1887

Abstract

A novel anaerobic, thermophilic and cellulolytic bacterium (strain CSK1) was isolated from a cellulose-degrading bacterial community. On the basis of 16S rRNA gene sequence similarity, strain CSK1 was mapped to cluster III of the genus . Strain CSK1 is closely related to (96·2 %) and (95·1 %). Strain CSK1 is a non-motile, spore-forming, straight or slightly curved rod. The optimum temperature and initial pH for its growth and cellulose degradation are 50–55 °C and pH 7·5. Strain CSK1 grew under a gas phase containing up to 4 % O. Phylogenetic and phenotypic analyses support the differentiation of strain CSK1 from its closest relatives. Strain CSK1 therefore represents a novel species, for which the name sp. nov. is proposed, with CSK1 (=DSM 16021=IAM 15070) as the type strain.

Loading

Article metrics loading...

/content/journal/ijsem/10.1099/ijs.0.63148-0
2004-11-01
2019-10-19
Loading full text...

Full text loading...

/deliver/fulltext/ijsem/54/6/ijs542043.html?itemId=/content/journal/ijsem/10.1099/ijs.0.63148-0&mimeType=html&fmt=ahah

References

  1. Altschul, S. F., Madden, T. L., Schaffer, A. A., Zhang, J., Zhang, Z., Miller, W. & Lipman, D. J. ( 1997; ). Gapped blast and psi-blast: a new generation of protein database search programs. Nucleic Acids Res 25, 3389–3402.[CrossRef]
    [Google Scholar]
  2. Collins, M. D., Lawson, P. A., Willems, A., Cordoba, J. J., Fernandez-Garayzabal, J., Garcia, P., Cai, J., Hippe, H. & Farrow, J. A. E. ( 1994; ). The phylogeny of the genus Clostridium: proposal of five new genera and eleven new species combinations. Int J Syst Bacteriol 44, 812–826.[CrossRef]
    [Google Scholar]
  3. Coughlan, M. P. & Mayer, F. ( 1992; ). The cellulose-decomposing bacteria and their enzyme systems. In The Prokaryotes, 2nd edn, pp. 460–516. Edited by A. Balows, H. G. Trüper, M. Dworkin, W. Harder & K. H. Schleifer. New York: Springer.
  4. Devereux, R. & Willis, S. G. ( 1995; ). Amplification of ribosomal RNA sequences. In Molecular Microbial Ecology Manual, 3.3.1, pp. 1–11. Edited by A. D. L. Akkermans, J. D. van Elsas & F. J. de Bruijn. Dordrecht: Kluwer.
  5. Drent, W. J., Lahpor, G. A., Wiegant, W. M. & Gottschal, J. C. ( 1991; ). Fermentation of inulin by Clostridium thermosuccinogenes sp. nov., a thermophilic anaerobic bacterium isolated from various habitats. Appl Environ Microbiol 57, 455–462.
    [Google Scholar]
  6. Ezaki, T., Hashimoto, Y. & Yabuuchi, E. ( 1989; ). Fluorometric deoxyribonucleic acid-deoxyribonucleic acid hybridization in microdilution wells as an alternative to membrane filter hybridization in which radioisotopes are used to determine genetic relatedness among bacterial strains. Int J Syst Bacteriol 39, 224–229.[CrossRef]
    [Google Scholar]
  7. Felsenstein, J. ( 1985; ). Confidence limits on phylogenies: an approach using the bootstrap. Evolution 39, 783–791.[CrossRef]
    [Google Scholar]
  8. Fukumori, F., Kudo, T., Sashihara, N., Nagata, Y., Ito, K. & Horikoshi, K. ( 1989; ). The third cellulase of alkalophilic Bacillus sp. strain N-4: evolutionary relationships within the cel gene family. Gene 76, 289–298.[CrossRef]
    [Google Scholar]
  9. Haruta, S., Cui, Z., Huang, Z., Li, M., Ishii, M. & Igarashi, Y. ( 2002; ). Construction of a stable microbial community with high cellulose-degradation ability. Appl Microbiol Biotechnol 59, 529–534.[CrossRef]
    [Google Scholar]
  10. Hayashi, H., Takehara, M., Hattori, T., Kimura, T., Karita, S., Sakka, K. & Ohmiya, K. ( 1999; ). Nucleotide sequences of two contiguous and highly homologous xylanase genes xynA and xynB and characterization of XynA from Clostridium thermocellum. Appl Microbiol Biotechnol 51, 348–357.[CrossRef]
    [Google Scholar]
  11. Heijthuijsen, J. H. F. G. & Hansen, T. A. ( 1986; ). Interspecies hydrogen transfer in co-cultures of methanol-utilizing acidogens and sulphate-reducing or methanogenic bacteria. FEMS Microbiol Ecol 7, 387–429.
