Gibson J. A. E. 2003; Algoriphagus ratkowskyi gen. nov., sp. nov., Brumimicrobium glaciale gen. nov., sp. nov., Cryomorpha ignava gen. nov., sp. nov. and Crocinitomix catalasitica gen. nov., sp. nov., novel flavobacteria isolated from various polar habitats. Int J Syst Evol Microbiol 53:1343–1355 [CrossRef]
     [Google Scholar]
  6. Brinkmeyer R., Knittel K., Jürgens J., Weyland H., Amann R., Helmke E. 2003; Diversity and structure of bacterial communities in Arctic versus Antarctic pack ice. Appl Environ Microbiol 69:6610–6619 [CrossRef]
     [Google Scholar]
  7. Bruns A., Rohde M., Berthe-Corti L. 2001; Muricauda ruestringensis gen. nov., sp. nov., a facultatively anaerobic, appendaged bacterium from German North Sea intertidal sediment. Int J Syst Evol Microbiol 51:1997–2006 [CrossRef]
     [Google Scholar]
  8. Cho J. C., Giovannoni S. J. 2003; Croceibacter atlanticus gen. nov., sp. nov., a novel marine bacterium in the family Flavobacteriaceae . Syst Appl Microbiol 26:76–83 [CrossRef]
     [Google Scholar]
  9. Collins M. D. 1994; Isoprenoid quinones. In Chemical Methods in Prokaryotic Systematics pp  265–310 Edited by Goodfellow M., O'Donnell A. G. Chichester: Wiley;
     [Google Scholar]
  10. Collins C. H., Lyne P. M., Grange J. M. 1989 Collins and Lyne's Microbiological Methods London/Boston: Butterworths;
     [Google Scholar]
  11. Cottrell M. T., Kirchman D. L. 2000; Natural assemblages of marine proteobacteria and members of the Cytophaga – Flavobacter cluster consuming low- and high-molecular weight dissolved organic matter. Appl Environ Microbiol 66:1692–1697 [CrossRef]
     [Google Scholar]
  12. Fautz E., Reichenbach H. 1980; A simple test for flexirubin-type pigments. FEMS Microbiol Lett 8:87–91 [CrossRef]
     [Google Scholar]
  13. Felsenstein J. 1985; Confidence limits on phylogenies: an approach using the bootstrap. Evolution 39:783–791 [CrossRef]
     [Google Scholar]
  14. Gosink J. J., Woese C. R., Staley J. T. 1998; Polaribacter gen. nov., with three new species, P.irgensii sp. nov., P. franzmannii sp.nov. and P. filamentus sp. nov.,gas vacuolate polar marine bacteria of the Cytophaga – Flavobacterium – Bacteroides group and reclassification of ‘ Flectobacillus glomeratus ’ as Polaribacter glomeratus comb. nov. Int J Syst Bacteriol 48:223–235 [CrossRef]
     [Google Scholar]
  15. Ivanova E. P., Nedashkovskaya O. I., Chun J. 7 other authors 2001; Arenibacter gen. nov., new genus of the family Flavobacteriaceae and description of a new species, Arenibacter latericius sp. nov. Int J Syst Evol Microbiol 51:1987–1995 [CrossRef]
     [Google Scholar]
  16. Johansen J. E., Nielsen P., Sjøholm C. 1999; Description of Cellulophaga baltica gen.nov., sp. nov. and Cellulophaga fucicola gen. nov., sp. nov. and reclassification of [ Cytophaga ] lytica to Cellulophaga lytica gen. nov., comb. nov. Int J Syst Bacteriol 491231–1240 [CrossRef]
  17. Johnson J. L. 1985; Determination of DNA base composition. Methods Microbiol 18:1–31
     [Google Scholar]
  18. Kimura M. 1980; A simple method for estimating evolutionary rates of base substitutions through comparative studies of nucleotide sequences. J Mol Evol 16:111–120 [CrossRef]
     [Google Scholar]
  19. Kumar S., Tamura K., Jakobsen I. B., Nei M. 2001; mega2: molecular evolutionary genetics analysis software. Bioinformatics 17:1244–1245 [CrossRef]
     [Google Scholar]
  20. Lane D. J. 1991; 16S/23S rRNA sequencing. In Nucleic Acid Techniques in Bacterial Systematics pp  115–175 Edited by Stackebrandt E., Goodfellow M. Chichester: John Wiley;
     [Google Scholar]
  21. Lewin R. A., Lounsbery D. M. 1969; Isolation, cultivation and characterization of flexibacteria. J Gen Microbiol 58:145–170 [CrossRef]