    [Google Scholar]
  12. Holdeman, L. V., Cato, E. P. & Moore, W. E. C. ( 1977; ). Anaerobe Laboratory Manual, 4th edn. Blacksburg, VA: Anaerobe Laboratory, Virginia Polytechnic Institute and State University.
  13. Karnholz, A., Küsel, K., Gößner, A., Schramm, A. & Drake, H. L. ( 2002; ). Tolerance and metabolic response of acetogenic bacteria toward oxygen. Appl Environ Microbiol 68, 1005–1009.[CrossRef]
    [Google Scholar]
  14. Kumar, S., Tamura, K., Jakobsen, I. B. & Nei, M. ( 2001; ). mega2: molecular evolutionary genetics analysis software. Bioinformatics 17, 1244–1245.[CrossRef]
    [Google Scholar]
  15. Küsel, K. & Drake, H. L. ( 1995; ). Effects of environmental parameters on the formation and turnover of acetate by forest soils. Appl Environ Microbiol 61, 3667–3675.
    [Google Scholar]
  16. Küsel, K., Karnholz, A., Trinkwalter, T., Devereux, R., Acker, G. & Drake, H. L. ( 2001; ). Physiological ecology of Clostridium glycolicum RD-1, an aerotolerant acetogen isolated from sea grass roots. Appl Environ Microbiol 67, 4734–4741.[CrossRef]
    [Google Scholar]
  17. Lewis, S. M., Montgomery, L., Garleb, K. A., Berger, L. L. & Fahey, G. C., Jr ( 1988; ). Effects of alkaline hydrogen peroxide treatment on in vitro degradation of cellulosic substrates by mixed ruminal microorganisms and Bacteroides succinogenes S85. Appl Environ Microbiol 54, 1163–1169.
    [Google Scholar]
  18. Mesbah, M., Premachandran, U. & Whitman, W. B. ( 1989; ). Precise measurement of the G+C content of deoxyribonucleic acid by high-performance liquid chromatography. Int J Syst Bacteriol 39, 159–167.[CrossRef]
    [Google Scholar]
  19. Ng, T. K., Weimer, P. J. & Zeikus, J. G. ( 1977; ). Cellulolytic and physiological properties of Clostridium thermocellum. Arch Microbiol 114, 1–7.[CrossRef]
    [Google Scholar]
  20. Odom, J. M. & Wall, J. D. ( 1983; ). Photoreduction of H2 from cellulose by an anaerobic bacterial coculture. Appl Environ Microbiol 45, 1300–1395.
    [Google Scholar]
  21. Saddler, J. N. & Chan, M. K. H. ( 1982; ). Optimization of Clostridium thermocellum growth on cellulose and pretreated wood substrates. Appl Microbiol Biotechnol 16, 99–104.[CrossRef]
    [Google Scholar]
  22. Saitou, N. & Nei, M. ( 1987; ). The neighbor-joining method: a new method for reconstructing phylogenetic trees. Mol Biol Evol 4, 406–425.
    [Google Scholar]
  23. Taillisz, P., Girard, H., Millet, J. & Beguin, P. ( 1989; ). Enhanced cellulose fermentation by an asporogenous and ethanol-tolerant mutant of Clostridium thermocellum. Appl Environ Microbiol 55, 207–211.
    [Google Scholar]
  24. Tanaka, K., Ding, M. Y., Helaleh, M. I. H. & 7 other authors ( 2002; ). Vacancy ion-exclusion chromatography of carboxylic acids on a weakly acidic cation-exchange resin. J Chromatogr A 956, 209–214.[CrossRef]
    [Google Scholar]
  25. Thompson, J. D., Gibson, T. J., Plewniak, F., Jeanmougin, F. & Higgins, D. G. ( 1997; ). The clustal_x Windows interface: flexible strategies for multiple sequence alignment aided by quality analysis tools. Nucleic Acids Res 25, 4876–4882.[CrossRef]
    [Google Scholar]
  26. Yang, J. C., Chynoweth, D. P., Williams, D. S. & Li, A. ( 1990; ). Clostridium aldrichii sp. nov., a cellulolytic mesophile inhabiting a wood-fermenting anaerobic digester. Int J Syst Bacteriol 40, 268–272.[CrossRef]
    [Google Scholar]
  27. Zhu, H., Qu, F. & Zhu, L. H. ( 1993; ). Isolation of genomic DNAs from plants, fungi and bacteria using benzyl chloride. Nucleic Acids Res 21, 5279–5280.[CrossRef]
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/ijsem/10.1099/ijs.0.63148-0
Loading
/content/journal/ijsem/10.1099/ijs.0.63148-0
Loading

Data & Media loading...

Most Cited This Month

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error