     [Google Scholar]
  22. Mesbah M., Premachandran U., Whitman W. B. 1989; Precise measurement of the G+C content of deoxyribonucleic acid by high-performance liquid chromatography. Int J Syst Bacteriol 39:159–167 [CrossRef]
     [Google Scholar]
  23. Nakagawa Y., Yamasato K. 1993; Phylogenetic diversity of the genus Cytophaga revealed by 16S rRNA sequencing and menaquinone analysis. J Gen Microbiol 139:1155–1161 [CrossRef]
     [Google Scholar]
  24. Nedashkovskaya O. I., Suzuki M., Vysotskii M. V., Mikhailov V. V. 2003; Vitellibacter vladivostokensis gen. nov., sp. nov., a new member of the phylum Cytophaga – Flavobacterium – Bacteroides . Int J Syst Evol Microbiol 53:1281–1286 [CrossRef]
     [Google Scholar]
  25. Norris J. R., Ribbons D. W., Varma A. K. (editors) 1985 Methods in Microbiology , vol. 18 London: Academic Press;
     [Google Scholar]
  26. Reichenbach H. 1989; Family 1. Cytophagaceae Stanier 1940, 630AL emend. In Bergey's Manual of Systematic Bacteriology vol 3 pp  2015–2082 Edited by Staley J. T., Bryant M. P., Pfennig N., Holt J. G. Baltimore: Williams & Wilkins;
     [Google Scholar]
  27. Robinson D. G., Ehlers U., Herken R., Herrmann B., Mayer F., Schürmann F.-W. 1987; Methods for SEM. In Methods of Preparation for Electron Microscopy: an Introduction for the Biomedical Sciences pp  145–165 Berlin: Springer;
     [Google Scholar]
  28. Saitou N., Nei M. 1987; The neighbor-joining method: a new method for reconstructing phylogenetic trees. Mol Biol Evol 4:406–425
     [Google Scholar]
  29. Smibert R. M., Krieg N. R. 1994; Phenotypic characterization. In Methods for General and Molecular Bacteriology pp  607–654 Edited by Gerhardt P., Murray R. G. E., Wood W. A., Krieg N. R. Washington, DC: American Society for Microbiology;
     [Google Scholar]
  30. Steyn P. L., Segers P., Vancanneyt M., Sandra P., Kersters K., Joubert J. J. 1998; Classification of heparinolytic bacteria into a new genus, Pedobacter , comprising four species: Pedobacter heparinus comb.nov., Pedobacter piscium comb. nov., Pedobacter africanus sp. nov. and Pedobacter saltans sp. nov.Proposal of the family Sphingobacteriaceae . Int J Syst Bacteriol 48:165–177 [CrossRef]
     [Google Scholar]
  31. Suzuki M., Nakagawa Y., Harayama S., Yamamoto S. 2001; Phylogenetic analysis and taxonomic study of marine Cytophaga -like bacteria: proposal for Tenacibaculum gen. nov. with Tenacibaculum maritimum comb.nov. and Tenacibaculum ovolyticum comb. nov., and description of Tenacibaculum mesophilum sp.nov. and Tenacibaculum amylolyticum sp. nov. Int J Syst Evol Microbiol 51:1639–1652 [CrossRef]
     [Google Scholar]
  32. Thompson J. D., Gibson T. J., Plewniak F., Jeanmougin F., Higgins D. G. 1997; The clustal_x windows interface: flexible strategies for multiple sequence alignment aided by quality analysis tools. Nucleic Acids Res 25:4876–4882 [CrossRef]
     [Google Scholar]
  33. Van de Peer Y., De Wachter R. 1994; treecon for Windows: a software package for the construction and drawing of evolutionary trees for the Microsoft Windows environment. Comput Appl Biosci 10:569–570
     [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/ijsem/10.1099/ijs.0.63155-0
Loading
Owenweeksia hongkongensis gen. nov., sp. nov., a novel marine bacterium of the phylum ‘Bacteroidetes’
Int J Syst Evol Microbiol 55, 1051 (2005); https://doi.org/10.1099/ijs.0.63155-0
/content/journal/ijsem/10.1099/ijs.0.63155-0
/content/journal/ijsem/10.1099/ijs.0.63155-0
Loading

Data & Media loading...

Supplements

Supplementary material 1

PDF

Most cited Most Cited RSS feed

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error